366 POSTER Mechanism of the initiation of DNA methylation de novo by small RNA

Halytskiy, V.

doi:10.1016/s1359-6349(07)70384-1

Cited by 3 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, we found in silico that the con centration of CG dinucleotide amounts to 2.85% in siRNA sequences and to 2.58 ± 0.17% in mature miRNA sequences [21][22][23]. It was quite intriguing that the concentration of CG sites in miRNAs and siRNAs significantly exceeds the general genome level.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…The frequency of CNG trinucleotides (here N designates any nucleotide) amounts to 6.29% in siRNA sequences and to 5.77 ± 0.25% in mature miRNA sequences [21][22][23] as well as thymine. Thus, CNG trinucleotide was also found significantly more often in siRNA and miRNA sequences than in the random genome se quence.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…on this basis, we had proposed that siR NAs and miRNAs should directly scan the DNA strands unwinding during transcription (this ex plains dependence of RNAmediated transcrip tional silencing on functioning of RNA polymera se) [21,22,24]. Most likely, interfering RNAs interact with the nontemplate (coding) DNA strand.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…This should involve the cellular DNA methyltransferases in cytosine methylation de novo in CG dinucleotides and CNG trinucleotides, which are bound to complementary sites in the interfering RNA ( fig. 1) [22]. The maintenance methyltransferase DNMT1 can methylate the com plementary DNA strand (template) already after the removal of interfering RNA from RNADNA duplex and renewal of the doublestranded DNA structure.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

See 3 more Smart Citations

Specific silencing of leukemic oncogenes using RNA-interference approach

Halytskiy¹,

Комісаренко²

2013

Ukr.Biochem.J.

View full text Add to dashboard Cite

Non-coding rNAs are widespread class of cell rNAs. they participate in many important processes in cells-signaling, posttranscriptional silencing, protein biosynthesis, splicing, maintenance of genome stability, telomere lengthening, X-inactivation. Nevertheless, activity of these rNAs is not restricted to posttranscriptional sphere, but cover also processes that change or maintain the epigenetic information. Non-coding rNAs can directly bind to the DNA targets and cause their repression through recruitment of DNA methyltransferases as well as chromatin modifying enzymes. Such events constitute molecular mechanism of the rNA-dependent DNA methylation. It is possible, that the rNA-DNA interaction is universal mechanism triggering DNA methylation de novo. Allelic exclusion can be also based on described mechanism. this phenomenon takes place, when non-coding rNA, which precursor is transcribed from one allele, triggers DNA methylation in all other alleles present in the cell. Note, that mirNA-mediated transcriptional silencing resembles allelic exclusion, because both mirNA gene and genes, which can be targeted by this mirNA, contain elements with the same sequences. It can be assumed that rNA-dependent DNA methylation and allelic exclusion originated with the purpose of counteracting the activity of mobile genetic elements. Probably, thinning and deregulation of the cellular non-coding rNA pattern allows reactivation of silent mobile genetic elements resulting in genome instability that leads to ageing and carcinogenesis. In the course of X-inactivation, DNA methylation and subsequent hete rochromatinization of X chromosome can be triggered by direct hybridization of 5′-end of large non-coding rNA Xist with DNA targets in remote regions of the X chromosome.

show abstract

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

See 2 more Smart Citations

Specific silencing of leukemic oncogenes using RNA-interference approach

Halytskiy¹,

Комісаренко²

2013

Ukr.Biochem.J.

View full text Add to dashboard Cite

show abstract

“…Îäíèì ³ç àâ òîð³â äà íî¿ ñòàòò³ íå ùî äàâ íî çà ïðîïî íî âà íî ìå õàí³çì RNAi-îïî ñå ðåä êî âà íî ãî òðàíñ -êðèïö³éíî ãî ñàé ëåí ñèí ãó [30,31], ÿêèé ïî ëÿ ãàº â òî ìó, ùî RNAi ïðî íè êàº ó êë³òèí íå ÿä ðî, äå ñêà íóº íóê ëå î òèä íó ïî ñë³äîâí³ñòü ëàí öþã³â ÄÍÊ ó ì³ðó ¿õíüî ãî ðîçïë³òàí íÿ ÐÍÊ-ïîë³ìå ðà çîþ II ïðè òðàíñ êðèïö³¿. Âè ÿ âèâ øè êîì ïëå ìåí òàð íó ä³ëÿí êó ÄÍÊ, RNAi çâ'ÿ çóºòüñÿ ç íåþ ³ ðåê ðó òóº êë³òèí íó ÄÍÊ-ìå òèë òðàí ñôå ðà çó DNMT3, ÿêà ìå òè ëþº â ÄÍÊ de novo öè òî çèí äè íóê ëå î òèä³â 5'-CG-3' òà òðè íóê ëå î òèä³â 5'-CNG-3', ñïàðåíèõ ç àíà ëîã³÷íèìè ñàéòàìè ó ñêëàä³ RNAi [30].…”

unclassified

Recombination in immunoglobulin gene loci

Halytskiy

Комісаренко

2009

Biopolym. Cell

View full text Add to dashboard Cite

show abstract

5'-CG-3', 5'-CNG-3', 5'-GC-3' and 5'-GNC-3' site localization in microRNA sequences

Halytskiy

Комісаренко

2011

Biopolym. Cell

View full text Add to dashboard Cite

Aim. Discovery of localization pattern of 5'-CG-3' dinucleotide and 5'-CNG-3' trinucleotide in microRNA (miRNA) sequences. Methods. Bioinformatic and statistic analysis of mature miRNA sequences. Results. One of the highest peaks of 5'-CG-3' dinucleotides concentration is located close to the nucleotide 10 from 5'-end of miRNA. The level of 5'-CG-3' in this peak exceeds more than four times the average genomic frequency of this dinucleotide. Other evolutionary conservative peaks are located around the nucleotides 16 and 21 in miRNA sequence. Peaks of 5'-CNG-3' trinucleotides concentration correspond to the nucleotides 4 and 8. Approximately 50 % of miRNA sequences contain two or more 5'-CG-3' or 5'CNG-3' sites. The distance between these sites amounts mostly to 1 or 3–9 nucleotides. Conclusions. The peaks of 5'-CG-3' and 5'CNG-3' sites concentration are located not only within seed region, but also along the full length of miRNA sequence. Nevertheless, these sites are disposed in the same miRNA molecule mainly within one turn of double helix. Such site localization pattern may be an evidence of participation of the whole miRNA sequence in miRNAdependent DNA methylation

show abstract

366 POSTER Mechanism of the initiation of DNA methylation de novo by small RNA

Cited by 3 publications

References 0 publications

Specific silencing of leukemic oncogenes using RNA-interference approach

Specific silencing of leukemic oncogenes using RNA-interference approach

Recombination in immunoglobulin gene loci

5'-CG-3', 5'-CNG-3', 5'-GC-3' and 5'-GNC-3' site localization in microRNA sequences

Contact Info

Product

Resources

About