Determination of the LD<sub>50</sub> with the chick embryo chorioallantoic membrane (CAM) assay as a promising alternative in nanotoxicological evaluation

Buhr, Christoph Raphael; Eckrich, Jonas; Kluenker, Martin; Bruns, Kai; Wiesmann, Nadine; Tremel, Wolfgang; Brieger, Jürgen

doi:10.1080/17435390.2021.1916635

Cited by 6 publications

(6 citation statements)

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“…Since liver and kidney are the organs responsible for the excretion these are the typical sites where nanoparticles are deposited. 78,79 Taken together, polypept(o)ides and ruthenium(II) complexes were successfully combined in photocleavable core cross-linked polymeric micelles. The cross-linking by the ruthenium(II) complexes provided colloidal stability, whereby the choice of the nitrile linker for complex conjugation showed a critical influence on the particle morphology.…”

Section: Discussionmentioning

confidence: 99%

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Bauer

Eckrich²,

Wiesmann³

et al. 2021

J. Mater. Chem. B

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Section: Discussionmentioning

confidence: 99%

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Bauer

Eckrich²,

Wiesmann³

et al. 2021

J. Mater. Chem. B

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“…95 The CAM has been applied to various types of NPs. 96,66,97 Dose-dependent cytotoxic effects on endothelial cells, and blood vessels formation inhibition were shown by silver nanoparticles using CAM model. 97 This CAM assay can be possibly developed for toxicity evaluation of NPs or EDCs if the negative biological outcome is determined, such as increased tumor burden, or abnormal development of blood vessels in the chicken embryos.…”

Section: Micronucleus (Mn) Test and Cometmentioning

confidence: 99%

“…96,66,97 Dose-dependent cytotoxic effects on endothelial cells, and blood vessels formation inhibition were shown by silver nanoparticles using CAM model. 97 This CAM assay can be possibly developed for toxicity evaluation of NPs or EDCs if the negative biological outcome is determined, such as increased tumor burden, or abnormal development of blood vessels in the chicken embryos. The CAM assay as an in vivo model was also used to study the effects of anticancer drug (doxorubicin)-loaded (biodegradable PMO) NP in ovarian cancer, with tumor development after injection of tumor cells but decreased tumor burden later after the injection of the NPs anticancer drug.…”

Section: Micronucleus (Mn) Test and Cometmentioning

confidence: 99%

“…Ex ovo provides large CAM area, easy visibility and access to CAM vasculature, easy grafting and evaluation of several samples in one embryo but can have low embryo survival rate and may not reflect physiological condition as in in ovo . The CAM has been applied to various types of NPs. ,, Dose-dependent cytotoxic effects on endothelial cells, and blood vessels formation inhibition were shown by silver nanoparticles using CAM model . This CAM assay can be possibly developed for toxicity evaluation of NPs or EDCs if the negative biological outcome is determined, such as increased tumor burden, or abnormal development of blood vessels in the chicken embryos.…”

Section: Major Techniques Used In the Chicken Embryo Models For Toxic...mentioning

confidence: 99%

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Use of Chicken Embryo Model in Toxicity Studies of Endocrine-Disrupting Chemicals and Nanoparticles

Ghimire

Zhang

et al. 2022

Chem. Res. Toxicol.

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Lab animals such as mice and rats are widely used in toxicity research of food additive and pharmaceutics, despite the wellrecognized research limitation such as the inability to simulate human neurological diseases, faster absorption of chemicals, big variations among species, and high cost when using a large number of animals. The Society of Toxicology's guidance now focuses on minimizing discomfort and distress of lab animals, finding alternative ways to reduce animal number, replacing animals with in vitro models, and complying to the animal welfare policies. The chicken embryonic model can be a better alternative to mice and rats because of its abundant availability and cost-effectiveness. It can be studied in both laboratory and natural environment, with easy manipulation in ovo or in vivo. The objective of this review paper is to evaluate the use of chicken embryonic model in toxicity evaluation for endocrine-disrupting chemicals (EDCs) and nanoparticles (NPs) by different end points to determine more comprehensive toxic responses. The end points include chicken embryonic mortality and hatchability, developmental malformation analysis, hormonal imbalance, physiological changes in endocrine organs, and antiangiogenesis. Major research methodologies using chicken embryos are also summarized to demonstrate their versatile practice and valuable application in modern toxicity evaluation of EDCs and NPs.

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“…Previous studies already proved that the chicken chorioallantoic membrane assay (CAM assay) is well suited for assessing the biocompatibility of nanomaterials [32], their fate within the organism, and hemocompatibility, as well as potential toxic effects [33]. Furthermore, the CAM is an excellent platform with which to study angiogenesis [34] and the angiogenetic activity of biomaterials [35,36].…”

Section: Chicken Chorioallantoic Membrane Assay (Cam-assay)mentioning

confidence: 99%

Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

et al. 2021

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Due to the demographic change, medicine faces a growing demand for tissue engineering solutions and implants. Often, satisfying tissue regeneration is difficult to achieve especially when co-morbidities hamper the healing process. As a novel strategy, we propose the incorporation of zinc oxide nanoparticles (ZnO NPs) into biomaterials to improve tissue regeneration. Due to their wide range of biocompatibility and their antibacterial properties, ZnO NPs are already discussed for different medical applications. As there are versatile possibilities of modifying their form, size, and function, they are becoming increasingly attractive for tissue engineering. In our study, in addition to antibacterial effects of ZnO NPs, we show for the first time that ZnO NPs can foster the metabolic activity of fibroblasts as well as endothelial cells, both cell types being crucial for successful implant integration. With the gelatin sponge method performed on the chicken embryo’s chorioallantoic membrane (CAM), we furthermore confirmed the high biocompatibility of ZnO NPs. In summary, we found ZnO NPs to have very favorable properties for the modification of biomaterials. Here, incorporation of ZnO NPs could help to guide the tissue reaction and promote complication-free healing.

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Determination of the LD₅₀ with the chick embryo chorioallantoic membrane (CAM) assay as a promising alternative in nanotoxicological evaluation

Cited by 6 publications

References 62 publications

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Use of Chicken Embryo Model in Toxicity Studies of Endocrine-Disrupting Chemicals and Nanoparticles

Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

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Determination of the LD50 with the chick embryo chorioallantoic membrane (CAM) assay as a promising alternative in nanotoxicological evaluation

Cited by 6 publications

References 62 publications

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Photocleavable core cross-linked polymeric micelles of polypept(o)ides and ruthenium(ii) complexes

Use of Chicken Embryo Model in Toxicity Studies of Endocrine-Disrupting Chemicals and Nanoparticles

Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

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Determination of the LD₅₀ with the chick embryo chorioallantoic membrane (CAM) assay as a promising alternative in nanotoxicological evaluation