Changes in nuclear factor kappa B components expression in the ovine spleen during early pregnancy

Hao, Shuai; Fang, H.; Fang, Shengya; Zhang, T.; Zhang, L.; Yang, Ling

doi:10.22358/jafs/146491/2022

Cited by 8 publications

(18 citation statements)

References 41 publications

(41 reference statements)

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“…BCL-3 is involved in the immune response through interactions with the NF-κB subunits, and plays essential roles in germinal center formation and marginal zone B-cell development [ 36 , 37 ]. There is an upregulation of NF-κB1 or NF-κB2 in the maternal spleen during early pregnancy [ 7 ], suggesting that BCL-3 participates in the regulation of adaptive immunity through binding homodimers of NF-κB1 or NF-κB2. BCL-3 is required for dendritic cells in effective priming of CD4 and CD8 T cells and generates adaptive immunity in mice [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…The samples of spleens and lymph nodes were prepared as described previously [ 7 , 9 ], and total proteins were separated on polyacrylamide gels. Total proteins were transferred to PVDF membranes (Millipore, Bedford, MA, USA), and the membranes were incubated with primary antibodies overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Nuclear factor kappa B (NF-κB) is implicated in both physiological and pathological processes; NF-κB regulation is essential during pregnancy, and dysregulation results in premature termination of pregnancy, with bad outcomes for the mother and the fetus [ 5 ]. Early pregnancy modulates the expression of NF-κB components in the maternal thymus, spleen, liver and inguinal lymph nodes, which is necessary for embryo implantation and pregnancy maintenance in sheep [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies report that interferon-stimulated genes (ISGs), progesterone receptor, progesterone-induced blocking factor, tumor necrosis factor (TNF)-β, IL-2, IL-5, IL-6, IL-10, cyclooxygenase 2, aldo-keto reductase family 1, member B1, melatonin receptor 1 (MT1), gonadotropin-releasing hormone and its receptor are upregulated, but MT2 is downregulated in the maternal spleen of ewes during early pregnancy [ 21 , 22 , 23 , 24 , 25 , 26 ]. In addition, early pregnancy modulates the expression of Toll-like receptor signaling members and NF-κB components in the ovine maternal spleen [ 7 , 27 ]. Furthermore, the expression of ISGs, progesterone receptor, prostaglandin synthases, Th cytokines, MT1, gonadotropin-releasing hormone and its receptor, and prolactin and its receptor are changed in lymph nodes during early pregnancy in sheep [ 24 , 25 , 28 , 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes

Fang

Cai

Bai

et al. 2023

IJMS

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Early pregnancy modulates the maternal immune system, including the spleen and lymph nodes, which participate in maternal innate and adaptive immune responses. Methods: Ovine spleens and lymph nodes were sampled at day 16 of the estrous cycle, and at days 13, 16 and 25 of gestation, and qRT-PCR, Western blot and immunohistochemistry analysis were used to analyze the expression of the IκB family, including BCL-3, IκBα, IκBβ, IκBε, IKKγ, IκBNS and IκBζ. Early pregnancy induced expression of BCL-3, IκBα, IκBε, IKKγ and IκBζ, and expression of BCL-3, IκBβ and IκBNS peaked at day 16 of pregnancy in the spleen. However, early pregnancy suppressed the expression of BCL-3 and IκBNS, but stimulated the expression of IκBβ and IκBζ, and expression levels of IκBα, IκBβ, IκBε and IKKγ peaked in lymph nodes at days 13 and/or 16 of pregnancy. Early pregnancy changed the expression of the IκB family in the maternal spleen and lymph node in a tissue-specific manner, suggesting that the modulation of the IκB family may be involved in regulation of maternal functions of the spleen and lymph nodes, which are necessary for the establishment of maternal immune tolerance during early pregnancy in sheep.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes

Fang

Cai

Bai

et al. 2023

IJMS

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“…In addition, expression of melatonin receptor 1, CD4, gonadotropin releasing hormone (GnRH) and GnRH receptor is changed in maternal inguinal lymph nodes during early pregnancy in sheep [ 19 , 20 ]. It is suggested that early pregnancy may have effects on immunoregulatory signaling pathway, for example nuclear factor kappa B (NF-κB) pathway [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy

Zhang

Yang

et al. 2022

BMC Vet Res

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Background Pregnancy-induced immunological changes contribute to the maternal immune tolerance. Nuclear factor kappa B (NF-κB) pathway participates in regulating both innate and adaptive immunities, and lymph nodes play key roles in adaptive immune reaction. However, it is unclear whether early pregnancy changes the expression of NF-κB family in maternal lymph node in sheep. Methods In this study, the samples of inguinal lymph nodes were collected from ewes on day 16 of the estrous cycle, and on days 13, 16 and 25 of pregnancy, and expression of NF-κB family, including NF-κB p105 (NFKB1), NF-κB p100 (NFKB2), p65 (RELA), RelB (RELB) and c-Rel (REL), were analyzed through real-time quantitative PCR, Western blot and immunohistochemical analysis. Results The expression levels of NF-κB p105 and c-Rel downregulated, but NF-κB p100 upregulated on day 25 of pregnancy. The expression levels of p65, RelB and c-Rel peaked at day 13 of pregnancy, and expression level of RelB was higher during early pregnancy comparing to day 16 of the estrous cycle. In addition, p65 protein was located in the subcapsular sinus and lymph sinuses. Conclusion This paper reported for the first time that early pregnancy has effects on the expression of NF-κB family, which may contribute to the maternal immunoregulation through blood circulation and lymph circulation during early pregnancy in sheep.

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Changes in expression levels of Nod-like receptors in the spleen of ewes

Fang

Feng

et al. 2023

Anim. Reprod.

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Nucleotide-binding oligomerization domain receptors (NOD-like receptors, NLRs) have critical effects on interfaces of the immune and reproductive systems, and the spleen plays a key role in both innate and adaptive immune functions. It is hypothesized that NLR family participates in maternal splenic immune regulation during early pregnancy in sheep. In this study, maternal spleens were collected on day 16 of the estrous cycle, and days 13, 16 and 25 of gestation (n = 6 for each group) in ewes. Expression of NLR family, including NOD1, NOD2, class II transactivator (CIITA), NLR family apoptosis inhibitory protein (NAIP), nucleotide-binding oligomerization domain, Leucine rich repeat and Pyrin domain containing 1 (NLRP1), NLRP3 and NLRP7, was analyzed using quantitative real-time PCR, Western blot and immunohistochemistry analysis. The results revealed that expression levels of NOD1, NOD2, CIITA and NLRP3 were downregulated at days 13 and 16 of pregnancy, but expression of NLRP3 was increased at day 25 of pregnancy. In addition, expression values of NAIP and NLRP7 mRNA and proteins were improved at days 16 and 25 of pregnancy, and NLRP1 was peaked at days 13 and 16 of pregnancy in the maternal spleen. Furthermore, NOD2 and NLRP7 proteins were limited to the capsule, trabeculae and splenic cords. In summary, early pregnancy changes expression of NLR family in the maternal spleen, which may be related with the maternal splenic immunomodulation during early pregnancy in sheep.

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Changes in nuclear factor kappa B components expression in the ovine spleen during early pregnancy

Cited by 8 publications

References 41 publications

Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes

Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes

Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy

Changes in expression levels of Nod-like receptors in the spleen of ewes

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