3D characterization of the vascular bed in bone metastasis of the rat by microcomputed tomography (microCT)

Nyangoga, Hervé; Libouban, Hélène; Basle, Michel‐Félix; Chappard, Daniel

doi:10.1016/j.bone.2011.03.615

Cited by 3 publications

(4 citation statements)

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“…Adequate filling of the arterial system was documented by distension of the visceral and body cavity vasculature by white silicone rubber solution. Previous studies have found that this observation corresponds to a physiologic pressure of 100 to 120 mm Hg (22). Mice were stored at 4 C overnight to allow the vascular contrast to polymerize, and then fixed in 4% PFA for 72 hours.…”

Section: Measures Of Cell Viability Proliferation and Apoptosismentioning

confidence: 99%

Autocrine VEGF/VEGFR1 Signaling in a Subpopulation of Cells Associates with Aggressive Osteosarcoma

Ohba

Cates

Cole³

et al. 2014

Molecular Cancer Research

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Section: Measures Of Cell Viability Proliferation and Apoptosismentioning

confidence: 99%

Autocrine VEGF/VEGFR1 Signaling in a Subpopulation of Cells Associates with Aggressive Osteosarcoma

Ohba

Cates

Cole³

et al. 2014

Molecular Cancer Research

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“…A small number of papers are concerned with localization of the vascular bed in different organs such as placenta, lung, bone, heart and prostate… [18][19][20][21]. The method has also been used to evaluate the vessels in different pathological conditions such as bone metastasis [22], diabetes [23], ischemia [24], cerebral angioma [25] or nerves [26].…”

Section: Several Techniques Have Been Proposed To Analyze Micro Vascu...mentioning

confidence: 99%

“…The most frequently used contrast agent is Microfil ® MV-122 (Flow Tech, Carver, MA), a silicon rubber compound developed to produce blood vessels casts and containing a colored mercury salt [22,23,[26][27][28]. Microfil ® is highly viscous and needs a decalcification of bone-containing sample that limits the possibility to analyze the precise relationships between the vascular network and bone.…”

Section: Several Techniques Have Been Proposed To Analyze Micro Vascu...mentioning

confidence: 99%

Microvascularization of the human central and peripheral nervous system: A new microcomputed tomography method

2020

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“…One of the practical limitations of this approach is the potential for Microfil to segregate and to shrink. [1][2][3] Segregation may occur if the Microfil components are not completely mixed, while shrinkage is particularly a problem if the tissue processing protocol involves exposure to solvents such as ethanol. 3 Thus, questions arise over the potential for volume changes in the Microfil between the time of infusion, its curing, and sample imaging.…”

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confidence: 99%

Micro‐computed tomographic analysis of the radial geometry of intrarenal artery‐vein pairs in rats and rabbits: Comparison with light microscopy

Ngo

Lê

Khan

et al. 2017

Clin Exp Pharma Physio

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We assessed the utility of synchrotron-radiation micro-computed tomography (micro-CT) for quantification of the radial geometry of the renal cortical vasculature. The kidneys of nine rats and six rabbits were perfusion fixed and the renal circulation filled with Microfil. In order to assess shrinkage of Microfil, rat kidneys were imaged at the Australian Synchrotron immediately upon tissue preparation and then post fixed in paraformaldehyde and reimaged 24 hours later. The Microfil shrank only 2-5% over the 24 hour period. All subsequent micro-CT imaging was completed within 24 hours of sample preparation. After micro-CT imaging, the kidneys were processed for histological analysis. In both rat and rabbit kidneys, vascular structures identified in histological sections could be identified in two-dimensional (2D) micro-CT images from the original kidney. Vascular morphology was similar in the two sets of images. Radial geometry quantified by manual analysis of 2D images from micro-CT was consistent with corresponding data generated by light microscopy. However, due to limited spatial resolution when imaging a whole organ using contrast-enhanced micro-CT, only arteries ≥100 and ≥60 μm in diameter, for the rat and rabbit respectively, could be assessed. We conclude that it is feasible and valid to use micro-CT to quantify vascular geometry of the renal cortical circulation in both the rat and rabbit. However, a combination of light microscopic and micro-CT approaches are required to evaluate the spatial relationships between intrarenal arteries and veins over an extensive range of vessel size.

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3D characterization of the vascular bed in bone metastasis of the rat by microcomputed tomography (microCT)

Cited by 3 publications

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Autocrine VEGF/VEGFR1 Signaling in a Subpopulation of Cells Associates with Aggressive Osteosarcoma

Autocrine VEGF/VEGFR1 Signaling in a Subpopulation of Cells Associates with Aggressive Osteosarcoma

Microvascularization of the human central and peripheral nervous system: A new microcomputed tomography method

Micro‐computed tomographic analysis of the radial geometry of intrarenal artery‐vein pairs in rats and rabbits: Comparison with light microscopy

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