Heat stress influence the microbiota and organic acids concentration in beef cattle rumen

Sales, Gustavo; Carvalho, Beatriz Ferreira; Schwan, Rosane Freitas; Vilela, Leonardo de Figueiredo; Gionbelli, Mateus Pies; Ávila, Carla Luiza da Silva

doi:10.1016/j.jtherbio.2021.102897

Cited by 25 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 47 up-regulated proteins, most were pyruvate, phosphate dikinase (PPDK; EC: 2.7.9.1; LCI, 1970). Others were pyruvate:ferredoxin (flavodoxin) oxidoreductase (Sales et al, 2021) and pyruvate-flavodoxin oxidoreductase (Thomas et al, 2017; EC: 1.2.7.1), phosphoenolpyruvate carboxykinase (ATP; Dai et al, 2017;EC: 4.1.1.49), and so on. The description of DEPs involved in pyruvate metabolism is shown in Supplementary Table 6.…”

Section: Pyruvate Metabolismmentioning

confidence: 99%

“…Uyeno et al (2010) reported that high temperature and humidity increased the abundance of Streptococcus and decreased the abundance of Fibrobacter and Oscillospira. Sales et al (2021) showed that beef cattle under heat stress presented lower prokaryote richness, genera Flavonifractor, Treponema, and Ruminococcus, higher genera Carnobacterium. Moreover, the ruminal pH usually decreases under heat stress conditions (Tajima et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…With the rapid development of bioinformatics, more researchers are starting to use meta-omics technology to define the rumen ecosystem's ecology and its responses to changes in diet and rearing conditions (Gruninger et al, 2019). Previous studies have shown that 16S rDNA sequencing was employed to characterize the composition and diversity of rumen microbes in Holstein heifers at elevated environmental temperatures and humidity, but with little information about their function (Uyeno et al, 2010;Sales et al, 2021), while metaproteomics can provide the means to study the role of key enzymes involved in ruminant feed utilization efficiency under a particular set of conditions that dictate function. Analysis of the metaproteomes of the rumen microbial community would reveal details about microbial community activity, structure, function, and metabolic pathway transformations that are presently lacking (Hart et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary Supplementation With Creatine Pyruvate Alters Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle

Zang

Zhao

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine. It has been shown to relieve the heat stress of beef cattle by improving antioxidant activity and rumen microbial protein synthesis, but the mechanism of CrPyr influencing rumen fermentation remains unclear. This study aimed to combine 16S rDNA sequencing and metaproteomics technologies to investigate the microbial composition and function in rumen fluid samples taken from heat-stressed beef cattle treated with or without 60 g/day CrPyr. 16S rDNA sequencing revealed that there were no significant differences in the α-diversity indices between the two groups. By analyzing the level profiles of 700 distinct proteins, we found that the CrPyr administration increased the expression of enzymes involved in specific metabolic pathways including (i) fatty acid β-oxidation; (ii) interconversion from pyruvate to phosphoenolpyruvate, oxaloacetate, acetyl-CoA, and malate; (iii) glycolysis/gluconeogenesis and citrate cycle metabolism; and (iv) biosynthesis of amino acids. These results indicated that the increased generation of adenosine triphosphate during fatty acid β-oxidation or citrate cycle and the up-regulation synthesis of microbial protein in rumen of beef cattle treated with CrPyr may help decrease oxidative stress, regulate energy metabolism, and further improve the rumen fermentation characteristic under heat stress.

show abstract

Section: Pyruvate Metabolismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Supplementation With Creatine Pyruvate Alters Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle

Zang

Zhao

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In their study it was found that heat stress has no effect on both alpha and beta diversity, however the effect on the richness of microbiota was identified, especially significant increase in the abundance of Streptococcus, Enterobacteriaceae, Ruminobacter, Treponema and Bacteroidaceae . Sales and colleagues [13] in his study also reported that heat stress influenced microbiota in beef cattle rumen. Particularly, they found genera Flavonifractor, Treponema, Ruminococcus , and Carnobacterium significantly associated with heat stress.…”

Section: Introductionmentioning

confidence: 96%

Fecal microbiota and their association with heat stress in Bos taurus

Czech

Szyda

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

Humans have been influencing climate changes by burning fossil fuels, farming livestock, and cutting down rainforests, which has led to global temperature rise. This problem of global warming affects animals by causing heat stress, which negatively affects their health, biological functions, and reproduction. On the molecular level, it has been proved that heat stress changes the expression level of genes and therefore causes changes in proteome and metabolome. The importance of a microbiome in many studies showed that it is considered as individuals' 'second genome'. Physiological changes caused by heat stress may impact the microbiome composition. In this study, we identified fecal microbiota associated with heat stress that was quantified by three metrics - rectal temperature, drooling, and respiratory scores and represented by their Estimated Breeding Values. For this purpose, the 16S rRNA sequencing technique was used. We analyzed the microbiota from 136 fecal samples of Chinese Holstein cows through a 16S rRNA gene sequencing approach. Sequence data were processed using a pipeline involving QIIME2 software together with SILVA database. Statistical modeling was performed using a negative binomial regression. The analysis revealed the total number of 24 genera and 12 phyla associated with heat stress metrics. Rhizobium and Pseudobutyrivibrio turned out to be the most significant genera, while Acidobacteria and Gemmatimonadetes were the most significant phyla. Phylogenetic analysis revealed that that three heat stress indicators quantify different metabolic ways of animals' reaction to heat stress. Other studies already identified that those genera had significantly increased abundance in mice exposed to stressor-induced changes. Moreover, identified microbiota significantly associated with heat stress measures were mostly mesophilic, so their association seems to be due to heat stress-induced secondary, metabolic changes, and not directly by temperature. Moreover, high fold changes of many genera suggest that they may be used as biomarkers for monitoring the level of heat stress in cattle.

show abstract

“…In connection to this, the rumen pH of Holstein heifers decreased when the animals were maintained at environmental temperatures ranging from 20 to 33°C ( Tajima et al, 2007 ). Furthermore, cows with higher ruminal temperature values had an altered rumen microbial population ( Yadav et al, 2013 ; Correia Sales et al, 2021 ). This includes a reduction in Fibrobacter succinogenes, Flavonifractor, Prevotella ruminicola, Ruminococcus flavefaciens , and Treponema .…”

Section: Introductionmentioning

confidence: 99%

Heat Stress: Effects on Rumen Microbes and Host Physiology, and Strategies to Alleviate the Negative Impacts on Lactating Dairy Cows

et al. 2022

View full text Add to dashboard Cite

Heat stress (HS) in dairy cows causes considerable losses in the dairy industry worldwide due to reduced animal performance, increased cases of metabolic disorders, altered rumen microbiome, and other health problems. Cows subjected to HS showed decreased ruminal pH and acetate concentration and an increased concentration of ruminal lactate. Heat-stressed cows have an increased abundance of lactate-producing bacteria such as Streptococcus and unclassified Enterobacteriaceae, and soluble carbohydrate utilizers such as Ruminobacter, Treponema, and unclassified Bacteroidaceae. Cellulolytic bacteria, especially Fibrobacteres, increase during HS due to a high heat resistance. Actinobacteria and Acetobacter, both acetate-producing bacteria, decreased under HS conditions. Rumen fermentation functions, blood parameters, and metabolites are also affected by the physiological responses of the animal during HS. Isoleucine, methionine, myo-inositol, lactate, tryptophan, tyrosine, 1,5-anhydro-D-sorbitol, 3-phenylpropionic acid, urea, and valine decreased under these conditions. These responses affect feed consumption and production efficiency in milk yield, growth rate, and reproduction. At the cellular level, activation of heat shock transcription factor (HSF) (located throughout the nucleus and the cytoplasm) and increased expression of heat shock proteins (HSPs) are the usual responses to cope with homeostasis. HSP70 is the most abundant HSP family responsible for the environmental stress response, while HSF1 is essential for increasing cell temperature. The expression of bovine lymphocyte antigen and histocompatibility complex class II (DRB3) is downregulated during HS, while HSP90 beta I and HSP70 1A are upregulated. HS increases the expression of the cytosolic arginine sensor for mTORC1 subunits 1 and 2, phosphorylation of mammalian target of rapamycin and decreases the phosphorylation of Janus kinase-2 (a signal transducer and activator of transcription factor-5). These changes in physiology, metabolism, and microbiomes in heat-stressed dairy cows require urgent alleviation strategies. Establishing control measures to combat HS can be facilitated by elucidating mechanisms, including proper HS assessment, access to cooling facilities, special feeding and care, efficient water systems, and supplementation with vitamins, minerals, plant extracts, and probiotics. Understanding the relationship between HS and the rumen microbiome could contribute to the development of manipulation strategies to alleviate the influence of HS. This review comprehensively elaborates on the impact of HS in dairy cows and introduces different alleviation strategies to minimize HS.

show abstract

Heat stress influence the microbiota and organic acids concentration in beef cattle rumen

Cited by 25 publications

References 41 publications

Dietary Supplementation With Creatine Pyruvate Alters Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle

Dietary Supplementation With Creatine Pyruvate Alters Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle

Fecal microbiota and their association with heat stress in Bos taurus

Heat Stress: Effects on Rumen Microbes and Host Physiology, and Strategies to Alleviate the Negative Impacts on Lactating Dairy Cows

Contact Info

Product

Resources

About