3D chemical imaging of the brain using quantitative IR spectro-microscopy

Ogunleke, Abiodun; Recur, Benoît; Balacey, Hugo; Chen, Hsiang‐Hsin; Delugin, Maylis; Hwu, Y.; Javerzat, Sophie; Petibois, Cyril

doi:10.1039/c7sc03306k

Cited by 8 publications

(8 citation statements)

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“…SR-IR is 100-1000 times brighter than the traditional light source, enabling fourier transform infrared spectromicrotomography (FTIR-μCT) to reconstruct the image in the micron scale. By SR X-ray correction [42,43], IR was used to construct a 3D mapping of in-situ content of glucose, glycogen, and lactate in the brain of mice [44]. Transition metal homeostasis, such as copper, iron, and zinc, is an important property of brain and plays an important role in stroke and other brain diseases [45][46][47].…”

Section: Synchrotron Radiationmentioning

confidence: 99%

Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke

Zeng¹,

Chen²,

Yang³

et al. 2022

Int. J. Biol. Sci.

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As an important method to accurately and timely diagnose stroke and study physiological characteristics and pathological mechanism in it, imaging technology has gone through more than a century of iteration. The interaction of cells densely packed in the brain is three-dimensional (3D), but the flat images brought by traditional visualization methods show only a few cells and ignore connections outside the slices. The increased resolution allows for a more microscopic and underlying view. Today's intuitive 3D imagings of micron or even nanometer scale are showing its essentiality in stroke. In recent years, 3D imaging technology has gained rapid development. With the overhaul of imaging mediums and the innovation of imaging mode, the resolution has been significantly improved, endowing researchers with the capability of holistic observation of a large volume, real-time monitoring of tiny voxels, and quantitative measurement of spatial parameters. In this review, we will summarize the current methods of high-resolution 3D imaging applied in stroke.

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Section: Synchrotron Radiationmentioning

confidence: 99%

Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke

Zeng¹,

Chen²,

Yang³

et al. 2022

Int. J. Biol. Sci.

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“…Ogunleke et al demonstrated a high-throughput infrared microscopy method that utilized automated image correction followed by spectral analysis for 3D FTIR image reconstruction. The new approach enabled quantitative metabolic parameter extraction from the FTIR spectra for the characterization of the brain tumor metabolism [ 130 ]. Metasurface-enhanced infrared reflection spectroscopic cytopathology has been developed that utilizes plasmonic metasurfaces to localize and intensify the evanescent field near the cell’s membrane, and to conduct spectroscopic interrogations of the cells attached to the metasurface using reflected infrared light.…”

Section: Clinical Translation Of Ftirmentioning

confidence: 99%

Fourier Transform Infrared Spectroscopy in Oral Cancer Diagnosis

Wang

2021

IJMS

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Oral cancer is one of the most common cancers worldwide. Despite easy access to the oral cavity and significant advances in treatment, the morbidity and mortality rates for oral cancer patients are still very high, mainly due to late-stage diagnosis when treatment is less successful. Oral cancer has also been found to be the most expensive cancer to treat in the United States. Early diagnosis of oral cancer can significantly improve patient survival rate and reduce medical costs. There is an urgent unmet need for an accurate and sensitive molecular-based diagnostic tool for early oral cancer detection. Fourier transform infrared spectroscopy has gained increasing attention in cancer research due to its ability to elucidate qualitative and quantitative information of biochemical content and molecular-level structural changes in complex biological systems. The diagnosis of a disease is based on biochemical changes underlying the disease pathology rather than morphological changes of the tissue. It is a versatile method that can work with tissues, cells, or body fluids. In this review article, we aim to summarize the studies of infrared spectroscopy in oral cancer research and detection. It provides early evidence to support the potential application of infrared spectroscopy as a diagnostic tool for oral potentially malignant and malignant lesions. The challenges and opportunities in clinical translation are also discussed.

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“…10 To achieve 3D chemical imaging of a complete organ, a high throughput infrared microscopy method was established by combining Xray tomography and subsequent data analysis. 11 Moreover, optoacoustic imaging has been proven to be a new sensing method capable of deep penetration with a higher spatial resolution compared to uorescence imaging. In addition, a library screening approach was utilized to identify and evaluate the available dyes for multi-spectral optoacoustic tomography (MSOT) imaging.…”

mentioning

confidence: 99%