3D high- and super-resolution imaging using single-objective SPIM

Galland, Rémi; Grenci, Gianluca; Aravind, Ajay; Viasnoff, Virgile; Studer, Vincent; Sibarita, Jean‐Baptiste

doi:10.1038/nmeth.3402

Cited by 186 publications

(175 citation statements)

References 28 publications

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“…MPoVM is a relatively simple and cost-effective approach, not depending on expensive and vulnerable moving parts, that can be readily implemented on existing fluorescence microscopes. MPoVM can be extended with additional functionality, for example to optically extend the depth of field to up to tens of micrometers [29][30][31][32], or combined with light-sheet excitation and detection [33]. This latter approach, however, will substantially increase the complexity of the current setup.…”

Section: Discussionmentioning

confidence: 99%

High-resolution real-time dual-view imaging with multiple point of view microscopy

Mangeol¹

2016

Biomed. Opt. Express

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Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions. 2. R. Tomer, K. Khairy, F. Amat, and P. J. Keller, "Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy," Nat. Methods 9(7), 755-763 (2012). 3. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, "Multiview light-sheet microscope for rapid in toto imaging," Nat. Methods 9(7), 730-733 (2012) imaging using single-objective SPIM," Nat. Methods 12(7), 641-644 (2015). 34. S.-I. Chang and J.-B. Yoon, "Shape-controlled, high fill-factor microlens arrays fabricated by a 3D diffuser lithography and plastic replication method," Opt. Express 12(25), 6366-6371 (2004).

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Section: Discussionmentioning

confidence: 99%

High-resolution real-time dual-view imaging with multiple point of view microscopy

Mangeol¹

2016

Biomed. Opt. Express

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“…Finally, higher resolutions (NA > 1.1) can be obtained by using a conventional microscope with a single objective, which creates the LS and collects the fluorescence generated. Examples of these are the OPM technique [23,77,78] and the soSPIM [79][80][81][82] techniques that have been used for observing whole cells and cell aggregates in combination with superresolution (SR) localization microscopy.…”

Section: 1e Lsfm Allows Imaging Of Single Cells At High Resolutionmentioning

confidence: 99%

“…This has allowed combining different excitation regimes (linear and nonlinear) [64] in one single instrument. In addition, LSFM has been combined with SR techniques such as STORM [48,[80][81][82], STED [92], and structured illumination [69,75,93]. The versatility of LSFM also allows combining it with more established techniques, such as FLIM [94][95][96], FRET [96], hyperspectral fluorescence [97], Raman [98][99][100][101], and OPT [102,103].…”

Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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“…These molds are used to replicate the structures on biocompatible materials (such as polydimethylsiloxane, commonly known as PDMS) using soft lithography, e.g., cast molding. These biocompatible devices can be applied as protein stamping tools, 11 microfluidic/microoptical devices, 12,13 and quite often used as substrates for biological studies. One such application is the use of micro-PDMS pillars as force-sensing tools in traction force microscopy.…”

Section: Introductionmentioning

confidence: 99%

Low-power, low-pressure reactive-ion etching process for silicon etching with vertical and smooth walls for mechanobiology application

Ashraf

Sundararajan

Grenci

2017

J. Micro/Nanolith. MEMS MOEMS

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Abstract. We report our findings in developing a low-power etching recipe using a newly acquired reactive-ion etching (RIE) tool (RIE-10NR, Samco, Japan), with the aim of achieving smooth and vertical sidewalls in micropatterned silicon substrate. We used a combination of CF 4 , SF 6 , and O 2 gases, which at low power (30 W) and low pressure (2 Pa) allowed for vertical silicon etching (aspect ratio ∼2). We used photoresist and silicon oxide as the etching masks. As it is a continuous etching process, scalloping effects were not present, which is contrary to the process done with an inductively coupled plasma-based "Bosch" approach. We also show a successful use of these microstructures as master mold in soft-lithographic techniques for producing devices in elastomeric materials that have applications in mechanobiology. To the best of our knowledge, the recipe we present here has the lowest combination of power and pressure for etching silicon with vertical profile using a standard, parallel plates RIE tool. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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3D high- and super-resolution imaging using single-objective SPIM

Cited by 186 publications

References 28 publications

High-resolution real-time dual-view imaging with multiple point of view microscopy

High-resolution real-time dual-view imaging with multiple point of view microscopy

Light-sheet microscopy: a tutorial

Low-power, low-pressure reactive-ion etching process for silicon etching with vertical and smooth walls for mechanobiology application

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