Selective enrichment of comammox from activated sludge using antibiotics

Wang, Yulin; Zhao, Renxin; Liu, Lei; Li, Bing; Zhang, Tong

doi:10.1016/j.watres.2021.117087

Cited by 37 publications

(9 citation statements)

References 61 publications

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“…Nitrospira sp. LK70 and canonical NOB, Nitrospira moscoviensis ( Yang et al, 2020 ; Wang Y. et al, 2021 ) but comammox Nitrospira spp. consist of high diversity in urea transporters genes and lacks in cynS , indicating a considerable genome difference compared to canonical Nitrospira NOB ( Palomo et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Hsu

Cameron

et al. 2022

Front. Microbiol.

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The recent discovery of comammox Nitrospira, a complete ammonia oxidizer, capable of completing the nitrification on their own has presented tremendous challenges to our understanding of the nitrification process. There are two divergent clades of comammox Nitrospira, Clade A and B. However, their population abundance, community structure and role in ammonia and nitrite oxidation are poorly understood. We conducted a 94-day microcosm study using a grazed dairy pasture soil amended with urea fertilizers, synthetic cow urine, and the nitrification inhibitor, dicyandiamide (DCD), to investigate the growth and community structure of comammox Nitrospira spp. We discovered that comammox Nitrospira Clade B was two orders of magnitude more abundant than Clade A in this fertile dairy pasture soil and the most abundant subcluster was a distinctive phylogenetic uncultured subcluster Clade B2. We found that comammox Nitrospira Clade B might not play a major role in nitrite oxidation compared to the role of canonical Nitrospira nitrite-oxidizers, however, comammox Nitrospira Clade B is active in nitrification and the growth of comammox Nitrospira Clade B was inhibited by a high ammonium concentration (700 kg synthetic urine-N ha–1) and the nitrification inhibitor DCD. We concluded that comammox Nitrospira Clade B: (1) was the most abundant comammox in the dairy pasture soil; (2) had a low tolerance to ammonium and can be inhibited by DCD; and (3) was not the dominant nitrite-oxidizer in the soil. This is the first study discovering a new subcluster of comammox Nitrospira Clade B2 from an agricultural soil.

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Section: Discussionmentioning

confidence: 99%

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Hsu

Cameron

et al. 2022

Front. Microbiol.

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“…The comammox bacteria metabolism pathway is complex and can adapt to different nutrient substrates (Daims et al, 2015;Van Kessel et al, 2015;Shen et al, 2016;Kits et al, 2017;Han et al, 2019;Liu et al, 2020a;Wang et al, 2021;Zhao et al, 2021). Liu et al (2020a) found that comammox bacteria contribute 34%-87% of nitrification in a typical oligotrophic environment with high pH and low temperature along the Yangtze River.…”

Section: Enzymes and Metabolismmentioning

confidence: 99%

“…Studies have found that comammox bacteria can use hydrogen sulfide, sulfide, and fermentation products for metabolism (Kits et al, 2017), while trace organic pollutants in the environment can be removed through metabolism (Han et al, 2019). Some comammox may also have the potential to oxidize formic acid and hydrogen (Palomo et al, 2018), and a variety of antibiotics have been used to effectively and selectively enrich different strains of comammox bacteria (Wang et al, 2021). In addition, comammox bacteria have a gene encoding alkaline phosphatase, which can make it more advantageous under insufficient phosphorus conditions (Shen et al, 2016).…”

Section: Enzymes and Metabolismmentioning

confidence: 99%

Research progress and prospects of complete ammonia oxidizing bacteria in wastewater treatment

Luo

Peng²,

Liu

et al. 2022

Front. Environ. Sci. Eng.

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Complete ammonia oxidizing bacteria, or comammox bacteria (CAOB), can oxidize ammonium to nitrate on its own. Its discovery revolutionized our understanding of biological nitrification, and its distribution in both natural and artificial systems has enabled a reevaluation of the relative contribution of microorganisms to the nitrogen cycle. Its wide distribution, adaptation to oligotrophic medium, and diverse metabolic pathways, means extensive research on CAOB and its application in water treatment can be promoted. Furthermore, the energy-saving characteristics of high oxygen affinity and low sludge production may also become frontier directions for wastewater treatment. This paper provides an overview of the discovery and environmental distribution of CAOB, as well as the physiological characteristics of the microorganisms, such as nutrient medium, environmental factors, enzymes, and metabolism, focusing on future research and the application of CAOB in wastewater treatment. Further research should be carried out on the physiological characteristics of CAOB, to analyze its ecological niche and impact factors, and explore its application potential in wastewater treatment nitrogen cycle improvement.

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“…In this process, ammonia can be completely oxidized to nitrate in a single cell via the subsequent activity of the enzyme complexes, ammonia monooxygenase, hydroxylamine dehydrogenase, and nitrite oxidoreductase (Daims et al, 2015; Kits et al, 2019; Palomo et al, 2018; van Kessel et al, 2015). To date, numerous comammox bacteria have been detected in various terrestrial and aquatic ecosystems (Shi et al, 2020; Wang, Wang, et al, 2021; Wang, Zhao, et al, 2021; Zhu et al, 2022). Nevertheless, the contribution of comammox to ammonia oxidation as well as ammonia fertilizer loss and the global N 2 O budget in agricultural soils ecosystems, significantly affecting and potentially severely undermining global food security and climate change, remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N₂O production

Jiang

Wang

et al. 2023

Global Change Biology

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The contribution of agriculture to the sustainable development goals requires climate‐smart and profitable farm innovations. Increasing the ammonia fertilizer applications to meet the global food demands results in high agricultural costs, environmental quality deterioration, and global warming, without a significant increase in crop yield. Here, we reported that a third microbial ammonia oxidation process, complete ammonia oxidation (comammox), is contributing to a significant ammonia fertilizer loss (41.9 ± 4.8%) at the rate of 3.53 ± 0.55 mg N kg−1 day−1 in agricultural soils around the world. The contribution of comammox to ammonia fertilizer loss, occurring mainly in surface agricultural soil profiles (0–0.2 m), was equivalent to that of bacterial ammonia oxidation (48.6 ± 4.5%); both processes were significantly more important than archaeal ammonia oxidation (9.5 ± 3.6%). In contrast, comammox produced less N2O (0.98 ± 0.44 μg N kg−1 day−1, 11.7 ± 3.1%), comparable to that produced by archaeal ammonia oxidation (16.4 ± 4.4%) but significantly lower than that of bacterial ammonia oxidation (72.0 ± 5.1%). The efficiency of ammonia conversion to N2O by comammox (0.02 ± 0.01%) was evidently lower than that of bacterial (0.24 ± 0.06%) and archaeal (0.16 ± 0.04%) ammonia oxidation. The comammox rate increased with increasing soil pH values, which is the only physicochemical characteristic that significantly influenced both comammox bacterial abundance and rates. Ammonia fertilizer loss, dominated by comammox and bacterial ammonia oxidation, was more intense in soils with pH >6.5 than in soils with pH <6.5. Our results revealed that comammox plays a vital role in ammonia fertilizer loss and sustainable development in agroecosystems that have been previously overlooked for a long term.

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Selective enrichment of comammox from activated sludge using antibiotics

Cited by 37 publications

References 61 publications

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Research progress and prospects of complete ammonia oxidizing bacteria in wastewater treatment

Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N₂O production

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Selective enrichment of comammox from activated sludge using antibiotics

Cited by 37 publications

References 61 publications

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Research progress and prospects of complete ammonia oxidizing bacteria in wastewater treatment

Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N2O production

Contact Info

Product

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Complete ammonia oxidization in agricultural soils: High ammonia fertilizer loss but low N₂O production