Xiao-Yin-Fang Therapy Alleviates Psoriasis-like Skin Inflammation Through Suppressing γδT17 Cell Polarization

Abstract

Psoriasis is an immune-mediated chronic inflammatory skin disease primarily mediated by the activation of interleukin (IL)-17-producing T cells. Traditional Chinese Medicine (TCM) represents one of the most effective complementary and alternative medicine (CAM) agents for psoriasis, which provides treasured sources for the development of anti-psoriasis medications. Xiao-Yin-Fang (XYF) is an empirically developed TCM formula that has been used to treat psoriasis patients in Shanghai Changhai Hospital for over three decades. Imiquimod (IMQ)-induced psoriasis-like dermatitis mouse model was utilized to investigate the therapeutic effects of XYF by the assessment of disease severity and skin thickness. Flow cytometric assay was performed to explore the influence of XYF on skin-related immunocytes, primarily T cells. And, RNA sequencing analysis was employed to determine the alternation in gene expression upon XYF therapy. We discovered that XYF alleviated psoriasis-like skin inflammation mainly through suppressing dermal and draining lymph-node IL-17-producing γδT (γδT17) cell polarization. Moreover, XYF therapy ameliorated the relapse of psoriasis-like dermatitis and prohibited dermal γδT cell reactivation. Transcriptional analysis suggested that XYF might regulate various inflammatory signaling pathways and metabolic processes. In conclusion, our results clarified the therapeutic efficacy and inner mechanism of XYF therapy in psoriasis, which might promote its clinical application in psoriasis patients and facilitate the development of novel anti-psoriasis drugs based on the bioactive components of XYF.

Potential mechanism of oral baicalin treating psoriasis via suppressing Wnt signaling pathway and inhibiting Th17/IL‐17 axis by activating PPARγ

“…Except for the untreated control group (CON), mice in other groups were administered topically at a daily dose of 62.5 mg of a cream preparation containing 5% IMQ on the shaved back (2 × 3 cm) of each mouse to induce the psoriasis model. Mice in the positive control group were administered intragastrically with 40 mg/kg/d TWP tablets (TWP, a patented Chinese drug widely used for the clinical treatment of psoriasis in China) (Wu et al, 2015; Zhang et al, 2021; Zhao et al, 2016). The oral dose of HQG was determined according to a previous clinical observation study (Chin J Dermatovenereol.…”

“…Scutellaria baicalensis is a commonly used herbal medicine in traditional Chinese medicine, which has been found in the prescriptions for the treatment of PSO such as Xiao‐Yin‐Fang (Zhang et al, 2021). Chemical studies have shown that flavonoids and their glycosides are the main effective substance of Scutellaria baicalensis .…”

“…Scutellaria baicalensis is a commonly used herbal medicine in traditional Chinese medicine, which has been found in the prescriptions for the treatment of PSO such as Xiao-Yin-Fang (Zhang et al, 2021).…”

Emodin Combined with Multiple-Low-Frequency, Low-Intensity Ultrasound To Relieve Osteomyelitis through Sonoantimicrobial Chemotherapy

“…Because human cells have no cell wall structure, this antibacterial therapy does less damage to human cells. Because EM has broad-spectrum effects, including anti-inflammatory, antioxidant, and antibacterial effects ( 42 ), we used a confocal probe and flow cytometry to detect whether M-LFLIU could enhance the antibacterial effect of EM-stimulated ROS. In the process of the body’s fight against bacteria, proper ROS promotes the body’s immunity and repair.…”

The anti‐inflammatory activity of flavonoids and alkaloids from Sophora flavescens alleviates psoriasiform lesions: Prenylation and methoxylation beneficially enhance bioactivity and skin targeting

“…In modern pharmacology, S. flavescens has been found to be beneficial for treating inflammatory disorders such as eczema, colpitis, ulcerative colitis, and psoriasis (Ma et al, 2018). Some complex formulas containing S. flavescens have been proven to alleviate inflammatory skin diseases, including contact dermatitis, alopecia, and psoriasis (H. W. Kim et al, 2012; Takahashi et al, 2016; X. Zhang et al, 2021). Phytochemical assays identified that S. flavescens contains abundant flavonoids, quinolizidine alkaloids, and triterpenoids (Dong et al, 2021).…”