3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for bone tissue engineering

Murphy, Caroline A.; Kolan, Krishna C. R.; Li, Wenbin; Semon, Julie A.; Day, Delbert E.; Leu, Ming-Chuan

doi:10.18063/ijb.2017.01.005

Cited by 116 publications

(80 citation statements)

References 33 publications

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“…Briefly, the laser selectively sinters the powder layers under the control of the scanning system according to the cross-section profiles of the designed parts, forming the solid parts in a layer-by-layer manner [22,23] . The primary processing parameters, i.e., laser power, scanning speed, scanning spacing and layer thickness were set as 2 W, 200 mm/s, 0.1 mm and 0.1 mm, respectively.…”

Section: Scaffolds Preparationmentioning

confidence: 99%

An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

Shuai

Guo

Gao

et al. 2017

ijb

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Bone repair failure caused by implant-related infections is a common and troublesome problem. In this study, an antibacterial scaffold was developed via selective laser sintering with incorporating nano magnesium oxide (nMgO) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The results indicated the scaffold exerted high antibacterial activity. The antibacterial mechanism was that nMgO could cause oxidative damage and mechanical damage to bacteria through the production of reactive oxygen species (ROS) and direct contact action, respectively, which resulted in the damage of their structures and functions. Besides, nMgO significantly increased the compressive properties of the scaffold including strength and modulus, due to its excellent mechanical properties and uniform dispersion in the PHBV matrix. Moreover, the degradation tests indicated nMgO neutralized the acid degradation products of PHBV and benefited the degradation of the scaffold. The cell culture demonstrated that nMgO promoted the cellular adhesion and proliferation, as well as osteogenic differentiation. The present work may open the door to exploring nMgO as a promising antibacterial material for tissue engineering. Keywords: Nano magnesium oxide; antibacterial scaffolds; degradation properties; cytocompatibility; mechanical properties

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Section: Scaffolds Preparationmentioning

confidence: 99%

An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

Shuai

Guo

Gao

et al. 2017

ijb

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“…Hyaluronic acid was found to improve cell viability in bioprinted constructs . Methacrylated gelatin was found to enhance chondrogenesis …”

Section: Introductionmentioning

confidence: 99%

Photopolymerizable gelatin and hyaluronic acid for stereolithographic 3D bioprinting of tissue‐engineered cartilage

Lam¹,

et al. 2019

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To create artificial cartilage in vitro, mimicking the function of native extracellular matrix (ECM) and morphological cartilage‐like shape is essential. The interplay of cell patterning and matrix concentration has high impact on the phenotype and viability of the printed cells. To advance the capabilities of cartilage bioprinting, we investigated different ECMs to create an in vitro chondrocyte niche. Therefore, we used methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) in a stereolithographic bioprinting approach. Both materials have been shown to support cartilage ECM formation and recovery of chondrocyte phenotype. We used these materials as bioinks to create cartilage models with varying chondrocyte densities. The models maintained shape, viability, and homogenous cell distribution over 14 days in culture. Chondrogenic differentiation was demonstrated by cartilage‐typical proteoglycan and type II collagen deposition and gene expression (COL2A1, ACAN) after 14 days of culture. The differentiation pattern was influenced by cell density. A high cell density print (25 × 106 cells/mL) led to enhanced cartilage‐typical zonal segmentation compared to cultures with lower cell density (5 × 106 cells/mL). Compared to HAMA, GelMA resulted in a higher expression of COL1A1, typical for a more premature chondrocyte phenotype. Both bioinks are feasible for printing in vitro cartilage with varying differentiation patterns and ECM organization depending on starting cell density and chosen bioink. The presented technique could find application in the creation of cartilage models and in the treatment of articular cartilage defects using autologous material and adjusting the bioprinted constructs size and shape to the patient. © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2649–2657, 2019.

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“…PCL/bioactive borate glass was bioprinted with human adipose derived MSCs in the dimensions of 10 × 10 × 1 mm 3 and pore size in the range of 100–300 μm, using an extrusion‐based custom‐designed Cartesian 3D printer. In vitro and in vivo validation demonstrated highly vascularized bone constructs due to the presence of borate in the bioactive glass (Murphy et al, ).…”

Section: D Bioprintingmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for bone tissue engineering

Cited by 116 publications

References 33 publications

An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

Photopolymerizable gelatin and hyaluronic acid for stereolithographic 3D bioprinting of tissue‐engineered cartilage

Advances in three‐dimensional bioprinting of bone: Progress and challenges

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