3D Bioprinting in Plant Science: An Interdisciplinary Approach

Mehrotra, Shakti; Kumar, Smita; Srivastava, Vikas; Mishra, Taijshee; Mishra, Bhartendu Nath

doi:10.1016/j.tplants.2019.10.014

Cited by 15 publications

(14 citation statements)

References 14 publications

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“…Our study provides the groundwork for using 3D bioprinting in the plant sciences field and details protocols and guidelines. In addition to the potential applications of 3D bioprinting for studying cell-to-cell communication and plant cell regeneration, 3D bioprinting can be used for researching cellular functions within a tunable environment, including the subcellular and molecular visualization of regulatory proteins, stress-responsive signaling, or cell wall dynamics ( 4 ). To enable these studies, the identification and detailed classification of the dimension parameters and signaling factors that stimulate plant cell processes, such as proliferation, cell attachment, regeneration, and differentiation, are crucial.…”

Section: Discussionmentioning

confidence: 99%

“…Multiple studies have established that conventional cell culturing methods do not entirely mimic the 3D microenvironment observed under natural conditions ( 2 , 3 ). 3D bioprinting enables the generation of specifically designed 3D cellular constructs that better simulate natural in planta conditions ( 4 ). 3D bioprinting can be performed through the application of one or multiple modalities for the precise spatial deposition of biologically active material, referred to as a bioink.…”

Section: Introductionmentioning

confidence: 99%

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Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting

et al. 2022

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Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted Arabidopsis root cells expressing endodermal markers was maintained for longer periods. The framework established here paves the way for a general use of 3D bioprinting for studying cellular reprogramming and cell cycle reentry toward tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting

et al. 2022

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“…3D scaffolds sustain improved cell proliferation and metabolic activity [104]. The ability to develop 3D structures supporting metabolically active cells permits the development of customisable culture systems [105]. However, before the nascent microalgae biocomposite field can afford serious consideration of 3D printed fabrication, we must advance the development of more affordable and easily accessible options.…”

Section: Bioinspiration From Lichenmentioning

confidence: 99%

Immobilising Microalgae and Cyanobacteria as Biocomposites: New Opportunities to Intensify Algae Biotechnology and Bioprocessing

et al. 2021

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There is a groundswell of interest in applying phototrophic microorganisms, specifically microalgae and cyanobacteria, for biotechnology and ecosystem service applications. However, there are inherent challenges associated with conventional routes to their deployment (using ponds, raceways and photobioreactors) which are synonymous with suspension cultivation techniques. Cultivation as biofilms partly ameliorates these issues; however, based on the principles of process intensification, by taking a step beyond biofilms and exploiting nature inspired artificial cell immobilisation, new opportunities become available, particularly for applications requiring extensive deployment periods (e.g., carbon capture and wastewater bioremediation). We explore the rationale for, and approaches to immobilised cultivation, in particular the application of latex-based polymer immobilisation as living biocomposites. We discuss how biocomposites can be optimised at the design stage based on mass transfer limitations. Finally, we predict that biocomposites will have a defining role in realising the deployment of metabolically engineered organisms for real world applications that may tip the balance of risk towards their environmental deployment.

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“…Despite cell totipotency, new plant cell-related discoveries in the field of bioprinting research are currently lacking. Moreover, the primary challenge to successful plant tissue printing is the lack of safe and intricate microarchitectures designed to mimic natural biological functions[ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…These constructs may aid in the resolution of unanswered biological questions, with potential for use as learning tools in plant research. Cellular components, such as the CW and mitochondria, are dynamic entities[ 19 ]; hence, understanding these dynamics is essential to determine the morphophysiological response of cells to various growth conditions. This can be easily accomplished using 3D bioprinting, while the layer-by-layer printing method can be useful for studying subcellular and molecular dynamics within plant cells.…”

Section: Introductionmentioning

confidence: 99%

A Review on Bioinks and their Application in Plant Bioprinting

Ghosh¹,

Yi²

2022

IJB

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In recent years, the characterization and fabrication methods concerning new bioinks have received much attention, largely because the absence of bioprintable materials has been identified as one of the most rudimentary challenges for rapid advancement in the field of three-dimensional (3D) printing. Bioinks for printing mammalian organs have been rapidly produced, but bioinks in the field of plant science remain sparse. Thus, 3D fabrication of plant parts is still in its infancy due to the lack of appropriate bioink materials, and aside from that, the difficulty in recreating sophisticated microarchitectures that accurately and safely mimic natural biological activities is a concern. Therefore, this review article is designed to emphasize the significance of bioinks and their applications in plant bioprinting.

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3D Bioprinting in Plant Science: An Interdisciplinary Approach

Cited by 15 publications

References 14 publications

Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting

Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting

Immobilising Microalgae and Cyanobacteria as Biocomposites: New Opportunities to Intensify Algae Biotechnology and Bioprocessing

A Review on Bioinks and their Application in Plant Bioprinting

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