Complex microbial communities inhabiting natural Cordyceps militaris and the habitat soil and their predicted functions

Zhang, Xiaomei; Tang, De-Xiang; Li, Qingqing; Wang, Yuanbing; Xu, Zhihong; Li, Wenjun; Yu, Hong

doi:10.1007/s10482-021-01534-6

Cited by 12 publications

(7 citation statements)

References 51 publications

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“…At the phyla level, Ascomycota (99%) showed absolute abundance in C. militaris whereas Chytridiomycota (2.13%) and Unclassified_k__fungal (2.44%) were particularly observed in O. highlandensis , except Ascomycota (95.43%). At the genus level, Cordyceps exhibits absolute abundance (99.95%) in the fruiting body of C. militaris (Figure 3d), which is consistent with previous findings [33]. In O. highlandensis, Unclassified_k Hypocreales (83.68%) was the dominant genus (Figure 3d).…”

Section: Discussionsupporting

confidence: 90%

Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis

Zhang

Huang

et al. 2022

J Basic Microbiol

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Cordyceps sensu lato is a complex fungus‐larva symbiote, and its distribution is affected by the geography, climate, soil environment, and cohabitating microorganisms. However, despite the fact Cordyceps militaris and Ophiocordyceps highlandensis are different species, they coexist in the Pinus armandi forest of Dashao in Songming County, Yunnan, China. We explored the microbial compositions and soil metabolism inhabited by C. militaris and O. highlandensis using high‐throughput sequencing and nontargeted metabonomics. The results indicated that the bacterial and fungal compositions in the soil microhabitat communities of C. militaris, O. highlandensis, and null Cordyceps group were similar. However, the community compositions in the fruiting bodies of C. militaris and O. highlandensis were different. The dominant phylum Ascomycota and dominant genus Cordyceps were detected in the fruiting body of C. militaris. Except for Ascomycota, the dominant phylum Chytridiomycota and dominant genera Ophiocordyceps, Unclassified_k__Hypocreales, Circinotrichum, Cladosporium, and Unclassified_k__chytridiomycetesg were detected in the fruiting body of O. highlandensis. The plant probiotic bacteria Phyllobacterium was detected in the fruiting body of C. militaris. The growth‐promoting bacteria Pseudomonas was detected in the fruiting body of O. highlandensis. Soil metabolism analysis revealed that pathways associated with amino acid metabolism was significantly enriched in O. highlandensis. Correlation analysis of bacterial diversity and soil metabolites revealed that the relative abundances of bacterial operational taxonomic units and the relative contents of metabolites were consistent. Our results provide insights into the microbial diversity and soil metabolism of naturally coexisting C. militaris and O. highlandensis.

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Section: Discussionsupporting

confidence: 90%

Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis

Zhang

Huang

et al. 2022

J Basic Microbiol

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“…Some of these studies have involved Pleurotus spp. [14,15], Cordyceps militaris [16], and Ophiocordyceps highlandensis [17]. Nevertheless, little information on the microbial dynamics during substrate preparation or the cultivation of other mushrooms is known.…”

Section: Introductionmentioning

confidence: 99%

Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review

Suwannarach

Kumla

Zhao

et al. 2022

Biology

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Lignocellulosic materials commonly serve as base substrates for mushroom production. Cellulose, hemicellulose, and lignin are the major components of lignocellulose materials. The composition of these components depends upon the plant species. Currently, composted and non-composted lignocellulosic materials are used as substrates in mushroom cultivation depending on the mushroom species. Different substrate compositions can directly affect the quality and quantity of mushroom production yields. Consequently, the microbial dynamics and communities of the composting substrates can significantly affect mushroom production. Therefore, changes in both substrate composition and microbial diversity during the cultivation process can impact the production of high-quality substrates and result in a high degree of biological efficiency. A brief review of the current findings on substrate composition and microbial diversity for mushroom cultivation is provided in this paper. We also summarize the advantages and disadvantages of various methods of mushroom cultivation by analyzing the microbial diversity of the composting substrates during mushroom cultivation. The resulting information will serve as a useful guide for future researchers in their attempts to increase mushroom productivity through the selection of suitable substrate compositions and their relation to the microbial community.

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“…This study found that different cultivation environments had a greater impact on the composition and diversity of the bacterial community of C. chanhua ; the finding indicated that external microbial factors had a greater impact on the bacterial community composition of entomopathogenic fungi. The common genera of all C. chanhua samples were analyzed, and the common bacteria included 22 genera, such as Achromobacter , Serratia , Pseudomonas , Silesia , Enterococcus , and Bacillus , indicating that these bacterial groups played an important role in the growth and development of C. chanhua and these bacterial groups were also found in wild C. chanhua samples ( 29 ). Previous studies have reported that the competition between microorganisms can promote the production of new active substances or increase the content of existing metabolites ( 45 – 47 ).…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that bacteria can move with the help of fungal hyphae ( 51 , 52 ), so it is inferred that some bacteria in the mycoderm may come from the inner sclerotia. As the interface between C. chanhua and the environment, the mycoderm is a direct contact ( 29 ). We found that when the C. chanhua matured, the bacterial community on the mycelium was higher than the inner sclerotium.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have found that the relative abundance of other fungi in the sclerotia of C. chanhua was relatively low while the bacterial community was relatively rich ( 1 , 3 , 28 ). The mycoderm is the interface between C. chanhua and the environment, while the microbial community richness of entomopathogenic fungal mycoderm is significantly higher than that of inner sclerotia and bacteriosphere soil ( 29 , 30 ). Therefore, exploring the biofiltration effect of C. chanhua membrane on a bacterial community is a specific priority in this study.…”

Section: Introductionmentioning

confidence: 99%

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Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

Hu,

Zhou,

Mou

et al. 2024

Microbiol Spectr

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Cordyceps chanhua is a kind of entomopathogenic fungus that has a long medicinal history in traditional Chinese medicine. In this study, we aimed to explore the role of C. chanhua mycoderm. Our results showed that C. chanhua mycoderm has a certain ability to transfer nitrogen, which can transfer nitrogen from the insect body to the external environment and transfer nitrogen from the environment to C. chanhua . By studying the filtration of bacterial mycoderm, it was found that the mycoderm of C. chanhua was able to filter out most of the bacteria in the environment. Using the high-throughput sequencing methods, we found that the abundance of bacterial community first increased and then decreased during the growth and development of C. chanhua . The bacterial richness and diversity of mycoderm of C. chanhua cultivated in sterile glass bottles were significantly lower than those cultivated in soil mulching. In addition, this study also found that the mycoderm of C. chanhua formed under both soil mulching cultivation and sterile glass bottle cultivation was hydrophobic. The mycoderm of C. chanhua , which occurred under sterile glass bottle cultivation, was closely and orderly arranged, while the mycoderm of C. chanhua cultivated in soil was quite the opposite. The density and thickness of the C. chanhua membrane in aseptic glass bottle culture were higher than those in soil-covered culture, but the dry/fresh weight was lower than those in soil-covered culture. IMPORTANCE During the natural growth of Cordyceps chanhua , it will form a mycoderm structure specialized from hyphae. We found that the bacterial membrane of C. chanhua not only filters environmental bacteria but also absorbs and transports nitrogen elements inside and outside the body of C. chanhua . These findings are of great significance for understanding the stable mechanism of the internal microbial community maintained by C. chanhua and how C. chanhua maintains its own nutritional balance. In addition, this study also enriched our understanding of the differences in bacterial community composition and related bacterial community functions of C. chanhua at different growth stages, which is of great value for understanding the environmental adaptation mechanism, the element distribution network, and the changing process of symbiotic microbial system after Cordyceps fungi infected the host. At the same time, it can also provide a theoretical basis for some important ecological imitation cultivation technology of Cordyceps fungi.

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Complex microbial communities inhabiting natural Cordyceps militaris and the habitat soil and their predicted functions

Cited by 12 publications

References 51 publications

Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis

Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis

Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review

Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

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