Sensitive recognition of ractopamine using GQDs‐DPA as organic fluorescent probe

Abstract

A novel spectrofluorimetric sensing platform was designed for Ractopamine measurement in aqueous and plasma samples. D-penicillamine functionalized graphene quantum dots (DPA-GQDs) was utilized as a fluorescence probe, which was synthesized through the pyrolysis of citric acid in the presence of DPA. This one-pot downtop strategy causes to high-yield controllable synthesis method. The reaction time and probe concentration were optimized. Then, the fluorescence intensity of aqueous samples containing different Ractopamine concentrations and 500 ppm DPA-GQDs were measured at 25 C with an excitation wavelength of 274 nm. The sensing platform was also applied to detect Ractopamine in untreated plasma samples. The fluorescence spectroscopy technique responses indicated a linear relationship between the peak fluorescence intensity and ractopamine concentration in the range of 0.25-15 ppm with low limit of quantification of 0.25 ppm was for aqueous and plasma samples, respectively.

Supramolecular recognition enhanced electrochemical sensing: β-cyclodextrin and Pd nanoparticle co-decorated 3D reduced graphene oxide nanocomposite-modified glassy carbon electrode for the quantification of ractopamine

“…Compared with other electrochemical RAC detection that use DPV methods in the literature (Table S1 †), 3D-rGO/Pd/b-CD/GCE exhibites a wider detection range and lower detection limit. 11,12,20,[36][37][38][39][40][41][42][43][44][45][46] It is noteworthy that the proposed method is also superior to the other reported RAC detection methods listed in Table S1. † This is because, despite the ultra-low sensitivity or wide detection range of some methods, they oen utilize expensive instruments and complicated procedures for the determination of RAC.…”

“…At present, various analytical techniques, including enzyme-linked immunoassay, 7 immunochromatography, 8 chromatography, 9 electrophoresis, 10 fluorescence, 11 and electrochemiluminescence, 12 have been reported for the detection of RAC. Notwithstanding their efficacy, these strategies are beset by time consumption and the need for costly instrumentation, skilled technicians, and pretreatment processes.…”

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

“…Zhao et al created a simple synthetic method for GQDs by carbonizing l -glutamic acid with a heating mantle device . In a recent work, a fluorescent probe, d -penicillamine (DPA) functionalized GQDs were used, which were made by pyrolyzing citric acid in the presence of DPA for ractopamine quantification in aqueous and plasma samples . The pyrolysis method is also used with hydrothermal for the preparation of N, S-GQDs@Au-polyaniline amperometric immunosensor to detect carcinoembryonic antigen …”