3D culture of murine neural stem cells on decellularized mouse brain sections

Waele, Jorrit De; Reekmans, Kristien; Daans, Jasmijn; Goossens, Herman; Berneman, Zwi N.; Ponsaerts, Peter

doi:10.1016/j.biomaterials.2014.11.025

Cited by 78 publications

(59 citation statements)

References 46 publications

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“…However, the most common approach to create decellularized sections is to first cut the tissue into the desired thickness and then decellularize the slices of tissue. This approach has been used for tendon, brain, kidney, lung, liver, corneal stroma, and dermis [25–30]. Decellularized materials can also be created with sizes between whole organ decellularization and thin slices, for example 4 × 4 × 3 mm cubes of bone [31] or 4 × 2 × 0.5 cm pieces of musculofascial tissue [32].…”

Section: Preparation Of Decellularized Materialsmentioning

confidence: 99%

“…Waele et al used decellularized mouse brain sections as a platform to culture rat neural stem cells (NSCs). They were able to maintain the NSC phenotype without further differentiation with media supplements such as epidermal growth factor and human basic fibroblast growth factor [25]. This may suggest that cues from nervous system ECM that were shown to promote differentiation in the previously mentioned studies can be overridden simply with media supplements.…”

Section: Ecm Effects On Cell Differentiationmentioning

confidence: 99%

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Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

142

104

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Decellularized tissues have become a common regenerative medicine platform with multiple materials being researched in academic laboratories, tested in animal studies, and used clinically. Ideally, when a tissue is decellularized the native cell niche is maintained with many of the structural and biochemical cues that naturally interact with the cells of that particular tissue. This makes decellularized tissue materials an excellent platform for providing cells with the signals needed to initiate and maintain differentiation into tissue-specific lineages. The extracellular matrix (ECM) that remains after the decellularization process contains the components of a tissue specific microenvironment that is not possible to create synthetically. The ECM of each tissue has a different composition and structure and therefore has unique properties and potential for affecting cell behavior. This review describes the common methods for preparing decellularized tissue materials and the effects that decellularized materials from different tissues have on cell phenotype.

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Section: Preparation Of Decellularized Materialsmentioning

confidence: 99%

Section: Ecm Effects On Cell Differentiationmentioning

confidence: 99%

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

142

104

View full text Add to dashboard Cite

show abstract

“…An extracellular matrix is commonly used for the therapeutic reconstruction and repair of many tissues, including musculotendinous tissues8910, the lower urinary tract11, the kidney12, the myocardium1314, the oesophagus, the peripheral nerves15, and the central nervous system1617, among others. Many recent studies have used the acellular spinal cord (ASC) extracellular matrix as a cell carrier to investigate its therapeutic effect on neural recovery18.…”

mentioning

confidence: 99%

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Tian

et al. 2016

Sci Rep

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Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions. The bFGF-ASC complex was further encapsulated into a heparin modified poloxamer (HP) solution to prepare atemperature-sensitive hydrogel (bFGF-ASC-HP). bFGF release from the ASC-HP hydrogel was more slower than that from the bFGF-ASC complex alone. An in vitro cell survival study showed that the bFGF-ASC-HP hydrogel could more effectively promote the proliferation of PC12 cells than a bFGF solution, with an approximate 50% increase in the cell survival rate within 24 h (P < 0.05). Compared with the bFGF solution, bFGF-ASC-HP hydrogel displayed enhanced inhibition of glial scars and obviously improved the functional recovery of the SCI model rat through regeneration of nerve axons and the differentiation of the neural stem cells. In summary, an ASC-HP hydrogel might be a promising carrier to deliver bFGF to an injured spinal cord.

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“…DECM scaffolds improve cell growth, migration, differentiation and can positively influence tissue repair and remodeling [3][4][5][6][7]. DECM from a variety of tissues such as skeletal muscle [8][9][10], brain [11,12], urinary bladder [2,5,13], small intestinal submucosa [14][15][16], liver [4,[17][18][19], skin/adipose tissue [20][21][22], blood vessels [23][24][25], heart valves [26,27] and tendons [28] have been used for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Decellularized extracellular matrices for tissue engineering applications

Elmashhady¹,

Kraemer²,

Patel³

et al. 2017

Electrospinning

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Decellularization removes cellular antigens while preserving the ultrastructure and composition of extracellular matrix (ECM). Decellularized ECM (DECM) scaffolds have been widely used in various tissue engineering applications with varying levels of success. The mechanical, architectural and bioactive properties of a DECM scaffold depend largely on the method of decellularization and dictate its clinical efficacy. This article highlights the advantages and challenges associated with the clinical use of DECM scaffolds. Poor mechanical strength is a significant disadvantage of some DECM scaffolds in the repair of load-bearing tissues as well as critical-size defects, where long-term mechanical support is required for the regenerating tissue. Combining DECM scaffolds with synthetic biocompatible polymers could provide a useful strategy to circumvent the issues of poor mechanical stability. This article reviews studies that have combined DECM scaffolds from various tissues with synthetic polymers to create hybrid scaffolds using electrospinning. These hybrid scaffolds provide a mechanical backbone while retaining the bioactive properties of DECM, thus offering a significant advantage for tissue engineering and regenerative medicine applications.

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3D culture of murine neural stem cells on decellularized mouse brain sections

Cited by 78 publications

References 46 publications

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Decellularized extracellular matrices for tissue engineering applications

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