Isolation and characterization of
            <i>Helicobacter suis</i>
            from human stomach

Rimbara, Emiko; Suzuki, Masato; Matsui, Hidenori; Nakamura, Masahiko; Morimoto, Misako; Sasakawa, Chihiro; Masuda, Hiroki; Nomura, Sachiyo; Osaki, Takako; Nagata, Noriyo; Shibayama, Keigo; Tokunaga, Kazutoshi

doi:10.1073/pnas.2026337118

Cited by 22 publications

(51 citation statements)

References 31 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to what has been clearly demonstrated for H. pylori , some H. suis strains might be more pathogenic than others [ 47 ]. To this end, multilocus sequence typing (MLST) of H. suis strains has been executed, but a possible relationship between MLST type and virulence has not been demonstrated yet [ 48 ].…”

Section: Pathogenic Significance Prevalence and Transmission Of ...supporting

confidence: 75%

“…For H. suis , an additional means of transmission may include consumption of contaminated pork [ 100 ]. These means of direct and indirect transmission are further validated by reports of molecular analyses proving the great genetic similarity of gastric NHPH strains present in humans and these animal hosts [ 8 , 47 , 61 , 93 , 94 ]. Furthermore, since laboratory mice are easily colonized by most gastric NHPH, the role of wild mice as a vector for the transmission of gastric NHPH should also be considered.…”

Section: Zoonotic Potential Of Gastric Non- Helicobacter Py...mentioning

confidence: 83%

“…Helicobacter felis has also been isolated from a patient presenting with acute abdominal pain, nausea and vomiting of brown fluid, symptoms that appeared to be related to severe acute gastritis [ 90 ]. Only recently, in 2020, Rimbara et al succeeded in obtaining pure cultures of H. suis strains from three human patients with gastric diseases, including gastric MALT lymphoma [ 47 , 91 ]. Isolation and cultivation of other gastric NHPH from human patients with gastric diseases has not yet been described.…”

Section: Zoonotic Potential Of Gastric Non- Helicobacter Py...mentioning

confidence: 99%

See 2 more Smart Citations

Gastric Helicobacter species associated with dogs, cats and pigs: significance for public and animal health

Taillieu

Chiers

Amorim

et al. 2022

Vet Res

View full text Add to dashboard Cite

This article focuses on the pathogenic significance of Helicobacter species naturally colonizing the stomach of dogs, cats and pigs. These gastric “non-Helicobacter (H.) pylori Helicobacter species” (NHPH) are less well-known than the human adapted H. pylori. Helicobacter suis has been associated with gastritis and decreased daily weight gain in pigs. Several studies also attribute a role to this pathogen in the development of hyperkeratosis and ulceration of the non-glandular stratified squamous epithelium of the pars oesophagea of the porcine stomach. The stomach of dogs and cats can be colonized by several Helicobacter species but their pathogenic significance for these animals is probably low. Helicobacter suis as well as several canine and feline gastric Helicobacter species may also infect humans, resulting in gastritis, peptic and duodenal ulcers, and low-grade mucosa-associated lymphoid tissue lymphoma. These agents may be transmitted to humans most likely through direct or indirect contact with dogs, cats and pigs. Additional possible transmission routes include consumption of water and, for H. suis, also consumption of contaminated pork. It has been described that standard H. pylori eradication therapy is usually also effective to eradicate the NHPH in human patients, although acquired antimicrobial resistance may occasionally occur and porcine H. suis strains are intrinsically less susceptible to aminopenicillins than non-human primate H. suis strains and other gastric Helicobacter species. Virulence factors of H. suis and the canine and feline gastric Helicobacter species include urease activity, motility, chemotaxis, adhesins and gamma-glutamyl transpeptidase. These NHPH, however, lack orthologs of cytotoxin-associated gene pathogenicity island and vacuolating cytotoxin A, which are major virulence factors in H. pylori. It can be concluded that besides H. pylori, gastric Helicobacter species associated with dogs, cats and pigs are also clinically relevant in humans. Although recent research has provided better insights regarding pathogenic mechanisms and treatment strategies, a lot remains to be investigated, including true prevalence rates, exact modes of transmission and molecular pathways underlying disease development and progression.

show abstract

Section: Pathogenic Significance Prevalence and Transmission Of ...supporting

confidence: 75%

Section: Zoonotic Potential Of Gastric Non- Helicobacter Py...mentioning

confidence: 83%

Section: Zoonotic Potential Of Gastric Non- Helicobacter Py...mentioning

confidence: 99%

See 1 more Smart Citation

Gastric Helicobacter species associated with dogs, cats and pigs: significance for public and animal health

Taillieu

Chiers

Amorim

et al. 2022

Vet Res

View full text Add to dashboard Cite

show abstract

“…In recent years, the importance of non- Helicobacter pylori Helicobacter (NHPH) is increasing due to advances in culturing techniques and genetic analysis for NHPH 1 . Eradication for Helicobacter pylori is also contributing to a focus on gastric diseases caused by NHPH.…”

Section: Introductionmentioning

confidence: 99%

Non-Helicobacter pylori Helicobacter (NHPH) positive gastric cancer

Yasuda

Lee

Nam

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are progressing. NHPH is reported to accompany nodular gastritis, gastric MALT lymphoma, and mild gastritis. However, only a few gastric cancer cases infected by NHPH have been reported. PCR analysis specific for NHPH and H. pylori was performed for DNA from gastric mucosa of 282 Korean gastric cancer patients, who were treated with endoscopic submucosal dissection. For more precise strain detection of NHPH, NHPH-positive mucosa was stained by immunohistochemistry specific for Helicobacter suis. The Cancer Genome Atlas (TCGA) classification was analyzed for these 3 gastric cancer sub-groups by in situ hybridization and immunohistochemistry. Among 281 patients, 3 patients (1.1%) were positive for NHPH. One patient (Patient 1) was also positive for H. pylori by PCR, another patient (Patient 3) was positive for serum IgG for H. pylori, and the other patient (Patient 2) had no evidence for H. pylori infection. Gastric mucosa of Patients 2 and 3 were positive for H. suis staining. All three NHPH-positive gastric cancers were located in the antrum, and belonged to the Chromosomal Instability Type of TCGA classification. Gastric NHPH can be a cause of gastric cancer, although likely with lower pathogenesis than H. pylori.

show abstract

“…4 However, a diagnostic test for NHPH infection has not been established clinically. An in vitro culture of H. suis has recently been reported 5,6 ; however, this has not yet been put into clinical use. No diagnostic method has been established for rapid urease tests, urea breath tests, blood antibody analysis, immunohistological analysis, or fecal antigen analysis, 7 and the gold standard for diagnosis has been polymerase chain reaction (PCR).…”

Section: Introductionmentioning

confidence: 99%

Investigation of endoscopic findings in nine cases of Helicobacter suis‐infected gastritis complicated by gastric mucosa‐associated lymphoid tissue lymphoma

et al. 2022

View full text Add to dashboard Cite

Background:We have previously reported that eradication therapy was more effective against Helicobacter pylori (Hp)-negative gastric mucosa-associated lymphoid tissue (MALT) lymphoma in non-Helicobacter pylori Helicobacter (NHPH)-positive cases than in NHPH-negative cases and that the infection status of NHPH could be a predictive marker for the efficacy of eradication therapy for H. pylori negative gastric MALT lymphoma. However, a diagnostic test for NHPH infection has not yet been clinically established. In this study, we investigated the endoscopic findings in cases of H. suis-infected gastritis associated with gastric MALT lymphoma reported at our institution.Materials and Methods: Participants were selected from cases of gastric MALT lymphoma who underwent esophagogastroduodenoscopy at Hiroshima University Hospital, who were negative for the API2-MALT1 gene, and who received eradication therapy as a first-line treatment. We examined the endoscopic findings in nine cases from this group in which H. suis infection was confirmed by polymerase chain reaction.Results: Endoscopic findings, such as cracked mucosa, spotty redness, nodular gastritis-like appearance, and white marbled appearance, which have been reported as characteristics of NHPH gastritis, were observed in multiple cases. The most common endoscopic findings in this study were cracked mucosa (7/9 cases), followed by spotty redness (6/9 cases), nodular gastritis-like appearance (5/9 cases), and white marbled appearance (2/9 cases). Conclusions:Our study may serve as a reference for re-evaluation of the diagnostic criteria for H. suis infection and indications for eradication therapy, particularly for cases of H. pylori negative gastric MALT lymphoma, where endoscopic findings such as those seen in this study were observed in the background mucosa.

show abstract

Isolation and characterization of Helicobacter suis from human stomach

Cited by 22 publications

References 31 publications

Gastric Helicobacter species associated with dogs, cats and pigs: significance for public and animal health

Gastric Helicobacter species associated with dogs, cats and pigs: significance for public and animal health

Non-Helicobacter pylori Helicobacter (NHPH) positive gastric cancer

Investigation of endoscopic findings in nine cases of Helicobacter suis‐infected gastritis complicated by gastric mucosa‐associated lymphoid tissue lymphoma

Contact Info

Product

Resources

About