Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation

Abstract

Relationship between lymphocyte function and cardiorespiratory fitness (CRF) is well documented at rest; however, upon mitogen stimulation the proliferation and cytokine production alters but are incipient the lymphocyte responses front mitogens according to the CRF. So, the purpose of the present study was to analyze the lymphocyte function according to the physical fitness status of healthy young men. The study is divided in two experiments being the first analyzing the lymphocyte phenotypes profile and the inflammatory responses, according to CRF, in lymphocyte cell culture treated for 48 hours with Concanavalin A (ConA). And the second experiment analyzed the proliferation, reactive oxygen species production, viability, and mitochondrial polarization state in lymphocytes treated with ConA in different concentrations, considering the CRF levels. The results showed a difference in the percentage of total lymphocytes expression between groups (p=0.011) observing a lower lymphocytes T expression in the group with High when compared with Moderate group. When treated with ConA, the lymphocytes of the Low group released higher TNF-α concentration (p=0.032), reflecting an elevated TNF-α/IL-10 ratio (p=0.055), parallel with lower IL-6 production (p=0.027), mainly when compared with Moderate group. In addition, there is a positive relationship between and IL-6 production (r=0.507; p=0.016) whereas the percentage of total lymphocytes (LyT%) shows a negative association tendency with (r=-0.497; p=0.060). Also, individuals with lower showed reduced absolute and relative ROS production, lower cell proliferation, and higher mitochondrial membrane depolarization. In conclusion, cardiorespiratory fitness degree exerts a strong impact on lymphocyte function after mitogen stimulation.

Influence of age and fitness level on immune responses of T and NK cells in healthy physically active subjects after strenuous aerobic exercise: a cross-sectional study

“…However, according to the study conducted by Dorneles et al. ( 37 ), fitness status seems to directly affect T-lymphocyte function, i.e., those individuals with lower fitness have higher circulating T-lymphocytes and a consequent higher pro-inflammatory state. Furthermore, in another study conducted by Dorneles et al.…”

“…However, according to the study conducted by Dorneles et al (37), fitness status seems to directly affect T-lymphocyte function, i.e., those individuals with lower fitness have higher circulating Tlymphocytes and a consequent higher pro-inflammatory state. Furthermore, in another study conducted by Dorneles et al (38), it was identified that after three sessions of high-intensity interval training (HIIT) with 48h of recovery between sessions, low fitness exerted a negative impact on several immune system markers.…”