LncRNA-SNHG6 promotes the progression of hepatocellular carcinoma by targeting miR-6509-5p and HIF1A

Abstract

Background Accumulating evidences have been reported that long noncoding RNAs play crucial roles in the progression of hepatocellular carcinoma (HCC). SnoRNA host gene 6 (SNHG6) is believed to be involved in several human cancers, but the specific molecular mechanism of SNHG6 in HCC is not well studied. Methods In this study, we experimentally down-regulated the SNHG6 in two hepatocellular carcinoma cell lines in vitro, and then measured the proliferation, migration and invasion abilities and the apoptotic levels. Also, we performed the xenograft assay to investigate the function of SNHG6 during the tumor growth in vivo. Results We found SNHG6 was highly expressed in HCC tissues. Next, using Hep3B and Huh7 cells, we confirmed knockdown of SNHG6 reduced the proliferation, migration and invasion abilities in vitro. Also, by bioinformatics analysis, further molecular and cellular experiments, we found miR-6509-5p bound to SNHG6 directly, and the expression level of HIF1A was regulated through SNHG6/miR-6509-5p axis. Finally, we found that down-regulation of SNHG6 dramatically reduced the tumor growth ability of Huh7 cells in vivo. Conclusions We concluded that SNHG6/miR-6509-5p/HIF1A axis functioned in the progression of hepatocellular carcinoma, and could be the promising therapeutic targets during the development of hepatocellular carcinoma drugs.

LncRNA SNHG6 promotes glycolysis reprogramming in hepatocellular carcinoma by stabilizing the BOP1 protein

“…LncRNA can mechanically act as a competing endogenous RNA (ceRNA) and bind with miRNAs to inhibit the effect of miRNA on their target mRNAs. SNHG6 promotes G1/S-phase transition in HCC by impairing miR-204-5p-mediated inhibition of E2F1 (Chen et al 2021 ) and promotes the progression of HCC by targeting miR-6509-5p and HIF1A (Fan et al 2021 ). In addition to combining with miRNAs, lncRNA has also been reported to be able to bind to proteins and affect their function.…”

The long non-coding RNA LIMT inhibits metastasis of hepatocellular carcinoma and is suppressed by EGF signaling

“…In the past 10 years, lncRNA has become a topic of intense interest in cancer research. LncRNAs play important roles in HCC by regulating cell proliferation, migration, and invasion [23][24][25]. The lncRNA LIMT has been identified as a novel and critical regulator in breast cancer [17].…”

The Hypoxia–Long Noncoding RNA Interaction in Solid Cancers

“…Numerous studies have shown that cancer progression is fostered by SNHG6 [ 170 , 171 , 172 , 173 ]. Moreover, SNHG6 can elevate the expression of HIF-1α by either sponging miRNAs or enhancing the translation of HIF-1α mRNA [ 150 , 151 , 152 ].…”

“…As a consequence, SNHG6 enhances migration and invasion abilities of hepatocellular carcinoma cells, along with an increase in HIF-1α expression. In xenografts, the growth of hepatocellular carcinoma is suppressed by the downregulation of SNHG6 [ 151 ] ( Figure 2 and Table 2 ).…”