Effect of exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) on mitochondrial DNA (mtDNA) copy number in rats

VanEtten, Samantha L.; Bonner, Matthew; Ren, Xuefeng; Birnbaum, Linda S.; Kostyniak, Paul J.; Wang, Jie; Olson, James R.

doi:10.1016/j.tox.2021.152744

Cited by 11 publications

(6 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The existence of fluctuation of copy number of mtDNA was previously reported in relation to injury and oxidative stress that contribute to the development of the toxicity of dioxin-like compounds [ 47 ] in acute myeloid leukemia (higher in aggressiveness stages) [ 48 ], hepatocellular [ 49 ], or tissue samples in breast cancer [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Arance

Ramírez

Rubio-Roldán

et al. 2021

Cancers

View full text Add to dashboard Cite

Here, the role of non-invasive biomarkers in liquid biopsy was evaluated, mainly in exosomes and mitochondrial DNA (mtDNA) as promising, novel, and stable biomarkers for renal cell carcinoma (RCC). A total of 140 fractions (named from B to F) obtained by ultracentrifugations of whole blood samples from 28 individuals (13 patients and 15 controls) were included. Nanoparticle Tracking Analysis (NTA) was conducted to characterized exosomal fraction. Subsequently, an analysis of digital PCR (dPCR) using the QuantStudio™ 3D Digital PCR platform was performed and the quantification of mtDNA copy number by QuantStudioTM 12K Flex Real-Time PCR System (qPCR) was developed. Moreover, Next Generation Sequencing (NGS) analyses were included using MiSeq system (Illumina, San Diego, CA, USA). An F fraction, which contains all exosome data and all mitochondrial markers, was identified in dPCR and qPCR with statistically significant power (adjusted p values ≤ 0.03) when comparing cases and controls. Moreover, present analysis in mtDNA showed a relevant significance in RCC aggressiveness. To sum up, this is the first time a relation between exosomal mtDNA markers and clinical management of RCC is analyzed. We suggest a promising strategy for future liquid biopsy RCC analysis, although more analysis should be performed prior to application in routine clinical practice.

show abstract

Section: Discussionmentioning

confidence: 99%

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Arance

Ramírez

Rubio-Roldán

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…ROS, or reactive oxygen species, react with cell membrane lipids to generate malondialdehyde (MDA) [42]. Oxidative stress contributes to the prolonged toxicity of TCDD [43]. The specific mechanism via which TCDD triggers oxidative stress is now unidentified.…”

Section: Discussionmentioning

confidence: 99%

Protection of Resveratrol Against Nephrotoxicity in Rats Produced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin

abdallah

2024

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

E CONDUCTED A STUDY to examine the impact of Resveratrol (RES) on the renal tissues of Wistar rats that were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and developed kidney damage. Applied on albino male rats (102), the age range ( 8-9 ) weeks and the weight range (80 -90) gms, (32) rats were used for acute stage of toxicity, while others (70 ) rats were involved as a chronic toxicity; the experiment design consists of 7 groups ; G1 (control -ve), G2 vehicle ( acetone + corn oil), G3 TCDD ( 4 µg/kg b. w.), G4 TCDD ( 2 µg/kg b. w.), G5 (RES), G6 (TCDD ( 4 µg/kg b. w.)+ RES),G (TCDD( 4 µg/kg b. w.) + RES). We found that malondialdehyde (MDA), urea and creatinine levels in the groups treated with TCDD showed significant increases compared to the other groups, despite a decrease in the levels of reduced glutathione (GSH) and catalase (CAT) in the TCDD groups. Comparing to the other groups, we observed a rise in GSH and CAT levels, as well as a decrease in MDA, urea, and creatinine levels in the RES treated group. The administration of RES improved the oxidative stress markers and histological alterations caused by TCDD.

show abstract

“…Moreover, lipid peroxidation and membrane structure damage of algal cells exposed to high concentrations of TCS may be one of the explanations for the hindrance of chlorophyll synthesis, which results in cell photosynthesis and growth inhibition. Dioxins, one of the transformation products of TCS, significantly enhances the oxidative stress response of organisms, thereby causing irreversible oxidative damage to organisms, indicating that dioxins produced by TCS transformation may also cause oxidative damage in E. gracilis [ 54 ]. In conclusion, the exposure of TCS and its transformation products resulted in oxidative stress, triggering cell structure damage and the impairment of cell function in E. gracilis , which can further threaten the growth and reproduction of the population.…”

Section: Discussionmentioning

confidence: 99%

Molecular Toxicity Mechanism Induced by the Antibacterial Agent Triclosan in Freshwater Euglena gracilis Based on the Transcriptome

Zhang

Yang

et al. 2023

Toxics

View full text Add to dashboard Cite

Triclosan (TCS), a commonly used antibacterial preservative, has been demonstrated to have high toxicological potential and adversely affects the water bodies. Since algae are one of the most significant primary producers on the planet, understanding the toxicological processes of TCS is critical for determining its risk in aquatic ecosystems and managing the water environment. The physiological and transcriptome changes in Euglena gracilis were studied in this study after 7 days of TCS treatment. A distinct inhibition ratio for the photosynthetic pigment content in E. gracilis was observed from 2.64% to 37.42% at 0.3–1.2 mg/L, with TCS inhibiting photosynthesis and growth of the algae by up to 38.62%. Superoxide dismutase and glutathione reductase significantly changed after exposure to TCS, compared to the control, indicating that the cellular antioxidant defense responses were induced. Based on transcriptomics, the differentially expressed genes were mainly enriched in biological processes involved in metabolism pathways and microbial metabolism in diverse environments. Integrating transcriptomics and biochemical indicators found that changed reactive oxygen species and antioxidant enzyme activities stimulating algal cell damage and the inhibition of metabolic pathways controlled by the down-regulation of differentially expressed genes were the main toxic mechanisms of TCS exposure to E. gracilis. These findings establish the groundwork for future research into the molecular toxicity to microalgae induced by aquatic pollutants, as well as provide fundamental data and recommendations for TCS ecological risk assessment.

show abstract

Effect of exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) on mitochondrial DNA (mtDNA) copy number in rats

Cited by 11 publications

References 64 publications

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Protection of Resveratrol Against Nephrotoxicity in Rats Produced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin

Molecular Toxicity Mechanism Induced by the Antibacterial Agent Triclosan in Freshwater Euglena gracilis Based on the Transcriptome

Contact Info

Product

Resources

About