Development of 6E3 antibody-mediated SERS immunoassay for drug-resistant influenza virus

Kim, Hye‐Ran; Kang, Hyunju; Kim, Hye Nan; Kim, Hongki; Moon, Jeong; Guk, Kyeonghye; Park, Hwangseo; Yong, Dongeun; Bae, Pan Kee; Park, Hyun Gyu; Lim, Eun‐Kyung; Kang, Taejoon; Jung, Juyeon

doi:10.1016/j.bios.2021.113324

Cited by 21 publications

(12 citation statements)

References 40 publications

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“…COVID-19 is predicted to become an endemic disease ( Torjesen, 2021 ; Veldhoen and Simas, 2021 ); therefore, the development of multiplex POC devices is crucial to distinguish different respiratory illnesses in the future ( Veldhoen and Simas, 2021 ). Our group has been involved in successfully developing various types of antibodies for the detection of viruses to date ( Kim et al, 2021 ; Orooji et al, 2021 ). Thus, in the near future, we plan to combine such high-performance antibodies to develop a multiplex immunoassay for infectious viruses.…”

Section: Discussionmentioning

confidence: 99%

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cha

Kim

Joung

et al. 2022

Biosensors and Bioelectronics

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

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Section: Discussionmentioning

confidence: 99%

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cha

Kim

Joung

et al. 2022

Biosensors and Bioelectronics

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“…SERS-based sensing technology mainly depends on the performance of nano SERS-active substrate, so the properties of nanomaterials, such as high surface energy, agglomeration and dispersion, surface plasmon resonance, and the preparation technology of nanomaterials will have influence on the activity of SERS substrate [8,9]. In medical detection, SERS-based sensing technology has been widely applied to cancer detection, virus detection, biological imaging, and other medical fields [10][11][12]. Limited by the inherent non-specificity of SERS substrate itself, SERS-based biosensors need to be modified by biomolecules such as proteins, antibodies, and aptamers on the surface of the SERS-active substrate to specifically capture targets to be detected [13,14].…”

Section: Sers-based Biosensorsmentioning

confidence: 99%

Perspective Chapter: Novel Diagnostics Methods for SARS-CoV-2

Yang¹,

Li²

2023

Infectious Diseases

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence has given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests such as PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the various novel diagnostics methods including SERS, SPR, electrochemical, magnetic detection of SARS-CoV-2. All sensing and biosensing methods based on nanotechnology developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system.

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“…The enhancement mechanism of SERS consists mainly of electromagnetic enhancement and chemical enhancement, which are caused by the surface plasmon resonance of the substrate and the charge transfer between the substrate and the probe molecules, respectively [ 71 , 72 ]. Surface-enhanced Raman detection technology has a high sensitivity, and the detection ability of some SERS-active substrates can even reach the single molecule, which has been widely used in cancer detection, virus detection, biological imaging, and other medical fields [ 73 , 74 , 75 , 76 ]. Recently, SERS has gained much attention in the rapid detection of SARS-CoV-2 and the infectivity judgment of SARS-CoV-2 fields due to its high sensitivity, accuracy, rapidity, and low cost.…”

Section: Sers Biosensorsmentioning

confidence: 99%

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2

Lin

et al. 2022

Biosensors

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The outbreak of Corona Virus Disease 2019 (COVID-19) has again emphasized the significance of developing rapid and highly sensitive testing tools for quickly identifying infected patients. Although the current reverse transcription polymerase chain reaction (RT-PCR) diagnostic techniques can satisfy the required sensitivity and specificity, the inherent disadvantages with time-consuming, sophisticated equipment and professional operators limit its application scopes. Compared with traditional detection techniques, optical biosensors based on nanomaterials/nanostructures have received much interest in the detection of SARS-CoV-2 due to the high sensitivity, high accuracy, and fast response. In this review, the research progress on optical biosensors in SARS-CoV-2 diagnosis, including fluorescence biosensors, colorimetric biosensors, Surface Enhancement Raman Scattering (SERS) biosensors, and Surface Plasmon Resonance (SPR) biosensors, was comprehensively summarized. Further, promising strategies to improve optical biosensors are also explained. Optical biosensors can not only realize the rapid detection of SARS-CoV-2 but also be applied to judge the infectiousness of the virus and guide the choice of SARS-CoV-2 vaccines, showing enormous potential to become point-of-care detection tools for the timely control of the pandemic.

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Development of 6E3 antibody-mediated SERS immunoassay for drug-resistant influenza virus

Cited by 21 publications

References 40 publications

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Perspective Chapter: Novel Diagnostics Methods for SARS-CoV-2

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2

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