Alleviation of colonic inflammation by Lypd8 in a mouse model of inflammatory bowel disease

Hsu, Chiao-Ching; Okumura, Ryu; Motooka, Daisuke; Sasaki, Reo; Nakamura, Shota; Iida, Tetsuya; Takeda, Kiyoshi

doi:10.1093/intimm/dxab012

Cited by 8 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lypd8 −/− mice show spontaneous inflammatory response than normal mice or high‐fat diet (HFD) mice. 34 Lypd8 gene is selectively expressed in the uppermost epithelial cells of the colorectal glands, possibly. It is in the Ly6/PLAUR domain containing eight (Lypd8) proteins, which can bind to the flagella of gram‐negative bacilli or Proteus mirabilis to inhibit the movement of the flagella, thereby preventing bacteria from entering the inner mucus layer, inhibiting the inflammatory response of epithelial cells, and separating bacteria and epithelial cell layer.…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have found that if Lypd8 gene expression is blocked, bacteria can directly bind to receptors on the cell membrane. Lypd8 −/− mice show spontaneous inflammatory response than normal mice or high‐fat diet (HFD) mice 34 . Lypd8 gene is selectively expressed in the uppermost epithelial cells of the colorectal glands, possibly.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Research progress on the relationship between intestinal microecology and intestinal bowel disease

Song

et al. 2022

Anim Models and Exp Med

View full text Add to dashboard Cite

Intestinal microecology is the main component of human microecology. Intestinal microecology consists of intestinal microbiota, intestinal epithelial cells, and intestinal mucosal immune system. These components are interdependent and establish a complex interaction network that restricts each other. According to the impact on the human body, there are three categories of symbiotic bacteria, opportunistic pathogens, and pathogenic bacteria. The intestinal microecology participates in digestion and absorption, and material metabolism, and inhibits the growth of pathogenic microorganisms. It also acts as the body's natural immune barrier, regulates the innate immunity of the intestine, controls the mucosal barrier function, and also participates in the intestinal epithelial cells' physiological activities such as hyperplasia or apoptosis. When the steady-state balance of the intestinal microecology is disturbed, the existing core intestinal microbiota network changes and leads to obesity, diabetes, and many other diseases, especially irritable bowel syndrome, inflammatory bowel disease (IBD), and colorectal malignancy. Intestinal diseases, including tumors, are particularly closely related to intestinal microecology. This article systematically discusses the research progress on the relationship between IBD and intestinal microecology from the pathogenesis, treatment methods of IBD, and the changes in intestinal microbiota.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research progress on the relationship between intestinal microecology and intestinal bowel disease

Song

et al. 2022

Anim Models and Exp Med

View full text Add to dashboard Cite

show abstract

“…Thus, the high expression of SLC26A3 could prevent DSS−induced acute colitis [ 58 ]. LYPD8 levels are significantly decreased in colitis patients, and supplementation of LYPD8 proteins might maintain colonic homeostasis in colitis patients by inhibiting the motility of pathogenic bacteria, such as γ-proteobacteria [ 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Milk-Derived Extracellular Vesicles on the Colonic Transcriptome and Proteome in Murine Model

Zhao

Wang

et al. 2022

Nutrients

View full text Add to dashboard Cite

Evidence shows that effective nutritional intervention can prevent or mitigate the risk and morbidity of inflammatory bowel disease (IBD). Bovine milk extracellular vesicles (mEVs), a major bioactive constituent of milk, play an important role in maintaining intestinal health. The aims of this study were to assess the effects of mEV pre-supplementation on the colonic transcriptome and proteome in dextran sulphate sodium (DSS)-induced acute colitis, in order to understand the underlying molecular mechanisms of mEV protection against acute colitis. Our results revealed that dietary mEV supplementation alleviated the severity of acute colitis, as evidenced by the reduced disease activity index scores, histological damage, and infiltration of inflammatory cells. In addition, transcriptome profiling analysis found that oral mEVs significantly reduced the expression of pro-inflammatory cytokines (IL-1β, IL-6, IL-17A and IL-33), chemokine ligands (CXCL1, CXCL2, CXCL3, CXCL5, CCL3 and CCL11) and chemokine receptors (CXCR2 and CCR3). Moreover, oral mEVs up-regulated 109 proteins and down-regulated 150 proteins in the DSS-induced murine model, which were involved in modulating amino acid metabolism and lipid metabolism. Collectively, this study might provide new insights for identifying potential targets for the therapeutic effects of mEVs on colitis.

show abstract

“…Prior to FISH, the sections were treated with TrueBlack (Biotium Inc. Hayward, CA, USA) to reduce nonspecific fluorescence. Hybridization was conducted as described previously ( Hsu et al., 2021 ). Sections were incubated overnight at 40 °C with EUB338 probe or NON338.…”

Section: Methodsmentioning

confidence: 99%

Localization and potential role of prostate microbiota

Okada

Takezawa

Tsujimura

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

IntroductionWe aimed to clarify the presence and localization of the prostate microbiota and examine its association with benign prostate enlargement (BPE).MethodsThe microbiota of prostate tissues and catheterized urine from 15 patients were analyzed by 16S metagenomic analysis and compared to show that the prostate microbiota was not a contaminant of the urinary microbiota. Fluorescence in situ hybridization (FISH) and in situ hybridization (ISH) using the specific probe for eubacteria was performed on prostate tissue to show the localization of bacteria in the prostate. The BPE group was defined as prostate volume ≥30 mL, and the non-BPE group as prostate volume <30 mL. The microbiota of the two groups were compared to clarify the association between prostate microbiota and BPE.ResultsFaith’s phylogenetic diversity index of prostate tissue was significantly higher than that of urine (42.3±3.8 vs 25.5±5.6, P=0.01). Principal coordinate analysis showed a significant difference between the microbiota of prostate tissue and catheterized urine (P<0.01). FISH and ISH showed the presence of bacteria in the prostatic duct. Comparison of prostate microbiota between the BPE and non-BPE groups showed that the Chao1 index of the BPE group was significantly lower than that of the latter [142 (50–316) vs 169 (97–665), P=0.047] and the abundance of Burkholderia was significantly higher in the BPE group than in the latter.ConclusionsWe demonstrated that the prostate microbiota was located in the prostatic duct and reduced diversity of prostate microbiota was associated with BPE, suggesting that prostate microbiota plays a role in BPE.

show abstract

Alleviation of colonic inflammation by Lypd8 in a mouse model of inflammatory bowel disease

Cited by 8 publications

References 49 publications

Research progress on the relationship between intestinal microecology and intestinal bowel disease

Research progress on the relationship between intestinal microecology and intestinal bowel disease

Effects of Milk-Derived Extracellular Vesicles on the Colonic Transcriptome and Proteome in Murine Model

Localization and potential role of prostate microbiota

Contact Info

Product

Resources

About