A novel spectrofluorimetric sensing platform was designed for Ractopamine measurement in aqueous and plasma samples. D-penicillamine functionalized graphene quantum dots (DPA-GQDs) was utilized as a fluorescence probe, which was synthesized through the pyrolysis of citric acid in the presence of DPA. This one-pot downtop strategy causes to high-yield controllable synthesis method. The reaction time and probe concentration were optimized. Then, the fluorescence intensity of aqueous samples containing different Ractopamine concentrations and 500 ppm DPA-GQDs were measured at 25 C with an excitation wavelength of 274 nm. The sensing platform was also applied to detect Ractopamine in untreated plasma samples. The fluorescence spectroscopy technique responses indicated a linear relationship between the peak fluorescence intensity and ractopamine concentration in the range of 0.25-15 ppm with low limit of quantification of 0.25 ppm was for aqueous and plasma samples, respectively.
An innovative electrochemical biosensor for the sensitive and specific detection of l-proline (l-Pro) in human plasma samples was prepared by the encapsulation of proline dehydrogenase on amine functionalized dendritic fibrous nano-silica KCC-1.
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