Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans

Lilja, Stephanie; Stoll, Carina; Krammer, Ulrike; Hippe, Berit; Duszka, Kalina; Debebe, Tewodros; Höfinger, Ingrid; König, Jürgen; Pointner, Angelika; Haslberger, Alexander G.

doi:10.3390/ijms22052331

Cited by 29 publications

(13 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fecal samples were collected using INTEST.pro (Biomes Laboratory, Wildau, Germany) and then measured and analyzed by BIOMES laboratory (Wildau, Germany), using 16s rRNA NGS for fecal bacterial abundance, according to Lilja et al, 2021 [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

Zhang

Krämer

et al. 2022

IJMS

View full text Add to dashboard Cite

Roseburia intestinalis has received attention as a potential probiotic bacterium. Recent studies have demonstrated that changes in its intestinal abundance can cause various diseases, such as obesity, enteritis and atherosclerosis. Probiotic administration or fecal transplantation alter the structure of the intestinal flora, offering possibilities for the prevention and treatment of these diseases. However, current monitoring methods, such as 16S rRNA sequencing, are complex and costly and require specialized personnel to perform the tests, making it difficult to continuously monitor patients during treatment. Hence, the rapid and cost-effective quantification of intestinal bacteria has become an urgent problem to be solved. Aptamers are of emerging interest because their stability, low immunogenicity and ease of modification are attractive properties for a variety of applications. We report a FluCell-SELEX polyclonal aptamer library specific for R. intestinalis isolated after seven evolution rounds, that can bind and label this organism for fluorescence microscopy and binding assays. Moreover, R. intestinalis can be distinguished from other major intestinal bacteria in complex defined mixtures and in human stool samples. We believe that this preliminary evidence opens new avenues towards aptamer-based electronic biosensors as new powerful and inexpensive diagnostic tools for the relative quantitative monitoring of R. intestinalis in gut microbiomes.

show abstract

Section: Methodsmentioning

confidence: 99%

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

Zhang

Krämer

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…For mtDNA levels quantification, DNA was extracted from intestinal mucosa samples using QIAamp Fast DNA Tissue Kit (Qiagen, Germany). The quantification was performed as described previously [ 25 ]. Bacteria DNA levels were assessed by qRT-PCR following fecal DNA extraction (innuPREP Stool DNA Kit, Analytik Jena, Germany).…”

Section: Methodsmentioning

confidence: 99%

A Comparison of the Impact of Restrictive Diets on the Gastrointestinal Tract of Mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

The rate of gut inflammatory diseases is growing in modern society. Previously, we showed that caloric restriction (CR) shapes gut microbiota composition and diminishes the expression of inflammatory factors along the gastrointestinal (GI) tract. The current project aimed to assess whether prominent dietary restrictive approaches, including intermittent fasting (IF), fasting-mimicking diet (FMD), and ketogenic diet (KD) have a similar effect as CR. We sought to verify which of the restrictive dietary approaches is the most potent and if the molecular pathways responsible for the impact of the diets overlap. We characterized the impact of the diets in the context of several dietary restriction-related parameters, including immune status in the GI tract; microbiota and its metabolites; bile acids (BAs); gut morphology; as well as autophagy-, mitochondria-, and energy restriction-related parameters. The effects of the various diets are very similar, particularly between CR, IF, and FMD. The occurrence of a 50 kDa truncated form of occludin, the composition of the microbiota, and BAs distinguished KD from the other diets. Based on the results, we were able to provide a comprehensive picture of the impact of restrictive diets on the gut, indicating that restrictive protocols aimed at improving gut health may be interchangeable.

show abstract

“…Lilja and coworkers [146] investigated the gut microbiome and host gene expression before and after a Buchinger fast using 16S sequencing, revealing an overall change in microbiome structure yet with little that replicates in other available studies.…”

Section: Clinical Indications For Calorie Restrictionmentioning

confidence: 99%

Fasting intervention and its clinical effects on the human host and microbiome

Forslund

2022

J Intern Med

View full text Add to dashboard Cite

show abstract

Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans

Cited by 29 publications

References 49 publications

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

A Comparison of the Impact of Restrictive Diets on the Gastrointestinal Tract of Mice

Fasting intervention and its clinical effects on the human host and microbiome

Contact Info

Product

Resources

About