3D bioprinting of proangiogenic constructs with induced immunomodulatory microenvironments through a dual cross-linking procedure using laponite incorporated bioink

Ma, Zhen; He, Hongtao; Deng, Changxu; Ren, Yanling; Lu, Dezhi; Li, Wentao; Sun, Xin; Wang, Wenhao; Zhang, Yuxin; Xu, Yuanjing; Zhou, Xiaojun; Zhou, Liang; Lin, Jun; Li, Tao; Wu, Tao; Wang, Jinwu

doi:10.1016/j.compositesb.2021.109399

Cited by 34 publications

(17 citation statements)

References 52 publications

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“…As the primary effector cells of nonspecific immunity, macrophages play a key role in orchestrating immunoinflammatory responses and tissue repair by releasing cytokines, growth factors, and chemokines to determine the fate of biomaterials [ 4 ]. In response to a variety of biophysical/biochemical signals, macrophages can be activated to a proinflammatory M1 phenotype, secreting inflammatory cytokines and participating as effector cells to resist intracellular pathogens, or an anti-inflammatory M2 phenotype, which has been found to take part in the alleviation of inflammation and the promotion of angiogenesis and tissue remodeling [ 56 ]. In particular, biomaterial-mediated regulation of macrophage polarization toward a balanced M1/M2 or predominantly M2 phenotype facilitated neovascularization, MSC recruitment, and osteogenesis, which could ensure successful long-term implantation in bone healing [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

Bioinspired sandwich-like hybrid surface functionalized scaffold capable of regulating osteogenesis, angiogenesis, and osteoclastogenesis for robust bone regeneration

Wu¹,

Chen²,

Liu³

et al. 2022

Materials Today Bio

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Section: Resultsmentioning

confidence: 99%

Bioinspired sandwich-like hybrid surface functionalized scaffold capable of regulating osteogenesis, angiogenesis, and osteoclastogenesis for robust bone regeneration

Wu¹,

Chen²,

Liu³

et al. 2022

Materials Today Bio

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“…However, linear PEG has certain limitations, such as low drug loading efficiency. In addition, micelles formed by linear PEG and other polymers easily collapse and lose their pharmacological effects; thus, it is difficult to meet the time required for bone defect repair ( Ma et al, 2022 ). A four-arm PEG increases the number of modified end groups, thereby increasing the drug loading and prolonging the degradation time in vivo .…”

Section: Fabrication Strategy Of Peg-based Hydrogelsmentioning

confidence: 99%

Drug delivery systems based on polyethylene glycol hydrogels for enhanced bone regeneration

Sun

Cui

Yuan

et al. 2023

Front. Bioeng. Biotechnol.

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Drug delivery systems composed of osteogenic substances and biological materials are of great significance in enhancing bone regeneration, and appropriate biological carriers are the cornerstone for their construction. Polyethylene glycol (PEG) is favored in bone tissue engineering due to its good biocompatibility and hydrophilicity. When combined with other substances, the physicochemical properties of PEG-based hydrogels fully meet the requirements of drug delivery carriers. Therefore, this paper reviews the application of PEG-based hydrogels in the treatment of bone defects. The advantages and disadvantages of PEG as a carrier are analyzed, and various modification methods of PEG hydrogels are summarized. On this basis, the application of PEG-based hydrogel drug delivery systems in promoting bone regeneration in recent years is summarized. Finally, the shortcomings and future developments of PEG-based hydrogel drug delivery systems are discussed. This review provides a theoretical basis and fabrication strategy for the application of PEG-based composite drug delivery systems in local bone defects.

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“…On the other hand, M2 macrophages secrete the highest levels of PDGF-BB, a chemoattractant stabilizing pericytes and promotes anastomosis of sprouting endothelial cells. 105,106 As a result, several researchers are increasingly utilizing such indirect modifications for their 3D printed constructs to promote neovascularization. 107,108 Zhou et al developed 3D printed silk scaffolds that were coated with PDA and were conjugated to copper peptide following PDA modification.…”

Section: Techniques To Promote Vascularization Inmentioning

confidence: 99%

“…For instance, primary human M1 macrophages secrete the highest levels of potent angiogenic stimulators, including VEGF. On the other hand, M2 macrophages secrete the highest levels of PDGF-BB, a chemoattractant stabilizing pericytes and promotes anastomosis of sprouting endothelial cells. , As a result, several researchers are increasingly utilizing such indirect modifications for their 3D printed constructs to promote neovascularization. , Zhou et al developed 3D printed silk scaffolds that were coated with PDA and were conjugated to copper peptide following PDA modification . In vitro studies demonstrated that sustained release of the copper peptides from the scaffolds induced M2 polarization of cultured macrophages.…”

Section: Techniques To Promote Vascularization In 3d Printed Scaffoldsmentioning

confidence: 99%

Strategies to Promote Vascularization in 3D Printed Tissue Scaffolds: Trends and Challenges

et al. 2022

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Three-dimensional (3D) printing techniques for scaffold fabrication have shown promising advancements in recent years owing to the ability of the latest high-performance printers to mimic the native tissue down to submicron scales. Nevertheless, host integration and performance of scaffolds in vivo have been severely limited owing to the lack of robust strategies to promote vascularization in 3D printed scaffolds. As a result, researchers over the past decade have been exploring strategies that can promote vascularization in 3D printed scaffolds toward enhancing scaffold functionality and ensuring host integration. Various emerging strategies to enhance vascularization in 3D printed scaffolds are discussed. These approaches include simple strategies such as the enhancement of vascular in-growth from the host upon implantation by scaffold modifications to complex approaches wherein scaffolds are fabricated with their own vasculature that can be directly anastomosed or microsurgically connected to the host vasculature, thereby ensuring optimal integration. The key differences among the techniques, their pros and cons, and the future opportunities for utilizing each technique are highlighted here. The Review concludes with the current limitations and future directions that can help 3D printing emerge as an effective biofabrication technique to realize tissues with physiologically relevant vasculatures to ultimately accelerate clinical translation.

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3D bioprinting of proangiogenic constructs with induced immunomodulatory microenvironments through a dual cross-linking procedure using laponite incorporated bioink

Cited by 34 publications

References 52 publications

Bioinspired sandwich-like hybrid surface functionalized scaffold capable of regulating osteogenesis, angiogenesis, and osteoclastogenesis for robust bone regeneration

Bioinspired sandwich-like hybrid surface functionalized scaffold capable of regulating osteogenesis, angiogenesis, and osteoclastogenesis for robust bone regeneration

Drug delivery systems based on polyethylene glycol hydrogels for enhanced bone regeneration

Strategies to Promote Vascularization in 3D Printed Tissue Scaffolds: Trends and Challenges

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