Gut microbiota as prognosis markers for patients with HBV-related acute-on-chronic liver failure

Wang, Ke; Zhang, Zhao; Mo, Zhishuo; Yang, Xiaohua; Lin, Bingliang; Peng, Liang; Xu, Yang; Lei, Chunyan; Zhuang, Xiaodong; Lü, Ling; Chen, Tao; Gao, Zhiliang

doi:10.1080/19490976.2021.1921925

Cited by 18 publications

(9 citation statements)

References 46 publications

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“…Correspondingly, some beneficial bacterial taxa were decreased in SBP patients, such as Faecalibacterium prausnitzii , Methanobrevibacter smithii and Lactobacillus reuteri . The species F. prausnitzii has been demonstrated to have a lower abundance in patients with liver cirrhosis than in healthy people, and is associated with the regression of acute-on-chronic liver failure (ACLF) ( Wang et al., 2021 ; Chen et al., 2021 ). F. prausnitzii is the main butyrate-producing bacterium, and its short-chain fatty acid (SCFA) metabolites have an important anti-inflammatory effect on the intestine and liver ( Lee et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Alterations of gut microbiota in cirrhotic patients with spontaneous bacterial peritonitis: A distinctive diagnostic feature

Zhou

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundSpontaneous bacterial peritonitis (SBP) is a severe infection in cirrhotic patients that requires early diagnosis to improve the long-term outcome. Alterations in the gut microbiota have been shown to correlate with the development and progression of liver cirrhosis. However, the relationship between SBP and gut microbiota remains unknown.MethodsIn this study, we applied 16S rRNA pyrosequencing of feces to ascertain possible links between the gut microbiota and SBP. We recruited 30 SBP patients, 30 decompensated cirrhotic patients without SBP (NSBP) and 30 healthy controls. Metagenomic functional prediction of bacterial taxa was achieved using PICRUSt. ResultsThe composition of the gut microbiota in the SBP patients differed remarkably from that in the NSBP patients and healthy individuals. The microbial richness was significantly decreased, while the diversity was increased in the SBP patients. Thirty-four bacterial taxa containing 15 species, mainly pathogens such as Klebsiella pneumoniae, Serratia marcescens and Prevotella oris, were dominant in the SBP group, while 42 bacterial taxa containing 16 species, especially beneficial species such as Faecalibacterium prausnitzii, Methanobrevibacter smithii and Lactobacillus reuteri, were enriched in the NSBP group. Notably, we found that 18 gene functions of gut microbiota were different between SBP patients and NSBP patients, which were associated with energy metabolism and functional substance metabolism. Five optimal microbial markers were determined using a random forest model, and the combination of Lactobacillus reuteri, Rothia mucilaginosa, Serratia marcescens, Ruminococcus callidus and Neisseria mucosa achieved an area under the curve (AUC) value of 0.8383 to distinguish SBP from decompensated cirrhosis.ConclusionsWe described the obvious dysbiosis of gut microbiota in SBP patients and demonstrated the potential of microbial markers as noninvasive diagnostic tools for SBP at an early stage.

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Section: Discussionmentioning

confidence: 99%

Alterations of gut microbiota in cirrhotic patients with spontaneous bacterial peritonitis: A distinctive diagnostic feature

Zhou

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Another species of our great interest is Alistipes_senegalensis. Studies have confirmed that it is negatively correlated with the progression of chronic liver failure ( Wang et al, 2021 ) and it was also significantly reduced in the gut microorganisms of Crohn’s disease patients ( Hu et al, 2021 ), which may suggest that Alistipes_senegalensis plays a protective role in chronic kidney disease. These results suggested that finasteride may play a renal protective role by positively modulating beneficial intestinal microbiome.…”

Section: Discussionmentioning

confidence: 95%

Finasteride Alleviates High Fat Associated Protein-Overload Nephropathy by Inhibiting Trimethylamine N-Oxide Synthesis and Regulating Gut Microbiota

et al. 2022

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Unhealthy diet especially high-fat diet (HFD) is the major cause of hyperlipidemia leading to deterioration of chronic kidney diseases (CKD) in patients. Trimethylamine N-oxide (TMAO) is a gut-derived uremic toxin. Our previous clinical study demonstrated that the elevation of TMAO was positively correlated with CKD progression. Finasteride, a competitive and specific inhibitor of type II 5a-reductase, has been reported recently to be able to downregulate plasma TMAO level thus preventing the onset of atherosclerosis by our research group. In this study, we established a protein-overload nephropathy CKD mouse model by bovine serum albumin (BSA) injection to investigate whether hyperlipidemia could accelerate CKD progression and the underlying mechanisms. Finasteride was administrated to explore its potential therapeutic effects. The results of biochemical analyses and pathological examination showed that HFD-induced hyperlipidemia led to aggravated protein-overload nephropathy in mice along with an elevated level of circulating TMAO, which can be alleviated by finasteride treatment possibly through inhibition of Fmo3 in liver. The 16 S rRNA sequencing results indicated that HFD feeding altered the composition and distribution of gut microbiota in CKD mice contributing to the enhanced level of TMAO precursor TMA, while finasteride could exert beneficial effects via promoting the abundance of Alistipes_senegalensis and Akkermansia_muciniphila. Immunofluorescence staining (IF) and qRT-PCR results demonstrated the disruption of intestinal barrier by decreased expression of tight junction proteins including Claudin-1 and Zo-1 in HFD-fed CKD mice, which can be rescued by finasteride treatment. Cytokine arrays and redox status analyses revealed an upregulated inflammatory level and oxidative stress after HFD feeding in CKO mice, and finasteride-treatment could alleviate these lesions. To summarize, our study suggested that finasteride could alleviate HFD-associated deterioration of protein-overload nephropathy in mice by inhibition of TMAO synthesis and regulation of gut microbiota.

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“…From healthy to CHB, Bilophila decreased significantly, whereas an unclassified Lachnospiraceae taxa was enriched. Bilophila was found as negatively associated with ALT and AST, suggesting its relevance to attenuated liver inflammation (Wang et al, 2021 ). From CHB to LC, Oscillibacter, Oscillospiraceae_UCG-005 , and Prevotella were depleted.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease

Yang

et al. 2022

Front. Microbiol.

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The gut microbiome is associated with hepatitis B virus (HBV)-induced liver disease, which progresses from chronic hepatitis B, to liver cirrhosis, and eventually to hepatocellular carcinoma. Studies have analyzed the gut microbiome at each stage of HBV-induced liver diseases, but a consensus has not been reached on the microbial signatures across these stages. Here, we conducted by a systematic meta-analysis of 486 fecal samples from publicly available 16S rRNA gene datasets across all disease stages, and validated the results by a gut microbiome characterization on an independent cohort of 15 controls, 23 chronic hepatitis B, 20 liver cirrhosis, and 22 hepatocellular carcinoma patients. The integrative analyses revealed 13 genera consistently altered at each of the disease stages both in public and validation datasets, suggesting highly robust microbiome signatures. Specifically, Colidextribacter and Monoglobus were enriched in healthy controls. An unclassified Lachnospiraceae genus was specifically elevated in chronic hepatitis B, whereas Bilophia was depleted. Prevotella and Oscillibacter were depleted in liver cirrhosis. And Coprococcus and Faecalibacterium were depleted in hepatocellular carcinoma. Classifiers established using these 13 genera showed diagnostic power across all disease stages in a cross-validation between public and validation datasets (AUC = 0.65–0.832). The identified microbial taxonomy serves as non-invasive biomarkers for monitoring the progression of HBV-induced liver disease, and may contribute to microbiome-based therapies.

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Gut microbiota as prognosis markers for patients with HBV-related acute-on-chronic liver failure

Abstract: Gao (2021) Gut microbiota as prognosis markers for patients with HBV-related acute-on-chronic liver failure,

Cited by 18 publications

References 46 publications

Alterations of gut microbiota in cirrhotic patients with spontaneous bacterial peritonitis: A distinctive diagnostic feature

Alterations of gut microbiota in cirrhotic patients with spontaneous bacterial peritonitis: A distinctive diagnostic feature

Finasteride Alleviates High Fat Associated Protein-Overload Nephropathy by Inhibiting Trimethylamine N-Oxide Synthesis and Regulating Gut Microbiota

Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease

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