3D Bioprinting: 3D Bioprinting Using a Templated Porous Bioink (Adv. Healthcare Mater. 14/2016)

Armstrong, James R.; Burke, Madeline; Carter, Benjamin M.; Davis, Sean A.; Perriman, Adam W.

doi:10.1002/adhm.201670070

Cited by 5 publications

(7 citation statements)

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“…Due to their potential, BM-MSCs could engineer different layers of native cartilage in vitro. MSCs are usually embedded in a hydrogel to reproduce the hyaline-like cartilaginous matrix, due to their excellent hydration properties, such as alginate [162] GelMA [163] or dECM-based bioinks [106]. An important aspect of tissue mimetism is the fiber organization within the different layers; the bioextrusion process can print layers with different alignments, making it possible to reproduce the collagen fibers' natural organization within the cartilaginous ECM [103].…”

Section: Mature Mscsmentioning

confidence: 99%

“…Very few studies used low cell densities, but they achieved good chondrogenic results [154,158]. For BM-MSCs, the range of densities is comparable to that of chondrocytes, starting from 4 × 10 6 cells/mL [175] and increasing to as high as 20 × 10 6 cells/mL [163]; most of the studies were between those two values, thus achieving the best chondrogenic induction possible [106,162,165,166]. Recently, some research aimed to compare two very low cell densities, 1 and 2 × 10 6 cells/mL, to assess better options for obtaining good chondrogenesis, and they showed that the lowest density seemed to be optimal [60].…”

Section: Cell Density For Cartilage Tissue 3d Printingmentioning

confidence: 99%

“…Other printing parameters, such as cross-linking (UV light, ionic, or enzymatic gelation), also need to be tuned to permit maximum cell survival [60,103,119,165,166]. To assess the long-term effects of the overall printing process, many researchers evaluated viability at different times postprinting [162,165,166]. The biomaterials and additives in the bioink can also affect cell viability, exerting a cytotoxic effect on the embedded cells.…”

Section: Cell Viabilitymentioning

confidence: 99%

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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

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Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

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Section: Mature Mscsmentioning

confidence: 99%

Section: Cell Density For Cartilage Tissue 3d Printingmentioning

confidence: 99%

Section: Cell Viabilitymentioning

confidence: 99%

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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

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“…Nanomaterial science is expected to enhance the performance of 3D printed devices to unprecedented standards. The chemistry of the inks influences biological signal transduction and that suitable for biosensing needs to meet criteria of biocompatibility, specific affinity, and a processing flow matching a particular viscosity range [ 96 ].…”

Section: Future Directions For Graphene-based Biosensors For Potenmentioning

confidence: 99%

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Amărandi

Becheru

Vlăsceanu

et al. 2018

BioMed Research International

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Regenerative medicine is challenged by the need to conform to rigorous guidelines for establishing safe and effective development and translation of stem cell-based therapies. Counteracting widespread concerns regarding unproven cell therapies, stringent cell-based assays seek not only to avoid harm but also to enhance quality and efficacy. Potency indicates that the cells are functionally fit for purpose before they are administered to the patient. It is a paramount quantitative critical quality attribute serving as a decisive release criterion. Given a broad range of stem cell types and therapeutic contexts the potency assay often comprises one of the most demanding hurdles for release of a cell therapy medicinal product. With need for improved biomarker assessment and expedited measurement, recent advances in graphene-based biosensors suggest that they are poised to be valuable platforms for accelerating potency assay development. Among several potential advantages, they offer versatility for sensitive measurement of a broad range of potential biomarker types, cell biocompatibility for direct measurement, and small sample sufficiency, plus ease of use and point-of-care applicability.

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“…Despite the simplicity of this approach, poor printing resolution and construct heterogeneity resulting from insufficient control over gelation time, as well as the cellular toxicity of crosslinking agents, have fostered the development of novel hybrid multicomponent bioinks in which pre-crosslinking is no longer needed. The combination of alginate with other natural and synthetic materials such as cellulose [18][19][20], gelatin [9,21,22], hyaluronic acid [23] and Pluronic F-127 [24], among other thickening agents, has resulted in bioinks with optimal viscosity and high cell viability post-printing.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Alginate-Based Bioinks for 3D Bioprinting, Mesenchymal Stromal Cell Osteogenesis, and Application for Patient-Specific Bone Grafts

Fernández

Tenorio

Campbell

et al. 2020

Preprint

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To realize the promise of 3D bioprinting, it is imperative to develop bioinks that possess the biological and rheological characteristics needed for the printing of cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through pre-crosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physicochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl2 (alg-CaCl2), alginate-CaSO4 (alg-CaSO4), alginate-gelatin (alg-gel) and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of patient-specific osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the pre-crosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO4 and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl2 or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO4 and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days post-printing. In addition, effective cell-matrix interactions were only observed in alg-CaCl2 printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl2 constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of in vitro culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues.

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3D Bioprinting: 3D Bioprinting Using a Templated Porous Bioink (Adv. Healthcare Mater. 14/2016)

Cited by 5 publications

References 0 publications

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Evaluation of Alginate-Based Bioinks for 3D Bioprinting, Mesenchymal Stromal Cell Osteogenesis, and Application for Patient-Specific Bone Grafts

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