Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture

Abstract

Plant growth promoting microbes (PGPMs) play major roles in diverse ecosystems, including atmospheric nitrogen fixation, water uptake, solubilization, and transport of minerals from the soil to the plant. Different PGPMs are proposed as biofertilizers, biostimulants, and/or biocontrol agents to improve plant growth and productivity and thereby to contribute to agricultural sustainability and food security. However, little information exists regarding the use of PGPMs in micropropagation such as the in vitro plant tissue culture. This review presents an overview of the importance of PGPMs and their potential application in plant micropropagation. Our analysis, based on published articles, reveals that the process of in vitro classical tissue culture techniques, under strictly aseptic conditions, deserves to be reviewed to allow vitroplants to benefit from the positive effect of PGPMs. Furthermore, exploiting the potential benefits of PGPMs will lead to lessen the cost production of vitroplants during micropropagation process and will make the technique of plant tissue culture more efficient. The last part of the review will indicate where research is needed in the future.

Biostimulants to Improved Tree Physiology and Fruit Quality: A Review with Special Focus on Sweet Cherry

“…These saprotrophic fungi can promote plant growth, by producing antimicrobial compounds, by parasitizing other fungal pathogens, but also by increasing solubility of some soil micronutrients or by producing indole-3-acetic acid or auxin analogs [6,[78][79][80][81]. However, plants can present interaction with a wide variety of soil fungi, from various taxa, belonging to arbuscular mycorrhizal fungi, orchid mycorrhizal fungi, ericoid mycorrhizal fungi, ectomycorrhizal fungi, Piriformospora, and other root endophytes fungi such as Fusarium spp., Penicillium spp., and Aspergillus spp., all recognized as being plant growth-promoting fungi [82]. Arbuscular mycorrhizal fungi (AMF) are known to have a major role in plant nutrition, as they can improve mineral uptake by plants [83] and are widely used in horticulture, namely Rhizophagus (formerly known as Glomus) intraradices and Funneliformis (formerly known as Glomus) mosseae [84].…”

Biotization of Endophytes Trichoderma asperellum and Bacillus subtilis in Mentha spicata Microplants to Promote Growth, Pathogen Tolerance and Specialized Plant Metabolites

“…Conventionally, micropropagation techniques are carried out under completely aseptic conditions. However, due to the current knowledge on the role of endophytic microorganisms as growth promoters and inducers of pest tolerance, importance is being given to their use in some of the stages of micropropagation such as proliferation, rooting and acclimatization, which is known as biotization [ 8 ].…”

Soil Microbes Determine Outcomes of Pathogenic Interactions Between Radopholus similis and Fusarium oxysporum V5w2 in Tissue Culture Banana Rhizospheres Starved of Nitrogen, Phosphorus, and Potassium

“…Aseptic tissue culture protocols target the elimination of complexes of non-beneficial endophytic and soil-derived biota that are associated with plant growth suppression (Cassells, 2012;. Endophytic and rhizosphere plant growth promoting microbes are re-introduced to restore their beneficial functions lost through aseptic tissue culture and soil sterilization techniques (Niere et al, 1999;Sikora and Pocasangre, 2006;Sikora et al, 2008;Soumare et al, 2021). For instance, Fusarium oxysporum strain V5w2 is among endophytic microbes that have successfully been re-introduced into tissue culture banana plants and transferred to farmers in East Africa (Dubois et al, 2006(Dubois et al, , 2011zum Felde, 2011).…”