Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cha, Hyunjung; Kim, Hye‐Ran; Joung, Younju; Kang, Hyunju; Moon, Jeong; Jang, Hyowon; Park, Sohyun; Kwon, Hyung Jun; Lee, In-Chul; Kim, Sun‐Joo; Yong, Dongeun; Yoon, Sun‐Woo; Park, Sung‐Gyu; Guk, Kyeonghye; Lim, Eun‐Kyung; Park, Hyun Gyu; Choo, Jaebum; Jung, Juyeon; Kang, Taejoon

doi:10.1016/j.bios.2022.114008

Cited by 42 publications

(24 citation statements)

References 66 publications

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“…In this study, we developed a high-sensitivity surface-enhanced Raman scattering (SERS)-LFA system by combining an SERS-based LFA strip with a portable Raman spectrophotometer to solve the sensitivity problem of the commercial LFA strip. Recently, several studies on SERS detection using SARS-CoV-2 have been published. − In addition, several custom-built SERS readers that can rapidly diagnose clinical samples in the field have also been reported. − Nevertheless, a diagnostic system that can detect SARS-CoV-2 antigen with high sensitivity in the field has not yet been reported. Herein, we manufactured a portable SERS-LFA reader that is capable of analyzing SARS-CoV-2 on-site and determined the calibration curve and LoD along with the SARS-CoV-2 concentration.…”

mentioning

confidence: 99%

Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader

Joung

Kım

Lee³

et al. 2022

ACS Sens.

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In early 2022, the number of people infected with the highly contagious mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), called Omicron, was increasing worldwide. Therefore, several countries approved the lateral flow assay (LFA) strip as a diagnostic method for confirming SARS-CoV-2 instead of reverse transcription-polymerase chain reaction (RT-PCR), which takes a long time to generate the results. However, owing to the limitation of detection sensitivity, commercial LFA strips have high false-negative diagnosis rates for patients with low virus concentrations. Therefore, in this study, we developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to solve the sensitivity problem of the commercial LFA strip. We tested 54 clinical samples using this portable SERS-LFA reader, which generated 49 positive and 5 negative results. Out of the 49 positive results, SERS-LFA classified only 2 as false negative, while the commercial LFA classified 21 as false negative. This confirmed that the false-negative rate had significantly improved compared to that of commercial LFA strips. We believe that the proposed SERS-LFA system can be utilized as a point-of-care diagnostic system to quickly and accurately determine a virus infection that could spread significantly within a short period.

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confidence: 99%

Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader

Joung

Kım

Lee³

et al. 2022

ACS Sens.

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“…The strains were cultured as described previously. 52 , 53 All experiments involving the use of SARS-CoV-2 were performed at the Korea Centers for Disease Control and Prevention (KCDC)-approved BL-3 facility of KRIBB in accordance with institutional biosafety requirements. The genomic RNA of the viruses (SARS-CoV-2, influenza A virus subtype H1N1, H3N2, H5N2, H1N2, and H3H8, and RSV A) was provided by the NCCP.…”

Section: Methodsmentioning

confidence: 99%

“…SARS-CoV-2 strains (BetaCoV/Korea/KCDC03/2020, hCoV-19/Korea/KDCA51463/2021; GR, B.1.1.7, hCoV-19/Korea/KDCA55905/2021; GH, B.1.351, hCoV-19/Korea/KDCA72731/2021; GR, P.2 lineage, hCoV-19/Korea/KDCA49671/2021; GH, B.1.427, hCoV-19/Korea/KDCA59777/2021; GH, B.1.429, hCoV-19/Korea/KDCA79765/2021; G, B.1.525, hCoV-19/Korea/KDCA49671/2021; GH, B.1.526, hCoV-19/Korea/KDCA95637/2021; GR, P.1, hCoV-19/Korea/KDCA105288/2021; G, B.1.617.1, hCoV-19/Korea/KDCA119861/2021; G, B.1.617.2, hCoV-19/Korea/KDCA447321/2021; GRA, B.1.1529) were provided by the National Culture Collection for Pathogens (NCCP), which is operated by the Korea National Institute of Health. The strains were cultured as described previously. , All experiments involving the use of SARS-CoV-2 were performed at the Korea Centers for Disease Control and Prevention (KCDC)-approved BL-3 facility of KRIBB in accordance with institutional biosafety requirements. The genomic RNA of the viruses (SARS-CoV-2, influenza A virus subtype H1N1, H3N2, H5N2, H1N2, and H3H8, and RSV A) was provided by the NCCP.…”

Section: Methodsmentioning

confidence: 99%

Smartphone-Based SARS-CoV-2 and Variants Detection System using Colorimetric DNAzyme Reaction Triggered by Loop-Mediated Isothermal Amplification (LAMP) with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)

et al. 2022

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Coronavirus disease (COVID-19) has affected people for over two years. Moreover, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has raised concerns regarding its accurate diagnosis. Here, we report a colorimetric DNAzyme reaction triggered by loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR), referred to as DAMPR assay for detecting SARS-CoV-2 and variants genes with attomolar sensitivity within an hour. The CRISPR-associated protein 9 (Cas9) system eliminated false-positive signals of LAMP products, improving the accuracy of DAMPR assay. Further, we fabricated a portable DAMPR assay system using a three-dimensional printing technique and developed a machine learning (ML)-based smartphone application to routinely check diagnostic results of SARS-CoV-2 and variants. Among blind tests of 136 clinical samples, the proposed system successfully diagnosed COVID-19 patients with a clinical sensitivity and specificity of 100% each. More importantly, the D614G (variant-common), T478K (delta-specific), and A67V (omicron-specific) mutations of the SARS-CoV-2 S gene were detected selectively, enabling the diagnosis of 70 SARS-CoV-2 delta or omicron variant patients. The DAMPR assay system is expected to be employed for on-site, rapid, accurate detection of SARS-CoV-2 and its variants gene and employed in the diagnosis of various infectious diseases.

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“…This SERS immunoassay detected SARS-CoV-2 S protein with a LOD of 0.046 ng mL −1 (0.60 pM). Cha et al [ 94 ] proposed a magnetically assisted immunosensor consisting of the reporter antibody, malachite green isothiocyanate conjugated hollow AuNPs, and antibody-conjugated magnetic beads for the SARS-CoV-2 determination. In this study, malachite green was utilized as a popular Raman dye that generates a distinct spectrum.…”

Section: Sers-based Sensing Methodsmentioning

confidence: 99%

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

2022

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The outbreak rate of human coronaviruses (CoVs) especially highly pathogenic CoVs is increasing alarmingly. Early detection of these viruses allows treatment interventions to be provided more quickly to people at higher risk, as well as helping to identify asymptomatic carriers and isolate them as quickly as possible, thus preventing the disease transmission chain. The current diagnostic methods such as RT-PCR are not ideal due to high cost, low accuracy, low speed, and probability of false results. Therefore, a reliable and accurate method for the detection of CoVs in biofluids can become a front-line tool in order to deal with the spread of these deadly viruses. Currently, the nanomaterial-based sensing devices for detection of human coronaviruses from laboratory diagnosis to point-of-care (PoC) diagnosis are progressing rapidly. Gold nanoparticles (AuNPs) have revolutionized the field of biosensors because of the outstanding optical and electrochemical properties. In this review paper, a detailed overview of AuNP-based biosensing strategies with the varied transducers (electrochemical, optical, etc.) and also different biomarkers (protein antigens and nucleic acids) was presented for the detection of human coronaviruses including SARS-CoV-2, SARS-CoV-1, and MERS-CoV and lowly pathogenic CoVs. The present review highlights the newest trends in the SARS-CoV-2 nanobiosensors from the beginning of the COVID-19 epidemic until 2022. We hope that the presented examples in this review paper convince readers that AuNPs are a suitable platform for the designing of biosensors. Graphical abstract

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Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cited by 42 publications

References 66 publications

Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader

Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader

Smartphone-Based SARS-CoV-2 and Variants Detection System using Colorimetric DNAzyme Reaction Triggered by Loop-Mediated Isothermal Amplification (LAMP) with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

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