3D direct writing egg white hydrogel promotes diabetic chronic wound healing via self-relied bioactive property

Guo, Lingling; Niu, Xinli; Chen, Xihang; Lu, Feng; Gao, Jianhua; Chang, Qiang

doi:10.1016/j.biomaterials.2022.121406

Cited by 32 publications

(29 citation statements)

References 65 publications

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“…The formation of the EWG0 hydrogel depended on the physical cross-linking with only weak binding between protein chains, giving water molecules easy access to the hydrogel interior and resulting in high expansibility. Moreover, it was found that the EWG0 hydrogel was gradually degraded and ruptured with extended soaking time, which was because the water molecules destroyed the three-dimensional structure with weak intermolecular forces [ 45 ]. It is well known that the degree of cross-linking directly affects the water absorption of a hydrogel [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Preparation and Properties of Egg White Dual Cross-Linked Hydrogel with Potential Application for Bone Tissue Engineering

et al. 2022

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In this study, an egg white dual cross-linked hydrogel was developed based on the principle that the external stimulus can denature proteins and cause them to aggregate, forming hydrogel. The sodium hydroxide was used to induce gelation of the egg white protein, subsequently introducing calcium ions to cross-link with protein chains, thereby producing a dual cross-linked hydrogel. The characteristics of the dual cross-linked hydrogels—including the secondary structure, stability, microstructure, swelling performance, texture properties, and biosafety—were investigated to determine the effects of calcium ion on the egg white hydrogel (EWG) and evaluate the potential application in the field of tissue engineering. Results showed that calcium ions could change the β-sheet content of the protein in EWG after soaking it in different concentrations of CaCl2 solution, leading to changes in the hydrogen bonds and the secondary structure of polypeptide chains. It was confirmed that calcium ions promoted the secondary cross-linking of the protein chain, which facilitated polypeptide folding and aggregation, resulting in enhanced stability of the egg white dual cross-linked hydrogel. Furthermore, the swelling capacity of the EWG decreased with increasing concentration of calcium ions, and the texture properties including hardness, cohesiveness and springiness of the hydrogels were improved. In addition, the calcium cross-linked EWG hydrogels exhibited biocompatibility and cell-surface adhesion in vitro. Hence, this work develops a versatile strategy to fabricate dual cross-linked protein hydrogel with biosafety and cell-surface adhesion, and both the strategy and calcium-egg white cross-linked hydrogels have potential for use in bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Preparation and Properties of Egg White Dual Cross-Linked Hydrogel with Potential Application for Bone Tissue Engineering

et al. 2022

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“…The preparation and characterization of egg white hydrogel were conducted according to protocols in our previous study with minor modifications ( Chang et al, 2019 ; Guo et al, 2022 ). In previous studies, we demonstrated that the sodium hydroxide gel method has similar material and biological properties between different egg species (white-shell eggs, brown-shell eggs and duck eggs) ( Guo et al, 2022 ). Considering the consistency and cost of this experiment, EW was extracted from fresh white-shell eggs and fully shaken evenly with a vortex vibrator to form a uniform EW liquid.…”

Section: Methodsmentioning

confidence: 99%

“…SEWH (EWH soaked in DMEM for secondary cross-linking) and 3D-SEWH utilized intrinsic bioactive components to stimulate the activity of Fbs and ASCs in vitro. Furthermore, 3D-SEWH showed better wound healing effect and angiogenesis than SEWH (Guo et al, 2022). However, the efficacy and clinical translation of EWH alone are still limited compared to relatively mature ASCs therapies.…”

Section: Introductionmentioning

confidence: 99%

Direct three-dimensional printed egg white hydrogel wound dressing promotes wound healing with hitching adipose stem cells

Wang

Niu

et al. 2022

Front. Bioeng. Biotechnol.

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Current wound dressing based on hydrogel offers a promising way to accelerate the healing process, yet great challenges remain in the development of a highly integrated and efficient platform with the combination of therapeutic biomolecules and stem cells. Herein, a natural hydrogel wound dressing from egg white can be conveniently obtained by feasible physical crosslinking, the prepared hydrogel dressing features interconnected microporous channels, direct 3D printing, cytocompatibility, and intrinsic biomolecules to advance cell behavior. The 3D printed egg white hydrogels promote the adhesion and proliferation of adipose-derived stem cells (ASCs) without obvious cytotoxicity. In addition, this integrated hydrogel platform accompanied with adipose-derived stem cells accelerates wound healing through the enhancement of fibroblast proliferation, angiogenesis, and collagen rearrangement in the wound bed. The egg white hydrogel provides an effective wound caring product possessing low cost, easy availability along with ready manufacturing, and advanced therapeutic effect, which may be extended for the management of chronic or other complicated wounds.

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“…Studies involving egg whites have shown promise, and were originated from a treatment widely used in antiquity as a poultice for dressings on burns and wound healing [ 169 ]. Currently, the search for biodegradable, easily obtainable, and low-cost hydrogels has attracted the industry and pharmaceutical research.…”

Section: D-printed Materials At Different Stages Of Wound Healingmentioning

confidence: 99%

3D Printing as a Technological Strategy for the Personalized Treatment of Wound Healing

Uchida

Bruschi

2023

AAPS PharmSciTech

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Wound healing is a dynamic process which involves stages of hemostasis, inflammation, proliferation and remodeling. Any error in this process results in abnormal wound healing, generating financial burdens for health systems and even affecting the physical and mental health of the patient. Traditional dressings do not meet the complexities of ideal treatment in all types of wounds. For this reason, in the last decades, different materials for drug delivery and for the treatment of wounds have been proposed reaching novel level of standards, such as 3D printing techniques. The use of natural or synthetic polymers, and the correct design of these printed products loaded with cells and/or combined with active compounds, can generate an effective system for the treatment of wounds, improving the healing process and generating customized dressings according to the patient needs. This manuscript provides a comprehensive review of different types of 3D printing techniques, as well as its use in wound healing and its different stages, including the advantages and limitations of additive manufacturing and future perspectives. Graphical abstract

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3D direct writing egg white hydrogel promotes diabetic chronic wound healing via self-relied bioactive property

Cited by 32 publications

References 65 publications

Preparation and Properties of Egg White Dual Cross-Linked Hydrogel with Potential Application for Bone Tissue Engineering

Preparation and Properties of Egg White Dual Cross-Linked Hydrogel with Potential Application for Bone Tissue Engineering

Direct three-dimensional printed egg white hydrogel wound dressing promotes wound healing with hitching adipose stem cells

3D Printing as a Technological Strategy for the Personalized Treatment of Wound Healing

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