Black phosphorus incorporation modulates nanocomposite hydrogel properties and subsequent <scp>MC3T3</scp> cell attachment, proliferation, and differentiation

Xu, Haocheng; Liu, Xifeng; George, Matthew N.; Miller, Arthur K.; Park, Sungjo; Xu, Hao; Terzic, André; Lu, Lichun

doi:10.1002/jbm.a.37159

Cited by 9 publications

(6 citation statements)

References 51 publications

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“…As revealed consistently in the MTS test and live/dead stain, preferable cellular attachment and proliferation were displayed with the addition of BP into hydrogels, which is in line with the previous study. 9 The best cell behavior was detected in the OPF@BP-S group, indicating that the BP quantum dots may be most conducive to cell proliferation among BP nanosheets of different sizes.…”

Section: Discussionmentioning

confidence: 93%

“…Meanwhile, phosphate is a major component of cell structure, 8 such as cell membranes, nucleic acid, and adenosine triphosphate, and also helps to regulate bone mineralization and bone resorption, therefore making BP an attractive 2D material in bone tissue engineering. Our previous research and other published studies have found that followed by the exfoliation and integration, BP nanosheet‐based hydrogel platform could be used to accelerate bone regeneration via a sustained supply of phosphate 9,10 . However, BP nanosheets have been reported to be cytotoxic in certain conditions, 11 limiting the further application of BP nanosheets as biomaterials.…”

Section: Introductionmentioning

confidence: 99%

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Size‐dependent osteogenesis of black phosphorus in nanocomposite hydrogel scaffolds

Liu

Park

et al. 2022

J Biomedical Materials Res

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A promising new strategy emerged in bone tissue engineering is to incorporate black phosphorus (BP) into polymer scaffolds, fabricating nanocomposite hydrogel platforms with biocompatibility, degradation controllability, and osteogenic capacity. BP quantum dot is a new concept and stands out recently among the BP family due to its tiny structure and a series of excellent characteristics. In this study, BP was processed into nanosheets of three different sizes via different exfoliation strategies and then incorporated into cross-linkable oligo[poly(ethylene glycol) fumarate] (OPF) to produce nanocomposite hydrogels for bone regeneration. The three different BP nanosheets were designated as BP-L, BP-M, and BP-S, with a corresponding diameter of 242.3 ± 90.0, 107.1 ± 47.9, and 18.8 ± 4.6 nm. The degradation kinetics and osteogenic capacity of MC3T3 pre-osteoblasts in vitro were both dependent on the BP size. BP exhibited a controllable degradation rate, which increased with the decrease of the size of the nanosheets, coupled with the release of phosphate in vitro. The osteogenic capacity of the hydrogels was promoted with the addition of all BP nanosheets, compared with OPF hydrogel alone. The smallest BP quantum dots was shown to be optimal in enhancing MC3T3 cell behaviors, including spreading, distribution, proliferation, and differentiation on the OPF hydrogels. These results reinforced that the supplementation of BP quantum dots into OPF nanocomposite hydrogel scaffolds could potentially find application in the restoration of bone defects.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Size‐dependent osteogenesis of black phosphorus in nanocomposite hydrogel scaffolds

Liu

Park

et al. 2022

J Biomedical Materials Res

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“…BPs are emerging 2D layer-structured materials that possess unique physicochemical properties such as excellent photothermal conversion performance. In addition, previous studies have proved that BPs could be gradually oxidized to form a phosphate in a physiological environment, which drives biological mineralization procedures and promotes proliferation and osteogenic differentiation of BMSCs. ,− Based on the biological effects, BPs are usually applied to modify the surface of 3D-printed scaffolds or incorporated into hydrogels for bone defect repair. − In this study, BPs were introduced to the fracture site through a new way of metal implant coating, which could lay a material foundation for the dual functions of a photothermal antibiofilm and promotion of fracture healing elicited by BPs.…”

Section: Resultsmentioning

confidence: 99%

Black-Phosphorus-Nanosheet-Reinforced Coating of Implants for Sequential Biofilm Ablation and Bone Fracture Healing Acceleration

Yuan

Zhou

et al. 2022

ACS Appl. Mater. Interfaces

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Incurable implant-related infection may cause catastrophic consequences due to the existence of a biofilm that resists the infiltration of host immune cells and antibiotics. Innovative approaches inspired by nanomedicine, e.g., engineering innovative multifunctional bionic coating systems on the surface of implants, are becoming increasingly attractive. Herein, 2D black phosphorus nanosheets (BPs) were loaded onto a hydroxyapatite (HA)-coated metal implant to construct a BPs@HA composite coating. With its photothermal conversion effect and in situ biomineralization, the BPs@HA coating shows excellent performances in ablating the bacterial biofilm and accelerating fracture healing, which were verified through both in vitro and in vivo studies. Moreover, differentially expressed genes of bone formation and bone mesenchymal stem cells (BMSCs) regulated by the BPs@HA coating were identified using absolute quantitative transcriptome sequencing followed by the screening of gene differential expressions. A functional enrichment analysis reveals that the expression of core markers related to BMSC differentiation and bone formation could be effectively regulated by BPs through a metabolism-related pathway. This work not only illustrates the great potential in clinical application of the BPs@HA composite coating to eliminate bacteria and accelerate bone fracture healing but also contributes to an understanding of the underlying molecular mechanism of osteogenesis physiological function regulation based on an analysis of absolute quantitative transcriptome sequencing.

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“…In research, the addition of BPNs into a cross-linked OPF hydrogel improved the adhesion, distribution, proliferation, and differentiation of MC3T3-E1 cells. Optimal cell development was observed at BP doses up to 500 ppm [ 43 ]. In an additional research of 3D mesenchymal stem cell (MSC) preparations, Li et al [ 44 ] examined the osteogenic response of MSC spheroids by a novel combination of collagen and BP.…”

Section: Biomedical Application Of Black Phosphorus Hybrids Hydrogelmentioning

confidence: 99%

Research progress on black phosphorus hybrids hydrogel platforms for biomedical applications

Zhao

Jiang

et al. 2023

J Biol Eng

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Hydrogels, also known as three-dimensional, flexible, and polymer networks, are composed of natural and/or synthetic polymers with exceptional properties such as hydrophilicity, biocompatibility, biofunctionality, and elasticity. Researchers in biomedicine, biosensing, pharmaceuticals, energy and environment, agriculture, and cosmetics are interested in hydrogels. Hydrogels have limited adaptability for complicated biological information transfer in biomedical applications due to their lack of electrical conductivity and low mechanical strength, despite significant advances in the development and use of hydrogels. The nano-filler-hydrogel hybrid system based on supramolecular interaction between host and guest has emerged as one of the potential solutions to the aforementioned issues. Black phosphorus, as one of the representatives of novel two-dimensional materials, has gained a great deal of interest in recent years owing to its exceptional physical and chemical properties, among other nanoscale fillers. However, a few numbers of publications have elaborated on the scientific development of black phosphorus hybrid hydrogels extensively. In this review, this review thus summarized the benefits of black phosphorus hybrid hydrogels and highlighted the most recent biological uses of black phosphorus hybrid hydrogels. Finally, the difficulties and future possibilities of the development of black phosphorus hybrid hydrogels are reviewed in an effort to serve as a guide for the application and manufacture of black phosphorus -based hydrogels. Graphical Abstract Recent applications of black phosphorus hybrid hydrogels in biomedicine.

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Black phosphorus incorporation modulates nanocomposite hydrogel properties and subsequent MC3T3 cell attachment, proliferation, and differentiation

Cited by 9 publications

References 51 publications

Size‐dependent osteogenesis of black phosphorus in nanocomposite hydrogel scaffolds

Size‐dependent osteogenesis of black phosphorus in nanocomposite hydrogel scaffolds

Black-Phosphorus-Nanosheet-Reinforced Coating of Implants for Sequential Biofilm Ablation and Bone Fracture Healing Acceleration

Research progress on black phosphorus hybrids hydrogel platforms for biomedical applications

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