Antioxidant and antifatigue effect of a standardized fraction (HemoHIM) from Angelica gigas , Cnidium officinale , and Paeonia lactiflora

Abstract

Context HemoHIM is an herbal preparation containing Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia lactiflora Pallas (Paeoniaceae) developed for immune regulation. To date, studies on the antifatigue effects of HemoHIM have not been conducted. Objective The antifatigue effects of HemoHIM using models of citrinin and exercise-induced chronic fatigue syndrome were investigated. Materials and methods Citrinin-induced L6 skeletal muscle cells were treated with HemoHIM (125, 250, and 500 μg/mL). The antioxidant factors were analysed. ICR mice were divided into four groups (n = 10): control, HemoHIM 250, 500 mg/kg, and creatine 300 mg/kg, respectively. Mice were orally administered HemoHIM or creatine for three weeks; during this time, both rotarod test and forced swimming test (FST) were conducted. The latency time was investigated and antioxidant, antifatigue factors were analysed. Results HemoHIM significantly restored reduced antioxidant enzymes (SOD, CAT, Txn, GPx, GSr, and GCLC in HemoHIM 500 μg/mL) compared to the citrinin group in L6 cells. In vivo , HemoHIM significantly improved the latency time (FST; 279.88 ± 50.32 sec, rotarod test; 552.35 ± 23.50 sec in HemoHIM 500 mg/kg). Moreover, the FST-induced reduction in glucose and glutathione significantly increased by 3-fold (HemoHIM 500 mg/kg) and increase in LDH and MDA were significantly inhibited by 1.6, 2.1-fold in the HemoHIM 500 mg/kg compared to the control group.

The Emerging Role of Gut Microbiota in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Current Evidence and Potential Therapeutic Applications

“…In this respect, apple polysaccharides, flos lanicera administration and Bofutsushasan (a Japanese herbal medicine) have proven effective in favoring Lactobacillus and Bacteroidetes growth, enhancing tight junction function and reducing the pro-inflammatory cytokines TNF-α and IL-6 [ 157 , 158 , 159 ]. Additionally, integration of Sarcodon imbricatus or intake of a mixture composed of Angelica gigas , Cnidium officinale , and Paeonia lactiflora , proved effective in restoring the oxidant/antioxidant homeostasis and in reducing fatigue in ME/CFS mouse models [ 160 , 161 ].…”

Ginsenoside Rg1 can reverse fatigue behavior in CFS rats by regulating EGFR and affecting Taurine and Mannose 6-phosphate metabolism

“…The rotarod test is widely used to assess athletic endurance with high reproducibility. In fact, the improvement of exercise tolerance is the strongest evidence for the existence of anti-fatigue effects ( Kwon et al, 2021 ). In this study, CFS rats showed a decline in exercise tolerance that could be reversed by ginsenoside Rg1.…”

Phytochemical Contain, Biological Properties and Toxicities of Herbals Used for Doping

“…Bioactive compounds present in herbal material exist as multicomponent mixtures. Characterization, analysis, separation and identification of bioactive phytoconstituents that may be at the origin of the anti-fatigue potential of herbal involves approaches which the application of common phytochemical screening assays, chromatographic and spectrometrique techniques [23,117,118]. The development of applied untargeted metabolomic assays presents a new direction for the discovery of metabolite indicators for sport and exercise science [119].…”