Recombination in immunoglobulin gene loci

Halytskiy, V.; Комісаренко, С. В.

doi:10.7124/bc.0007c6

Cited by 1 publication

(8 citation statements)

References 36 publications

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“…In miRNAs, one of the greatest peaks of CG site concentration is located close to the nucleotide 10 from 5′end [23]. The level of CG sites exceeds in this peak more than four the average genomic frequency of this dinucleotide.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 96%

“…Nevertheless, we found in silico that the con centration of CG dinucleotide amounts to 2.85% in siRNA sequences and to 2.58 ± 0.17% in mature miRNA sequences [21][22][23]. It was quite intriguing that the concentration of CG sites in miRNAs and siRNAs significantly exceeds the general genome level.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…The frequency of CNG trinucleotides (here N designates any nucleotide) amounts to 6.29% in siRNA sequences and to 5.77 ± 0.25% in mature miRNA sequences [21][22][23] as well as thymine. Thus, CNG trinucleotide was also found significantly more often in siRNA and miRNA sequences than in the random genome se quence.…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…It is also very significant that only 15.69% siRNA sequences and 20.01% mature miRNA se quences contain none of CG or CNG sites [23,24].…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…other evolutionary conservative peaks are located around the nucle otides 16 and 21 in miRNA sequence. Peaks of CNG site concentration correspond with nucleo tides 4 and 8 [23].…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

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Specific silencing of leukemic oncogenes using RNA-interference approach

Halytskiy¹,

Комісаренко²

2013

Ukr.Biochem.J.

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Non-coding rNAs are widespread class of cell rNAs. they participate in many important processes in cells-signaling, posttranscriptional silencing, protein biosynthesis, splicing, maintenance of genome stability, telomere lengthening, X-inactivation. Nevertheless, activity of these rNAs is not restricted to posttranscriptional sphere, but cover also processes that change or maintain the epigenetic information. Non-coding rNAs can directly bind to the DNA targets and cause their repression through recruitment of DNA methyltransferases as well as chromatin modifying enzymes. Such events constitute molecular mechanism of the rNA-dependent DNA methylation. It is possible, that the rNA-DNA interaction is universal mechanism triggering DNA methylation de novo. Allelic exclusion can be also based on described mechanism. this phenomenon takes place, when non-coding rNA, which precursor is transcribed from one allele, triggers DNA methylation in all other alleles present in the cell. Note, that mirNA-mediated transcriptional silencing resembles allelic exclusion, because both mirNA gene and genes, which can be targeted by this mirNA, contain elements with the same sequences. It can be assumed that rNA-dependent DNA methylation and allelic exclusion originated with the purpose of counteracting the activity of mobile genetic elements. Probably, thinning and deregulation of the cellular non-coding rNA pattern allows reactivation of silent mobile genetic elements resulting in genome instability that leads to ageing and carcinogenesis. In the course of X-inactivation, DNA methylation and subsequent hete rochromatinization of X chromosome can be triggered by direct hybridization of 5′-end of large non-coding rNA Xist with DNA targets in remote regions of the X chromosome.

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Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 96%

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…It is also very significant that only 15.69% siRNA sequences and 20.01% mature miRNA se quences contain none of CG or CNG sites [23,24].…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

“…other evolutionary conservative peaks are located around the nucle otides 16 and 21 in miRNA sequence. Peaks of CNG site concentration correspond with nucleo tides 4 and 8 [23].…”

Section: Concentration Of C(n)g Sites In Sirnas and Mirnasmentioning

confidence: 99%

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