Acute exposure to microcystin-LR induces hepatopancreas toxicity in the Chinese mitten crab (Eriocheir sinensis)

Cheng, Chunmei; Yu, Xiawei; Zhang, Caiyan; Liu, Jia-Dai; Ye, Ming-Wen; Zhang, Dingdong; Liu, Wenbin

doi:10.1007/s00204-021-03061-9

Cited by 13 publications

(3 citation statements)

References 53 publications

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“…Antioxidant systems were efficient defenders of organisms against the negative impacts of toxicants. In E. sinensis, short-term acute toxicity of MC-LR (within 48 h) often caused enhanced antioxidant ability [5,24]. Contrast to that, we found prolonged exposure (7 d) to Microcystis spp.…”

Section: Impact Of Cyanobacterial Bloom On E Sinensis Culture Pondcontrasting

confidence: 88%

“…In E. sinensis culture ponds, cyanobacterial blooms are commonly formed by the following genera, e.g., Microcystis, Cyanobium, Calothrix, Sphaerospermopsis and Cylindrospermopsis [1]. It is well documented that cyanobacterial blooms and their secondary metabolites, cyanobacterial toxins (such as microcystins, anatoxin-a, retinoic acid, microviridins, anabaenopeptins, aeruginosins, microginins, piricyclamides, and cyanopeptolins) can cause a series of problems in aquaculture, such as compromising water quality, altering microbial community assembly, toxic to aquatic animals, and even threatening to human health by bioaccumulation through food chains [5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Impact of Effective Microorganisms and Chlorella vulgaris on Eriocheir sinensis and Water Microbiota in Ponds Experiencing Cyanobacterial Blooms

et al. 2023

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Cyanobacterial blooms threaten the quality and safety of the Chinese mitten crab Eriocheir sinensis. A combination of microalgae and probiotics seems a promising way to prevent and control cyanobacterial blooms in aquaculture ponds. In E. sinensis cultivation, however, a related strategy is still lacking. To assess the potential combined effects of effective microorganisms (EM) and Chlorella vulgaris on regulating cyanobacterial blooms, in this study, we detected the alterations in the physiology of E. sinensis, as well as water quality and microbial compositions of E. sinensis culture ponds with cyanobacterial blooms. As a result, supplementary EM and C. vulgaris had no adverse effects on the growth or digestive or antioxidant ability of E. sinensis but improved the water quality of the pond by reducing total ammonia nitrogen and total nitrogen levels. We found an increase in bacterial diversity and evenness, while a decrease in the diversity of fungal and phytoplankton communities was related to supplementary EM and C. vulgaris. Interestingly, EM coupling C. vulgaris promoted the restoration of the bacterial and fungal community composition in cyanobacterial blooms ponds, particularly the increase of Mychonastes abundance and the decrease of Cyclotella. This study laid the foundation for the prevention and control of potential risks in aquaculture.

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Section: Impact Of Cyanobacterial Bloom On E Sinensis Culture Pondcontrasting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Impact of Effective Microorganisms and Chlorella vulgaris on Eriocheir sinensis and Water Microbiota in Ponds Experiencing Cyanobacterial Blooms

et al. 2023

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“…In the mitochondrial pathways, MCs can cause inhibition of protein phosphatases, change of reactive oxygen species content, antioxidant dysfunctions ( Sebastián et al, 2015 ; Min et al, 2018 ; Zhang et al, 2019 ), enhancement of apoptosis-related enzyme activities, cell apoptosis ( Chen et al, 2021 ; Sun et al, 2021 ), enhancement of apoptosis-related enzyme activities, cell apoptosis ( Chen et al, 2021 ; Chi et al, 2021 ; ), and collapse of microfilaments, microtubules and intermediate filaments of the cytoskeleton ( Zhou et al, 2015 ). MCs-induced accumulations of unfolded or misfolded proteins in the endoplasmic reticulum cavity leads to apoptosis through transcriptional induction of CHOP ( Oyadomari and Mori, 2004 ) and/or caspase-12 dependent pathway ( Szegezdi et al, 2003 ) in the endoplasmic reticulum pathways.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of mRNA and microRNA expression profiles in hepatopancreas of Litopenaeus vannamei under acute exposure to MC-LR

Zhang

Huang²,

Yang³

et al. 2023

Front. Genet.

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Intensive shrimp farming is often threatened by microcystins Hepatopancreas is the primary target organ of MCs in shrimp. To investigate the response of hepatopancreas to acute MC-LR exposure, the expression profiles of RNA-seq and miRNA-seq in the hepatopancreas of L. vannamei were determined, and data integration analysis was performed at 72 h after MC-LR injection. The expression of 5 DEGs and three DEMs were detected by Quantitative PCR (qPCR). The results showed that the cumulative mortality rate of shrimp in MC-LR treatment group was 41.1%. A total of 1229 differentially expressed genes (844 up- and 385 down-regulated) and 86 differentially expressed miRNAs (40 up- and 46 down-regulated) were identified after MC-LR exposure. Functional analysis indicated that DEGs is mainly involved in the oxidative activity process in molecular functional categories, and proteasome was the most enriched KEGG pathway for mRNAs profile. According to the functional annotation of target genes of DEMs, protein binding was the most important term in the GO category, and protein processing in endoplasmic reticulum (ER) was the most enriched KEGG pathway. The regulatory network of miRNAs and DEGs involved in the pathway related to protein degradation in endoplasmic reticulum was constructed, and miR-181-5p regulated many genes in this pathway. The results of qPCR showed that there were significant differences in the expression of five DEGs and three DEMs, which might play an important role in the toxicity and hepatopancreas detoxification of MC-LR in shrimp. The results revealed that MC-LR exposure affected the degradation pathway of misfolded protein in ER of L. vannamei hepatopancreas, and miR-181-5p might play an important role in the effect of MC-LR on the degradation pathway of misfolded protein.

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Histological observations and gene expression of the digestive system of the Chinses mitten crab (Eriocheir sinensis)

Zhang,

Jiang,

Liu

et al. 2023

Aquaculture and Fisheries

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Acute exposure to microcystin-LR induces hepatopancreas toxicity in the Chinese mitten crab (Eriocheir sinensis)

Cited by 13 publications

References 53 publications

Impact of Effective Microorganisms and Chlorella vulgaris on Eriocheir sinensis and Water Microbiota in Ponds Experiencing Cyanobacterial Blooms

Impact of Effective Microorganisms and Chlorella vulgaris on Eriocheir sinensis and Water Microbiota in Ponds Experiencing Cyanobacterial Blooms

Integrated analysis of mRNA and microRNA expression profiles in hepatopancreas of Litopenaeus vannamei under acute exposure to MC-LR

Histological observations and gene expression of the digestive system of the Chinses mitten crab (Eriocheir sinensis)

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