Emodin Combined with Multiple-Low-Frequency, Low-Intensity Ultrasound To Relieve Osteomyelitis through Sonoantimicrobial Chemotherapy

Lu, Feng; Wu, Xinhui; Hu, Huiqun; He, Zixuan; Sun, Jiacheng; Zhang, Jiapeng; Song, Xiaoting; Jin, Xiangang; Chen, Guofu

doi:10.1128/spectrum.00544-22

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Our research team found that EMO can exert anti-inflammatory effects through modulation of the Toll-like receptor 4/nuclear factor kappa-B (TLR4/NF-κB) signaling pathway for the treatment of ulcerative colitis [ 15 ]. In addition, EMO is also widely used in various inflammatory diseases, such as osteomyelitis, pneumonia and nephritis [ 16 , 17 ]. Building on these findings, our previous research delved into the promising anti-inflammatory effects of EMO in the context of CNP.…”

Section: Introductionmentioning

confidence: 99%

Thermosensitive hydrogel with emodin-loaded triple-targeted nanoparticles for a rectal drug delivery system in the treatment of chronic non-bacterial prostatitis

Ye,

Zhong,

Luo

et al. 2024

J Nanobiotechnol

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Background The complex etiology and pathogenesis underlying Chronic Non-Bacterial Prostatitis (CNP), coupled with the existence of a Blood Prostate Barrier (BPB), contribute to a lack of specificity and poor penetration of most drugs. Emodin (EMO), a potential natural compound for CNP treatment, exhibits commendable anti-inflammatory, anti-oxidant, and anti-fibrosis properties but suffers from the same problems as other drugs. Methods By exploiting the recognition properties of lactoferrin (LF) receptors that target intestinal epithelial cells (NCM-460) and prostate epithelial cells (RWPE-1), a pathway is established for the transrectal absorption of EMO to effectively reach the prostate. Additionally, hyaluronic acid (HA) is employed, recognizing CD44 receptors which target macrophages within the inflamed prostate. This interaction facilitates the intraprostatic delivery of EMO, leading to its pronounced anti-inflammatory effects. A thermosensitive hydrogel (CS-Gel) prepared from chitosan (CS) and β-glycerophosphate disodium salt (β-GP) was used for rectal drug delivery with strong adhesion to achieve effective drug retention and sustained slow release. Thus, we developed a triple-targeted nanoparticle (NPs)/thermosensitive hydrogel (Gel) rectal drug delivery system. In this process, LF, with its positive charge, was utilized to load EMO through dialysis, producing LF@EMO-NPs. Subsequently, HA was employed to encapsulate EMO-loaded LF nanoparticles via electrostatic adsorption, yielding HA/LF@EMO-NPs. Finally, HA/LF@EMO-NPs lyophilized powder was added to CS-Gel (HA/LF@EMO-NPs Gel). Results Cellular assays indicated that NCM-460 and RWPE-1 cells showed high uptake of both LF@EMO-NPs and HA/LF@EMO-NPs, while Raw 264.7 cells exhibited substantial uptake of HA/LF@EMO-NPs. For LPS-induced Raw 264.7 cells, HA/LF@EMO-NPs can reduce the inflammatory responses by modulating TLR4/NF-κB signaling pathways. Tissue imaging corroborated the capacity of HA/LF-modified formulations to breach the BPB, accumulating within the gland's lumen. Animal experiments showed that rectal administration of HA/LF@EMO-NPs Gel significantly reduced inflammatory cytokine expression, oxidative stress levels and fibrosis in the CNP rats, in addition to exerting anti-inflammatory effects by inhibiting the NF-κB signaling pathway without obvious toxicity. Conclusion This triple-targeted NPs/Gel rectal delivery system with slow-release anti-inflammatory, anti-oxidant, and anti-fibrosis properties shows great potential for the effective treatment of CNP. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Thermosensitive hydrogel with emodin-loaded triple-targeted nanoparticles for a rectal drug delivery system in the treatment of chronic non-bacterial prostatitis

Ye,

Zhong,

Luo

et al. 2024

J Nanobiotechnol

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“…In the anti-infection field, physical LFU can not only enhance the anti-infection ability of antibiotics but also reduce the risk of bacterial antibiotic resistance ( 19 ). Other researchers and we have found that LFU can improve the antibacterial effect of various antibiotics on planktonic and biofilm bacteria in vitro and in vivo , such as MRSA, Pseudomonas aeruginosa , and Klebsiella pneumoniae ( 20 – 22 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of low-frequency ultrasound combined with anti-MRSA agents on the mouse model of pulmonary infection

Yan,

Yao,

Liu

et al. 2024

Microbiol Spectr

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Treating pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA) with antibiotics alone poses significant challenges. In this in vivo study, we present compelling evidence supporting the efficacy of low-frequency ultrasound (LFU) as a promising approach to overcome these obstacles. Our findings demonstrated that LFU enhanced the effectiveness of vancomycin, linezolid, and contezolid in an MRSA pneumonia model. The combination of LFU with anti-MRSA agents markedly improved the survival rate of mice, accelerated the clearance of pulmonary bacteria, reduced inflammatory injury, inhibited the production of MRSA endotoxin, and enhanced the distribution of antibiotics in lung tissue. The application of LFU in the treatment of pulmonary infections held substantial significance. We believe that readers of your journal will find this topic of considerable interest.

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Characteristics of the Results of Electrical Exposure When Inducing Osteomyelitis in Rats by Different Types of Patients

Ovchinnikov,

Kubrak,

Diuriagina

et al. 2024

jour

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The aim of the research. To study the features of the course of osteomyelitis in rats during the induction of an infectious and inflammatory process by various pathogens under conditions of early and delayed local exposure to direct electric current. Materials and methods. The study was carried out on 53 Wistar rats, divided into 3 series. In each series, osteomyelitis of the femur was modeled by direct inoculation into the femoral canal of a monobacterial culture (Staphylococcus aureus; MSSA, museum strain, or Pseudomonas aeruginosa, museum strain, or Escherichia coli, museum strain) at a concentration of 1 × 108 CFU and placing an intramedullary metal implant. The animals of each series were divided into 3 groups. In group 1, no additional manipulations were performed with the animals; in groups 2 and 3, the source of infection was exposed to a direct electric current (150 mAm, 1 minute) on days 0, 2, 4 and 6, or on days 7, 9, 11 and 13. The duration of the experiment was 21 days. Results. All animals had a clinical picture characteristic of osteomyelitis, but the nature, severity and frequency of occurrence of signs of the infectious-inflammatory process in the groups were different. Conclusion. Exposure to electric current early after infection had the most pronounced antimicrobial effect against P. aeruginosa when exposure began on day 7 after infection. A less pronounced effect of electrical exposure was noted in relation to S.aureus and E.coli when exposure began on the first day after infection.

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Emodin Combined with Multiple-Low-Frequency, Low-Intensity Ultrasound To Relieve Osteomyelitis through Sonoantimicrobial Chemotherapy

Cited by 3 publications

References 42 publications

Thermosensitive hydrogel with emodin-loaded triple-targeted nanoparticles for a rectal drug delivery system in the treatment of chronic non-bacterial prostatitis

Thermosensitive hydrogel with emodin-loaded triple-targeted nanoparticles for a rectal drug delivery system in the treatment of chronic non-bacterial prostatitis

Effects of low-frequency ultrasound combined with anti-MRSA agents on the mouse model of pulmonary infection

Characteristics of the Results of Electrical Exposure When Inducing Osteomyelitis in Rats by Different Types of Patients

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