Testicular subcutaneous allografting followed by immunosuppressive treatment promotes maintenance of spermatogonial cells in rainbow trout (Oncorhynchus mykiss)

Yoshinaga, Túlio Teruo; Júnior, José Roberto Kfoury; Butzge, Arno Juliano; Olio, Rennan Lopes; Hernandez‐Blazquez, Francisco Javier; Carreira, Ana Cláudia Oliveira; Gomes, Cristina de Oliveira Massoco Salles; Bianchi, Pedro Kastein Faria da Cunha; Tabata, Yara Aiko

doi:10.1016/j.fsi.2021.03.002

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…In SSC transplantation studies in mice and rats, it has been found that the donors and recipients need to be closely related to avoid this potential problem; alternatively, immunodeficient animals can be used as recipients, or the recipients can be treated with drugs to suppress their immune responses (10, 11). Similar results have been reported in teleosts, in which allografted tissues are rejected within a few days or weeks by the immune system of the recipient (12-14). In teleosts, the immunorejection problem can be avoided by transplanting germ cells into newly hatched recipient larvae whose immune system are immature (15-18).…”

Section: Introductionsupporting

confidence: 87%

“…In some domesticated species, such as pigs, goats, and dogs, transplanted germ cells can be engrafted into recipient testes without the need to suppress the immune response (4,20,21). It has been reported that in fish, transplants of body tissue (scales) are rejected within a few days to about two weeks, while subcutaneous transplants of testicular tissue are accepted for six to nine weeks (12)(13)(14). While the mechanism of this immune privilege is not completely understood, it has nevertheless been of value for germ cell transplantation using adult fish as recipients (22).…”

Section: Introductionmentioning

confidence: 99%

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Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

Kayo

Kanda

Okubo

2022

Preprint

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Germ cell transplantation protocols generally require induced immunosuppression in the recipient to avoid rejection of the transplanted cells; cytotoxic drugs may also be used to deprive the recipient germ cells and create a germ cell niche that allows donor-derived germ cells to thrive. In the present study, we demonstrate that in medaka, allogeneic adult testicular tissue will engraft in adult recipients immediately after the rough castration of the recipients and without use of immunosuppressive drugs. The allografted testes are retained in the recipient body for at least three months and are able to produce viable gametes that yield offspring after natural mating. Some recipients showed a high frequency (over 60%) of offspring derived from spermatozoa produced by the transplanted germ cell tissues. Histological analyses showed that allografted testicular tissues included both germ cells and somatic cells that had established within an immunocompetent recipient testis. The relative simplicity of this new approach will benefit investigations of the basic processes of reproductive immunology and will improve the technique of gonadal tissue transplantation.

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Section: Introductionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

Kayo

Kanda

Okubo

2022

Preprint

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“…This study succeeded in developing a VASA160 marker that could differentiate F. merguiensis from P. vannamei and P. monodon . VASA is generally used as a marker to indicate the viability of germ cells within a target organism (Bar et al, 2016; Franěk et al, 2021; Li et al, 2017; Liu & Collodi, 2010; Pacchiarini et al, 2014; Ye et al, 2017; Yoshinaga et al, 2021). For example, VASA was used to follow pearl danio spermatogonia in zebrafish after xenogenic PGC transplantation; the PCR product of pearl danio VASA had a size of approximately 632 bp, whereas the PCR product of zebrafish VASA was approximately 207 bp (Saito et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular markers for differentiation of Fenneropenaeus merguiensis among Penaeus species

Chimnual

Saetan

Choomee

et al. 2022

Aquaculture Research

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Shrimp farming is a substantial income source in many countries. Farming is well established for some shrimp species, such as Penaeus vannamei (Pacific white shrimp), Macrobrachium rosenbergii (Freshwater shrimp) and Penaeus monodon (Black tiger shrimp).Despite Fenneropenaeus merguiensis (Banana shrimp) being wellknown to consumers, most of this shrimp production is from capture fisheries. This situation has led to overfishing of F. merguiensis, eventually damaging the 'species' genetic diversity and even threatening the extinction of this shrimp.Recently, transplantation technology has been applied successfully to several fish, including Nile tilapia (Oreochromis niloticus), rainbow trout (Oncorhynchus mykiss) and tiger puffer (Takifugu rubripes) (Farlora et al., 2014;Okutsu et al., 2007;Yoshikawa et al., 2018). This technique allows the transfer of the spermatogonia of a donor fish that exhibits desirable characteristics into a female recipient. This technology could be helpful in the genetic diversity conservation of F. merguiensis. However, applying this technology, that is, transferring spermatogonia from F. merguiensis to recipients of the same species or other shrimp species that could be good breeding partners, requires the availability of molecular markers that can be used to follow the genetic contributions of the donor in the recipient. VASA (Ddx4) is one of the markers used to detect germ cells in animals. The VASA gene is a member of the DEAD-box family (Asp-Glu-Ala-Asp) of ATP-dependent RNA helicases and is

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“…This finding is consistent with the general consensus that testes have immune privilege [ 15 ]. In a previous study on rainbow trout, testis allografted into subcutaneous tissue was retained for 6–9 weeks but rejected after 9 weeks [ 16 , 17 ]. In the present study, testicular allografts inserted into the abdomen of the recipient were retained for the full duration of our 13-week study (Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

2022

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Generally, successful testis transplantation has been considered to require immune suppression in the recipient to avoid rejection of the transplanted tissue. In the present study, we demonstrate in medaka that allogeneic adult testicular tissue will engraft in adult recipients immediately after partial castration without the use of immunosuppressive drugs. The allografted testes are retained in the recipient’s body for at least 3 months and are able to produce viable sperm that yield offspring after natural mating. Some recipients showed a high frequency (over 60%) of offspring derived from spermatozoa produced by the transplanted testicular tissue. Histological analyses showed that allografted testicular tissues included both germ cells and somatic cells that had become established within an immunocompetent recipient testis. The relative simplicity of this testis transplantation approach will benefit investigations of the basic processes of reproductive immunology and will improve the technique of gonadal tissue transplantation.

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Testicular subcutaneous allografting followed by immunosuppressive treatment promotes maintenance of spermatogonial cells in rainbow trout (Oncorhynchus mykiss)

Cited by 7 publications

References 31 publications

Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

Molecular markers for differentiation of Fenneropenaeus merguiensis among Penaeus species

Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

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