3D co-culture of hematopoietic stem and progenitor cells and mesenchymal stem cells in collagen scaffolds as a model of the hematopoietic niche

Leisten, Isabelle; Kramann, Rafael; Ferreira, Mónica S. Ventura; Bovi, Manfred; Neuß, Sabine; Ziegler, Patrick; Wagner, Wolfgang; Knüchel, Ruth; Schneider, Rebekka K.

doi:10.1016/j.biomaterials.2011.11.034

Cited by 160 publications

(129 citation statements)

References 28 publications

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“…Tissue-engineered approaches to the HSC niche are frequently based on hydrogels [32,[37][38][39][40]. However, the use of solid scaffolds with higher mechanical properties allows more complex approaches.…”

Section: Discussionmentioning

confidence: 99%

“…MSCs are sourced from multiple tissues, including bone marrow [49] and placenta [50]. Bone marrow-derived MSCs (bmMSCs) have been used to develop in vitro models of the HSC niche [32,38,51]. However, plMSCs are more accessible than bmMSCs in terms of their isolation procedures and cell numbers per donor [52].…”

Section: Discussionmentioning

confidence: 99%

“…To decipher the ECM production by hOBs and plMSCs grown on scaffolds, the expression and deposition of FN, VN, N-cad and collagen type-I (Col-I), representative of ECM proteins that regulate haematopoiesis at the endosteal site [10,14,15,32], was determined by confocal microscopy and Western blot analysis. FN was abundant in all conditions tested and increased in hOBs compared to plMSCs (Fig.…”

Section: Characterisation Of Ecm Proteins Secreted By Hobs and Plmscsmentioning

confidence: 99%

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Characterisation Of Ecm Proteins Secreted By Hobs and Plmscsmentioning

confidence: 99%

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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“…In this study, hematopoietic stem cells show differentiation towards myeloid series (CD13 + , CAE + and MPO + ) and are also CD45 + . 64 Myeloid differentiation of HSCs in all the conditions in the presence of MSCs was significantly increased compared to stromal cell free conditions. 65 Research shows that cord blood mesenchymal stem cells are not appropriate to maintain the CD34 + /CD38 -phenotype during long term culture.…”

mentioning

confidence: 93%

“…However, cord blood MSCs significantly increase the proliferation of HSCs and promote their differentiation. 64 Mesenchymal stem cells support the cell proliferation in high passages while they maintain the CD34 + phenotype in early passages in high number of cell divisions, indicating increased self-renewal versus differentiation in early passages, and are thus more appropriate for the expansion of CD34 + HSCs in vitro. CD34 + cell differentiation in co-culture with stromal cells occurs only after several cell divisions, and it is noteworthy that this is related to asymmetric cell division.…”

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confidence: 97%