3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels

Chen, You; Xiong, Xiong; Liu, Xin; Cui, Rongwei; Wang, Chen; Zhao, Guoru; Zhi, Wei; Lu, Mengjie; Duan, Ke; Weng, Jie; Qu, Shuxin; Ge, Jianhua

doi:10.1039/d0tb00060d

Cited by 78 publications

(59 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Biofabrication requires materials with properties that can both support cellular survival and growth and retain a designed 3D structure to result in a functioning living scaffold. Several studies have investigated the role of material properties that predict suitability for bioprinting, such as viscosity [ 174 , 175 , 176 , 177 , 178 ], shear-thinning [ 177 , 178 , 179 , 180 , 181 , 182 , 183 ], loss tangent [ 184 ], and, more recently, yield stress [ 184 , 185 ]. However, in the translation of synthetic peptide materials to bioinks, these key predictors are often under-reported and seemingly inconsistent.…”

Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

Enhancing Peptide Biomaterials for Biofabrication

et al. 2021

View full text Add to dashboard Cite

Biofabrication using well-matched cell/materials systems provides unprecedented opportunities for dealing with human health issues where disease or injury overtake the body’s native regenerative abilities. Such opportunities can be enhanced through the development of biomaterials with cues that appropriately influence embedded cells into forming functional tissues and organs. In this context, biomaterials’ reliance on rigid biofabrication techniques needs to support the incorporation of a hierarchical mimicry of local and bulk biological cues that mimic the key functional components of native extracellular matrix. Advances in synthetic self-assembling peptide biomaterials promise to produce reproducible mimics of tissue-specific structures and may go some way in overcoming batch inconsistency issues of naturally sourced materials. Recent work in this area has demonstrated biofabrication with self-assembling peptide biomaterials with unique biofabrication technologies to support structural fidelity upon 3D patterning. The use of synthetic self-assembling peptide biomaterials is a growing field that has demonstrated applicability in dermal, intestinal, muscle, cancer and stem cell tissue engineering.

show abstract

Section: Adapting Peptide Materials As Bioinksmentioning

confidence: 99%

Enhancing Peptide Biomaterials for Biofabrication

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Ideal bioinks experience shear-thinning and thixotropic properties, liquifying under shear-stresses to facilitate extrusion, and re- gelating quickly to maintain construct integrity. 61–64 Typically, extrusion-based printing favors high viscosity and thixotropy, while inkjet and light-based methods rely on low viscosity. 60 Mechanical strength can be improved through further cross-linking mechanisms (photopolymerization, 65,66 chemical, 67,68 and enzymatic 69,70 ) during or after printing.…”

Section: Printing and Seeding Constructsmentioning

confidence: 99%

“…We recommend that readers consider the following assessments when reporting extrusion printed 3D constructs: bioink quality (for fabrication), which may include filament and layering assessments, 51,53,71–73 and assessment of rheological measures of viscosity, 74 loss tangent, 75 yield stress, 76 and shear-thinning. 61–64 …”

Section: Printing and Seeding Constructsmentioning

confidence: 99%

Replace and repair: Biomimetic bioprinting for effective muscle engineering

Massey

Boyd‐Moss

et al. 2021

APL Bioengineering

View full text Add to dashboard Cite

The debilitating effects of muscle damage, either through ischemic injury or volumetric muscle loss (VML), can have significant impacts on patients, and yet there are few effective treatments. This challenge arises when function is degraded due to significant amounts of skeletal muscle loss, beyond the regenerative ability of endogenous repair mechanisms. Currently available surgical interventions for VML are quite invasive and cannot typically restore function adequately. In response to this, many new bioengineering studies implicate 3D bioprinting as a viable option. Bioprinting for VML repair includes three distinct phases: printing and seeding, growth and maturation, and implantation and application. Although this 3D bioprinting technology has existed for several decades, the advent of more advanced and novel printing techniques has brought us closer to clinical applications. Recent studies have overcome previous limitations in diffusion distance with novel microchannel construct architectures and improved myotubule alignment with highly biomimetic nanostructures. These structures may also enhance angiogenic and nervous ingrowth post-implantation, though further research to improve these parameters has been limited. Inclusion of neural cells has also shown to improve myoblast maturation and development of neuromuscular junctions, bringing us one step closer to functional, implantable skeletal muscle constructs. Given the current state of skeletal muscle 3D bioprinting, the most pressing future avenues of research include furthering our understanding of the physical and biochemical mechanisms of myotube development and expanding our control over macroscopic and microscopic construct structures. Further to this, current investigation needs to be expanded from immunocompromised rodent and murine myoblast models to more clinically applicable human cell lines as we move closer to viable therapeutic implementation.

show abstract

“…The results demonstrated that the osteogenic activity of SA/GG hydrogel was enhanced by the addition of nano TMP‐BG. [ 222 ] Also, the hybrid bioinks of nanocomposite laponite/gelatin, [ 223 ] HA/SA, [ 105 ] and α ‐TCP/alginate [ 224 ] were developed with improved osteogenic activity. However, these bioinks are usually formed into scaffolds by slow ionic crosslinking after printing, resulting in insufficient shape fidelity.…”

Section: Materials Used In Bone Reconstruction By Ammentioning

confidence: 99%

Recent Developments of Biomaterials for Additive Manufacturing of Bone Scaffolds

Zhang

et al. 2020

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Recent years have witnessed surging demand for bone repair/regeneration implants due to the increasing number of bone defects caused by trauma, cancer, infection, and arthritis worldwide. In addition to bone autografts and allografts, biomaterial substitutes have been widely used in clinical practice. Personalized implants with precise and personalized control of shape, porosity, composition, surface chemistry, and mechanical properties will greatly facilitate the regeneration of bone tissue and satiate the clinical needs. Additive manufacturing (AM) techniques, also known as 3D printing, are drawing fast growing attention in the fabrication of implants or scaffolding materials due to their capability of manufacturing complex and irregularly shaped scaffolds in repairing bone defects in clinical practice. This review aims to provide a comprehensive overview of recent progress in the development of materials and techniques used in the additive manufacturing of bone scaffolds. In addition, clinical application, pre-clinical trials and future prospects of AM based bone implants are also summarized and discussed.

show abstract

3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels

Abstract:
A novel shear-thinning hybrid bioink with good printability, mechanical support, biocompatibility, and bioactivity was developed by combining gellan gum, sodium alginate, and thixotropic magnesium phosphate-based gel (GG–SA/TMP-BG).

Cited by 78 publications

References 62 publications

Enhancing Peptide Biomaterials for Biofabrication

Enhancing Peptide Biomaterials for Biofabrication

Replace and repair: Biomimetic bioprinting for effective muscle engineering

Recent Developments of Biomaterials for Additive Manufacturing of Bone Scaffolds

Contact Info

Product

Resources

About

3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels

Abstract: A novel shear-thinning hybrid bioink with good printability, mechanical support, biocompatibility, and bioactivity was developed by combining gellan gum, sodium alginate, and thixotropic magnesium phosphate-based gel (GG–SA/TMP-BG).

Cited by 78 publications

References 62 publications

Enhancing Peptide Biomaterials for Biofabrication

Enhancing Peptide Biomaterials for Biofabrication

Replace and repair: Biomimetic bioprinting for effective muscle engineering

Recent Developments of Biomaterials for Additive Manufacturing of Bone Scaffolds

Contact Info

Product

Resources

About

Abstract:
A novel shear-thinning hybrid bioink with good printability, mechanical support, biocompatibility, and bioactivity was developed by combining gellan gum, sodium alginate, and thixotropic magnesium phosphate-based gel (GG–SA/TMP-BG).