“…Autophagy suppression enhances the invasiveness of the trophoblastic cell lines and NF-κB activity, but NF-κB inhibitor attenuates the trophoblast invasion (Oh et al, 2020). In addition, normal morula embryos and tumour necrosis factor α (TNF-α)-treated morula embryos have differential effects on the expression of genes and proteins in uterine tissues and spleen through NF-κB pathway during preimplantation pregnancy in mice (Buska-Mach et al, 2021). It is unclear whether the expression of NF-κB components in the spleen is changed during early pregnancy in ewes.…”