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Bolte, Gabriele; Knauss, Margarete; Metzdorf, Irene; Stern, Martin

doi:10.1023/a:1018844608861

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…Since paracellular transport is basically size-dependent, the permeation rate of the two proteins are similar if paracellular transport is the major pathway. A possible reason for the different permeation rates and behaviors of the two proteins is the protein binding capacities of the brush border membrane, as suggested by Bolte et al 16,17) As the concentration of IL-4, 10 ng/ml of IL-4 was used to stimulate T84 cells in this study. The serum concentration of atopic patients ranges from 231 to 2,900 pg/ml.…”

Section: Discussionmentioning

confidence: 99%

Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers

Mochizuki

Satsu

Totsuka

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 C. The permeability of T84 cell monolayers to -lactogulobulin (-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN 3 ), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4.

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Section: Discussionmentioning

confidence: 99%

Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers

Mochizuki

Satsu

Totsuka

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…Изменения созревания также влияют на мембраны эпителиальных клеток, которые являются главным механическим интерфейсом между внутренней средой хозяина и содержимым просвета. Постнатальное созревание щеточной каймы тонкой кишки связано с повышенной способностью объединять пищевые белки [4]. Способность антигенов связываться с эпителиальными клетками сопряжена со скоростью и маршрутом переноса антигенов и, как было показано, влияет на интенсивность иммунных ответов слизистых оболочек [5].…”

Section: Introductionunclassified

Immunomodulatory effects of probiotics

Plotnikova

Zakharova

2020

Medicinskij sovet

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Today, there is an explosion of discoveries related to a growing understanding of the role of microbial communities, key species of bacteria, products or metabolites derived from commensals, and in particular the relationship between some of these components and painful conditions in humans. Microbiota plays a fundamental role in the induction, training and functioning of the host’s immune system. In turn, the immune system has evolved significantly as a means of maintaining the host’s symbiotic relations with these very diverse and developing microbes. At optimal performance, this alliance of the immune system and microbiota allows to induce protective reactions to pathogens and maintain regulatory pathways involved in maintaining tolerance to harmless antigens. In this review, we outline the role of the intestinal microbiota in the immune system, starting with initial information supporting further insights into the effects of intestinal microbiota dysbacteriosis on the host’s susceptibility to infection. Probiotics are considered a good form of therapy to control harmful intestinal microorganisms, improve digestion and the absorption of nutrients. The beneficial effects of probiotics have been demonstrated in many diseases. One of the main mechanisms of probiotics is the regulation of the immune response of the host. The article reviews and discusses the regulatory role of probiotics in the “intestine-lungs” system and the immune system of mucous membranes for potential antiviral mechanisms. The unique role of probiotics in modulation of intestinal microbes and establishment of intestinal homeostasis and production of interferon as antiviral mechanism is described. In addition, the regulatory role of probiotics in the system «intestine-lungs» and the immune system of the mucous membranes for potential antiviral mechanisms, including in COVID-19 is considered and discussed. Symbiotic products Bac-Set® Forte and Bac-Set® Cold/Flu are described.

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Horseradish peroxidase binding to intestinal brush-border membranes ofCyprinus carpio. Identification of a putative receptor

et al. 2000

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Morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (HRP) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. The aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (BBM) to demonstrate and characterize these specific HRP binding sites. The results obtained show that HRP binding to BBM exhibits a saturable mode and high affinity (K(d) = 22 nM). In addition, HRP binding sites are highly enriched in BBM compared to basolateral membranes. On the other hand, HRP interaction with these sites is apparently of an ionic character because binding increased concomitantly with decreasing NaCl concentrations in the assay, reaching a maximum in the absence of NaCl. Other proteins that are also internalized in carp intestine did not significantly inhibit HRP binding to BBM. A lectin-type of interaction was discarded because neither manan nor ovoalbumin inhibited HRP binding. Proteinase K treatment of BBM reduced HRP binding by 70%, suggesting a proteic nature for this binding site. Finally, ligand blotting assays showed that HRP binds specifically to a 15.3-kDa protein. Taken together, these results are consistent with the existence of a functional receptor for HRP in carp intestinal mucosa that could mediate its internalization.

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Untitled

Cited by 7 publications

References 43 publications

Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers

Transepithelial Transport of Macromolecular Substances in IL-4 Treated Human Intestinal T84 Cell Monolayers

Immunomodulatory effects of probiotics

Horseradish peroxidase binding to intestinal brush-border membranes ofCyprinus carpio. Identification of a putative receptor

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