Dietary Supplementation With Creatine Pyruvate Alters Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle

Abstract

Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine. It has been shown to relieve the heat stress of beef cattle by improving antioxidant activity and rumen microbial protein synthesis, but the mechanism of CrPyr influencing rumen fermentation remains unclear. This study aimed to combine 16S rDNA sequencing and metaproteomics technologies to investigate the microbial composition and function in rumen fluid samples taken from heat-stressed beef cattle treated with or without 60 g/day CrPyr. 16S rDNA sequencing revealed that there were no significant differences in the α-diversity indices between the two groups. By analyzing the level profiles of 700 distinct proteins, we found that the CrPyr administration increased the expression of enzymes involved in specific metabolic pathways including (i) fatty acid β-oxidation; (ii) interconversion from pyruvate to phosphoenolpyruvate, oxaloacetate, acetyl-CoA, and malate; (iii) glycolysis/gluconeogenesis and citrate cycle metabolism; and (iv) biosynthesis of amino acids. These results indicated that the increased generation of adenosine triphosphate during fatty acid β-oxidation or citrate cycle and the up-regulation synthesis of microbial protein in rumen of beef cattle treated with CrPyr may help decrease oxidative stress, regulate energy metabolism, and further improve the rumen fermentation characteristic under heat stress.

Chemical Group-Based Metabolome Analysis Identifies Candidate Plasma Biomarkers Associated With Residual Feed Intake in Beef Steers

“…In addition to the role of methionine in tissue protein synthesis, methionine can serve as a precursor for synthesis of other amino acids such as taurine, cysteine, apolipoprotein, and can donate its methyl via S-adenosyl methionine, for synthesis of choline, carnitine, creatine, and phospholipids which are all essential for improved skeletal muscle and hepatic lipid metabolism for energy supply especially during reduced supply of glucose. In this study, lower plasma level of methionine in HFE steers was accompanied with higher plasma levels of taurine and creatine, which are known to regulate lipid metabolism in humans and farm animals (da Silva et al, 2014;Karisa et al, 2014;Ibrahim et al, 2019;Li et al, 2021). In a similar study, Karisa et al (2014) reported that plasma concentration of creatine was associated with RFI.…”

In Vitro Evaluation of Chito-Oligosaccharides on Disappearance Rate of Nutrients, Rumen Fermentation Parameters, and Micro-Flora of Beef Cattle

“…In previous studies, people have tried almost all feed substitutes and achieved remarkable results, as well as tried different types of additives, which have been proven to improve animal growth performance improving growth and enhancing immunity [7][8][9][10].…”

“…In previous studies, people have tried almost all feed substitutes and achieved remarkable results, as well as tried different types of additives, which have been proven to improve animal growth performance improving growth and enhancing immunity [ 7 , 8 , 9 , 10 ]. Chitin is a natural polymer with high production and biodegradability in nature, second only to cellulose.…”