Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Abstract

Micro RNA (miR) are recognized for their important roles in biological processes, particularly in regulatory componentization. Among the miR, miR-150 has been the focus of intense scrutiny, mostly due to its role in malignant tumors. A comparison between steer and bull adipose tissues identified bta-miR-150 as one of the nine downregulated miRNAs, although its function remains unknown (GEO:GSE75063). The present study aimed to further characterize the role of bta-miR-150 in cattle. bta-miR-150 has a negative regulatory effect on the differentiation of bovine adipocytes and promotes proliferation. Overexpression of bta-miR-150 can promote mRNA and protein expression of the marker genes CDK1, CDK2, and PCNA, increase the number of EdU-stained cells, promote adipocyte proliferation, inhibit adipocyte differentiation, and reduce lipid droplet formation. Results of RNA-seq and WGCNA analyses showed that the mammalian target of the rapamycin signaling pathway, which plays a major regulatory role, is dysregulated by the overexpression and inhibition of miR-150. We found that the target gene of bta-miR-150 is AKT1 and that bta-miR-150 affects AKT1 phosphorylation levels. These results showed that bta-miR-150 plays a role in adipogenic differentiation and might therefore have applications in the beef industry.

A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle

“…This means that many previous studies focusing on humans and mice have lost their original reference value in the field of livestock production. Additionally, most studies on animal adipogenesis have focused on subcutaneous and perirenal fat [13,[15][16][17][18][19][20][21], with fewer studies on intramuscular adipose tissue. The origin and development of IMF are different from that of other adipose tissues, which makes it impossible to fully replicate the studies of other adipose tissues in IMF tissue as well [6][7][8].…”

“…miR-NAs are strongly and functionally conserved among species and were the first noncoding RNAs identified and characterized [14]. Our previous studies have shown that bta-miR-150 regulates the mTOR pathway by targeting the 3'UTR of AKT1, thereby affecting the proliferation and differentiation of bovine preadipocytes [15]. bta-miR-376a inhibited bovine adipocyte differentiation through the KLF15/PPARG axis [16].…”

Identification of the hub genes related to adipose tissue metabolism of bovine

“…The results were expressed as mean ± standard error of the mean (SEM). Statistical analyzes were performed with SPSS 25 (37) and Graphpad Prism 9 (38). Differences between groups were * p < 0.05, significant; * * p < 0.01, moderately significant; * * * p < 0.001, highly significant; and * * * * p < 0.0001, extremely significant.…”

“…It should be noted that three biological replicates and technical replicates were performed for all experiments. SPSS 25 ( 37 ) and Graphpad Prism 9 ( 38 ) softwares were used for difference significance analysis and mapping, respectively.…”

“…The results were expressed as mean ± standard error of the mean (SEM). Statistical analyzes were performed with SPSS 25 ( 37 ) and Graphpad Prism 9 ( 38 ). Differences between groups were calculated by Analysis of Variance (ANOVA) methods and significance was indicated by lowercase letters or asterisks.…”