The molecular functions of Biodentine and mineral trioxide aggregate in lipopolysaccharide‐induced inflamed dental pulp cells

Wang, M. C.; Tu, Hsi Feng; Chang, Kuo‐Wei; Lin, Shun-Chieh; Yeh, Lo-Yao; Hung, Pei Shih

doi:10.1111/iej.13513

Cited by 9 publications

(9 citation statements)

References 43 publications

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“…Interestingly, the increase in cell viability was even higher in the LPS-activated hDPCs. As also reported herein, Biodentine promotes proliferation in both normal and LPS-induced DPCs (19). This might suggest that the activation with LPS, which simulated an inflammatory environment (pulpitis), may have a positive effect on the proliferative capacity of hDPCs.…”

Section: Discussionsupporting

confidence: 76%

“…In this study, no changes in TNF-α quantification in LPS-activated hDPCs were observed, which is in agreement with the literature (1). In addition, as observed here, CSBM neither induced nor aggravated LPS-induced inflammation (19).…”

Section: Discussionsupporting

confidence: 71%

“…Besides, there is strong evidence showing that CSBM increases the cell viability of hDPCs, it remains unclear how these materials induce healing and regeneration in inflamed human pulps (19). Thus, the results reported herein may shed a light on how these materials contribute to a better outcome in endodontic treatment.…”

Section: Discussionmentioning

confidence: 77%

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Exposure to lipopolysaccharide and calcium silicate-based materials affects the behavior of dental pulp cells

et al. 2022

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This study assessed the cell viability, cytokine production, and mineralization potential of human dental pulp cells (hDPCs) after exposure to lipopolysaccharide (LPS) and application of calcium silicate-based materials (CSBM). Characterization of the CSBM was performed by infrared spectroscopy (n = 3). Extracts of Bio-C Repair, Biodentine, Cimmo HD, and MTA Repair HP were prepared and diluted (1:1, 1:4, and 1:16). Culture of hDPCs was established and treated or not with 1 µg/mL of LPS from Escherichia coli for 7 days. MTT assay was used to assess cell viability at 24, 48, and 72 h (n = 6). Alkaline phosphatase (ALP) activity was assayed on day 7 (n = 4). Il-10 and TNF-α were quantified by ELISA at 24 h (n = 6). Data were analyzed by ANOVA and Tukey’s test (α = 0.05). Cell viability of LPS-activated hPDCs was higher than untreated control in 48 and 72 h (p < 0.05). Differences between non-treated and LPS-activated hPDCs were observed for Biodentine and Cimmo HP (p < 0.05). The CSBM influenced the cell viability (p < 0.05). ALP activity was higher in LPS-activated hDPCs (p < 0.05). No changes in the concentration of TNF-α were observed between groups (p > 0.05). The CSBM increased the Il-10 production (p < 0.05). LPS-activated hDPCs presented increased cell viability and ALP activity. The CSBM showed mild toxicity and was able to enhance the cell viability and mineralization potential of untreated and LPS-activated hDPCs. The CSBM also induced anti-inflammatory mechanisms without compromising pro-inflammatory ones.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 71%

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Exposure to lipopolysaccharide and calcium silicate-based materials affects the behavior of dental pulp cells

et al. 2022

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“…Calcium silicate, compared with MTA, improves cells’ viability, proliferation and reduces IL-1β levels [ 111 ]. Both Biodentine and MTA promote expression of DSPP, proliferation and adhesion of cells via AKT pathway [ 112 ].…”

Section: Resultsmentioning

confidence: 99%

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

et al. 2022

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Lipopolysaccharide (LPS) is widely used for induction of inflammation in various human tissues, including dental pulp. The purpose of this study was to summarize current medical literature focusing on (1) cell types used by researchers to simulate dental pulp inflammation, (2) LPS variants utilized in experimental settings and how these choices affect the findings. Our study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We searched for studies reporting outcomes of lipopolysaccharide application on dental pulp cells in vitro using electronic databases: MEDLINE, Web of Science and Scopus. Having gathered data from 115 papers, we aimed to present all known effects LPS has on different cell types present in dental pulp. We focused on specific receptors and particles that are involved in molecular pathways. Our review provides an essential foundation for further research using in vitro models of pulpitis.

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“…MTA has several desirable properties in terms of biocompatibility, bioactivity, hydrophilicity, radiopacity, sealing ability and low solubility [ 28 – 30 ]. In dentistry, two of the most important properties are its sealing ability and biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Survival analysis of pulpectomy in primary molars performed under dental general anaesthesia: a two-year retrospective study

Xie

Wang

et al. 2022

BMC Oral Health

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Background To retrospectively investigate the success rate of primary-molar pulpectomy performed under general anaesthesia and the potential risk factors that affect the 24-month success rate. Methods The case data and two-year follow-up records of children (aged 3–6 years) who received pulpectomy in primary molars performed under general anaesthesia were reviewed and assessed. Potential risk factors included age, gender, decayed-missing-filled teeth, endodontic diagnosis, tooth location, and postobturation sealing of the pulp chamber floor with MTA. With a two-year follow-up period, the outcomes of all the primary molars were classified into success and failure. Survival analysis was used to assess the outcomes. The Kaplan–Meier method was used to analyse the success rate. Univariate and multivariate Cox proportional hazards regression models were used to evaluate the potential risk factors associated with the overall survival of primary molars. Results A total of 410 teeth from 163 children (88 boys and 75 girls) were included in this study. The overall two-year success rate was 66.1% for all primary molars. The mean overall survival time for this study was 22.1 (95% CI, 21.73‒22.48) months. Multivariate Cox regression analysis demonstrated that endodontic diagnosis (irreversible pulpitis or periapical periodontitis), tooth location (maxillary or mandibular primary molar), and postobturation sealing of the pulp chamber floor (MTA or no-MTA) were significant risk factors for overall survival in this study (P < .05). The differences in success rates were not statistically significant in terms of age, gender, and decayed-missing-filled teeth (P > .05). Conclusions When compared to teeth diagnosed with irreversible pulpitis, those with periapical periodontitis failed more frequently. Postobturation sealing of the pulp chamber floor with MTA improved the success rate of pulpectomy in primary molars, especially when the inflammation did not spread to the periradicular area.

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The molecular functions of Biodentine and mineral trioxide aggregate in lipopolysaccharide‐induced inflamed dental pulp cells

Cited by 9 publications

References 43 publications

Exposure to lipopolysaccharide and calcium silicate-based materials affects the behavior of dental pulp cells

Exposure to lipopolysaccharide and calcium silicate-based materials affects the behavior of dental pulp cells

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

Survival analysis of pulpectomy in primary molars performed under dental general anaesthesia: a two-year retrospective study

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