Mimicking the Hematopoietic Stem Cell Niche by Biomaterials

Müller, Eike; Ansorge, Michael; Werner, Carsten; Pompe, Tilo

doi:10.1002/9781118843499.ch16

Cited by 3 publications

(3 citation statements)

References 103 publications

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“…A promising strategy to increase HSC expansion ex vivo is mimicking the HSC microenvironment by the integration of bioengineering approaches with our constantly expanding knowledge of the soluble signals involved in hematopoiesis 13 14 . For instance, growth factors, regulatory cytokines and chemokines essential for the tightly balanced intercellular crosstalk regulating HSC fate have been successfully identified using protein microarray technologies 15 16 17 .…”

mentioning

confidence: 99%

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Müller

Wang

Qiao

et al. 2016

Sci Rep

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Homeostasis of hematopoietic stem cells (HSC) in the mammalian bone marrow stem cell niche is regulated by signals of the local microenvironment. Besides juxtacrine, endocrine and metabolic cues, paracrine and autocrine signals are involved in controlling quiescence, proliferation and differentiation of HSC with strong implications on expansion and differentiation ex vivo as well as in vivo transplantation. Towards this aim, a cell culture analysis on a polymer microcavity carrier platform was combined with a partial least square analysis of a mechanistic model of cell proliferation. We could demonstrate the discrimination of specific autocrine and paracrine signals from soluble factors as stimulating and inhibitory effectors in hematopoietic stem and progenitor cell culture. From that we hypothesize autocrine signals to be predominantly involved in maintaining the quiescent state of HSC in single-cell niches and advocate our analysis platform as an unprecedented option for untangling convoluted signaling mechanisms in complex cell systems being it of juxtacrine, paracrine or autocrine origin.

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mentioning

confidence: 99%

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Müller

Wang

Qiao

et al. 2016

Sci Rep

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“…Ultimately, the local microenvironment is responsible for directing hematopoietic response, and design of a culture system for HSC expansion and maintenance will be, by necessity, informed by in vivo cues. [30]…”

Section: Clinical Significance: Bottleneck In Expansionmentioning

confidence: 99%

Engineered Tissue Models to Replicate Dynamic Interactions within the Hematopoietic Stem Cell Niche

Gilchrist

Harley

2022

Adv Healthcare Materials

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Hematopoietic stem cells are the progenitors of the blood and immune system and represent the most widely used regenerative therapy. However, their rarity and limited donor base necessitate the design of ex vivo systems that support HSC expansion without the loss of long-term stem cell activity. This review describes recent advances in biomaterials systems to replicate features of the hematopoietic niche. Inspired by the native bone marrow, these instructive biomaterials provide stimuli and cues from cocultured niche-associated cells to support HSC encapsulation and expansion. Engineered systems increasingly enable study of the dynamic nature of the matrix and biomolecular environment as well as the role of cell-cell signaling (e.g., autocrine feedback vs paracrine signaling between dissimilar cells). The inherent coupling of material properties, biotransport of cell-secreted factors, and cell-mediated remodeling motivate dynamic biomaterial systems as well as characterization and modeling tools capable of evaluating a temporally evolving tissue microenvironment. Recent advances in HSC identification and tracking, model-based experimental design, and single-cell culture platforms facilitate the study of the effect of constellations of matrix, cell, and soluble factor signals on HSC fate. While inspired by the HSC niche, these tools are amenable to the broader stem cell engineering community.

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“…CSCs have been reported to overexpress ROS free-radical scavengers in order to reinforce their defense against ROS-induced damage [ 18 , 112 , 113 , 114 ], leading to tumor radioresistance. Compared to regular stem cells, CSCs are believed to reside predominantly in niches within the TME, including stromal cells, cancer-associated fibroblasts, infiltrating immune/inflammatory cells, and vascular endothelial cells [ 67 , 115 , 116 ], to retain their unique properties [ 117 , 118 ]. CSCs niche facilitates their metastatic potential and preserves their plasticity [ 22 , 23 ].…”

Section: Cancer Stem Cells Concept History and Propertiesmentioning

confidence: 99%

The Molecular and Cellular Strategies of Glioblastoma and Non-Small-Cell Lung Cancer Cells Conferring Radioresistance

Alhaddad

Osipov

Leonov

2022

IJMS

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Ionizing radiation (IR) has been shown to play a crucial role in the treatment of glioblastoma (GBM; grade IV) and non-small-cell lung cancer (NSCLC). Nevertheless, recent studies have indicated that radiotherapy can offer only palliation owing to the radioresistance of GBM and NSCLC. Therefore, delineating the major radioresistance mechanisms may provide novel therapeutic approaches to sensitize these diseases to IR and improve patient outcomes. This review provides insights into the molecular and cellular mechanisms underlying GBM and NSCLC radioresistance, where it sheds light on the role played by cancer stem cells (CSCs), as well as discusses comprehensively how the cellular dormancy/non-proliferating state and polyploidy impact on their survival and relapse post-IR exposure.

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Mimicking the Hematopoietic Stem Cell Niche by Biomaterials

Cited by 3 publications

References 103 publications

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Engineered Tissue Models to Replicate Dynamic Interactions within the Hematopoietic Stem Cell Niche

The Molecular and Cellular Strategies of Glioblastoma and Non-Small-Cell Lung Cancer Cells Conferring Radioresistance

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