3D cell culture opens new dimensions in cell-based assays

Justice, Bradley A.; Badr, Nadia; Felder, Robin A.

doi:10.1016/j.drudis.2008.11.006

Cited by 299 publications

(219 citation statements)

References 34 publications

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“…Damage to the ECM is known to adversely influence cell signalling pathways which affect important cell processes such as adhesion, proliferation, differentiation, migration, structure, and gene expression [4]. Furthermore, concerns exist with regards to the animal origin of some of the 3D ECM scaffolds and the associated production variability [1]. This, combined with the additional wash steps and extra handling requirements, results in high costs, well-to-well variations, and culture inconsistencies.…”

Section: Introductionmentioning

confidence: 99%

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Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Chetty

Vargha

Maity

et al. 2013

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Growing cells on 3D scaffolds is far superior to the conventional 2D monolayer culture method. In this study, a novel 3D thermoresponsive poly(propylene-g-Nisopropylacrylamide) (PP-g-PNIPAAm) non-woven fabric (gNWF) was developed for cell culture using oxyfluorination-assisted graft polymerisation (OAGP). New polar functional groups were detected on the oNWF, and PNIPAAm was confirmed in the gNWF by Attentuated Total-Reflectance Fourier transform infrared (ATR-FTIR) and scanning x-ray photoelectron spectroscopy (S-XPS). Scanning electron microscopy (SEM) revealed a rough surface morphology and confinement of the PNIPAAm graft layer to the surface of the fibres in the gNWF. The OAGP method did not affect the crystalline phase of bulk PP, however, twin-melting thermal peaks were detected for the oNWF and gNWF indicating crystal defects. Contact angle studies showed that the surface of the gNWF exhibited a thermoresponsive behaviour. Hepatocyte cells attached onto gNWF disks in a bioreactor at 37ºC and remained viable for 10 days in culture. Upon cooling the cell culture media to 20ºC, cells were spontaneously released as 3D multi-cellular constructs without requiring destructive enzymes. The development of 3D thermoresponsive scaffolds capable of non-invasive 3D cell culture could provide a more reliable in vitro model for cells.

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Section: Introductionmentioning

confidence: 99%

“…It is well-known that cells grown onto 3D surfaces show closer similarities to their in vivo counterparts in terms of cell morphologies, behaviour, and function than 2D surfaces [1,2]. A major limitation of existing 3D scaffolds however is that they rely on destructive means to release the proliferated cells.…”

Section: Introductionmentioning

confidence: 99%

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Chetty

Vargha

Maity

et al. 2013

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

show abstract

“…(2) The hanging-drop method involves hanging drops of cells in suspension [ 8 ]. (3) The use of scaffolds [ 9 ] or matrices [ 10 ] enable 3D formation while providing extracellular support. (4) Agitation-based approaches, which use rotary vessels to prevent cell adherence to the culture vessel due to its constant movement [ 11 ] or spinner fl asks with an internal stirring bar that keep cells and culture medium in constant, gentle motion [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Receptor Tyrosine Kinase Targeting in Multicellular Spheroids

Breslin

O’Driscoll

2014

Methods in Molecular Biology

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While growing cells as a monolayer is the traditional method for cell culture, the incorporation of multicellular spheroids into experimental design is becoming increasingly popular. This is due to the understanding that cells grown as spheroids tend to replicate the in vivo situation more reliably than monolayer cells. Thus, the use of multicellular spheroids may be more clinically relevant than monolayer cell cultures. Here, we describe methods for multicellular 3D spheroid generation that may be used to provide samples for receptor tyrosine kinase (and other protein) detection. Methods described include the forced-fl oating poly-HEMA method, the hanging-drop method, and the use of ECM to form multicellular 3D spheroids.

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“…Three-dimensional (3D) cell culture systems eliminate all these drawbacks of 2D cell culture systems, provide a high surface area for growth and migration, which can be tuned to support other cell behaviors, such as morphology, viability, proliferation, response to stimuli, differentiation or maturation and mimic human tissue micro-environment, pathological conditions and biological mechanisms much more closely [1,2]. 3D culture systems are used in an extensive area of cell-based studies [3], including cell adhesion/migration, tumour biology [4][5][6], stem cell research [7], regenerative medicine, tissue engineering and preclinical testing in drug discovery [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microtissue Forming Capacity of Sh-Sy5y and Sk-N-as Cell Lines

Taşdemir¹,

Ürkmez²,

Dogan³

2016

deufmd

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Two-dimensional (2D) cell culture systems are important tools for basic in vitro research. However, they form a thin monolayer structure and poorly mimic the complex in vivo conditions in terms of biochemical signals, cell-cell and cell-extracellular matrix (ECM) interactions. In this study, we performed a comparative study on SH-SY5Y and SK-N-AS neuroblastoma cell lines in terms of their microtissue forming capacity. Both cell lines are commonly used to model neurodegenerative diseases in vitro. Cells were cultured using 3D Petri Dish ® technique. The cells' microtissue forming capacity was observed morphologically and microtissues' size was analized. Results indicate that microtissue forming capacity of SH-SY5Ycell line was better than that of SK-N-AS cell line. SH-SY5Y microtissues can be used as an alternative, scaffold-free in vitro 3D model for neurodegenerative diseases and neuroblastoma research. Keywords: Cell culture, Three-dimensional (3D) Cell culture, Microtissue, SH-SY5Y, SK-N-A ÖZ

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3D cell culture opens new dimensions in cell-based assays

Cited by 299 publications

References 34 publications

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Receptor Tyrosine Kinase Targeting in Multicellular Spheroids

Comparison of Microtissue Forming Capacity of Sh-Sy5y and Sk-N-as Cell Lines

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