Capnocytophaga canimorsus meningitis diagnosed using next- generation sequencing of microbial cell-free DNA

Asif, Abuzar A; Roy, Moni; Tellier, Benjamin R.; Ahmad, Sharjeel

doi:10.1016/j.idcr.2021.e01126

Cited by 5 publications

(3 citation statements)

References 20 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NGS cfDNA can be applied to other infections, such as C. canimorsus meningitis. Moreover, antibiotic exposure does not affect this diagnostic method [41].…”

Section: Neuroinfectious Diseasesmentioning

confidence: 99%

“…Neuroinfectious diseases Bacterial meningitis Mycobacterium tuberculosis cfDNA (IS6110 sequence) CSF -Its amplification is more sensitive than some traditional diagnostic protocols [41] Capnocytophaga canimorsus meningitis cfDNA Blood -Diagnostic method is not affected by antibiotic exposure [41] Neurocysticercosis (infection by Taenia solium)…”

Section: Biomarker Source Main Findingsmentioning

confidence: 99%

See 1 more Smart Citation

Liquid Biopsy in Neurological Diseases

Comabella

Miras

Pappolla

et al. 2023

Cells

View full text Add to dashboard Cite

The most recent and non-invasive approach for studying early-stage biomarkers is liquid biopsy. This implies the extraction and analysis of non-solid biological tissues (serum, plasma, saliva, urine, and cerebrospinal fluid) without undergoing invasive procedures to determine disease prognosis. Liquid biopsy can be used for the screening of several components, such as extracellular vesicles, microRNAs, cell-free DNA, cell-free mitochondrial and nuclear DNA, circulating tumour cells, circulating tumour DNA, transfer RNA, and circular DNA or RNA derived from body fluids. Its application includes early disease diagnosis, the surveillance of disease activity, and treatment response monitoring, with growing evidence for validating this methodology in cancer, liver disease, and central nervous system (CNS) disorders. This review will provide an overview of mentioned liquid biopsy components, which could serve as valuable biomarkers for the evaluation of complex neurological conditions, including Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, epilepsy, stroke, traumatic brain injury, CNS tumours, and neuroinfectious diseases. Furthermore, this review highlights the future directions and potential limitations associated with liquid biopsy.

show abstract

“…NGS cfDNA can be applied to other infections, such as C. canimorsus meningitis. Moreover, antibiotic exposure does not affect this diagnostic method [41].…”

Section: Neuroinfectious Diseasesmentioning

confidence: 99%

Section: Biomarker Source Main Findingsmentioning

confidence: 99%

Liquid Biopsy in Neurological Diseases

Comabella

Miras

Pappolla

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

“…Circulating microbial cfDNA can be used to diagnose CNS infection when cultures and PCR testing for common organisms fail, as is demonstrated by a recent case study featuring a patient with a rare form of bacterial meningitis. 176 Importantly, the diagnostic method was not impacted by antibiotic exposure, an essential characteristic of diagnostic tests for severe infections requiring immediate treatment. While the methodology used in this instance—applying NGS to cfDNA isolates from blood samples—is too expensive and time-consuming to be translatable to most clinical settings, it demonstrates the potential utility of identifying microbe-specific cfDNA signatures.…”

Section: Recent Developments and Applications Of Cfdna-based Liquid B...mentioning

confidence: 99%

Cell-free DNA-based liquid biopsies in neurology

Gaitsch

Franklin²,

Reich

2022

Brain

View full text Add to dashboard Cite

This article reviews recent developments in the application of cell-free DNA-based liquid biopsies to neurological diseases. Over the past few decades, an explosion of interest in the use of accessible biofluids to identify and track molecular disease has revolutionized the fields of oncology, prenatal medicine, and others. More recently, technological advances in signal detection have allowed for informative analysis of biofluids that are typically sparse in cells and other circulating components, such as cerebrospinal fluid. In parallel, advancements in epigenetic profiling have allowed for novel applications of liquid biopsies to diseases without characteristic mutational profiles, including many degenerative, autoimmune, inflammatory, ischaemic, and infectious disorders. These events have paved the way for a wide array of neurological conditions to benefit from enhanced diagnostic, prognostic, and treatment abilities through the use of liquid biomarkers: a “liquid biopsy” approach. This review includes an overview of types of liquid biopsy targets with a focus on circulating cell-free DNA, methods used to identify and probe potential liquid biomarkers, and recent applications of such biomarkers to a variety of complex neurological conditions including CNS tumours, stroke, traumatic brain injury, Alzheimer’s disease, epilepsy, multiple sclerosis, and neuroinfectious disease. Finally, the challenges of translating liquid biopsies to use in clinical neurology settings—and the opportunities for improvement in disease management that such translation may provide—are discussed.

show abstract

Underestimating a Dog Bite

Amadori¹,

Fabiani

Lenzi³

et al. 2023

Infect Dis Clin Pract

View full text Add to dashboard Cite

Dog bites may result in serious bacterial infections because of various microorganisms, including gram-negative rod Capnocytophaga canimorsus, whose disease can be complicated by acute development of sepsis and/or meningitis. In this review, we report a case of C. canimorsus meningitis in a patient with major risk factors and subsequently analyze 48 other cases described in literature. Approximately a little less than 50% of reported cases had a major predisposing condition such as splenectomy, alcoholism, or immunosuppressive treatment. In almost all of the cases, recent animal exposure was reported. Sensorineural hearing loss, which has been described as a permanent complication of C. canimorsus meningitis, was present in a minority of cases (18%), and a possible association with alcoholism was observed. In conclusion, C. canimorsus should be considered as a possible cause of sporadic meningitis in adults, particularly if presenting with a subacute course or deafness and in concomitance of major predisposing conditions, such as alcoholism.

show abstract

Capnocytophaga canimorsus meningitis diagnosed using next- generation sequencing of microbial cell-free DNA

Cited by 5 publications

References 20 publications

Liquid Biopsy in Neurological Diseases

Liquid Biopsy in Neurological Diseases

Cell-free DNA-based liquid biopsies in neurology

Underestimating a Dog Bite

Contact Info

Product

Resources

About