Temperature-robust and ratiometric G-quadruplex proximate DNAzyme assay for robustly monitoring of uranium pollution and its microbial biosorbents screening

Yang, Yong; Yang, Hao; Wan, Yi; Zhou, Wenhu; Deng, Sha; He, Yao; He, Guiping; Xie, Xiang; Deng, Ruijie

doi:10.1016/j.jhazmat.2021.125383

Cited by 21 publications

(7 citation statements)

References 43 publications

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“…The key mechanism of the cDNAzyme assay lies in the dual cleavage of DNAzyme cutting and CRISPR reporting, which both are highly affected by the hybridization of Sub-Dz . Twelve Dz s with varying arm lengths were designed (Figure S4, in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice

Yang

Xue

et al. 2022

Anal. Chem.

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Lead contamination in the environment tends to enter the food chain and further into the human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme tandem assay (termed cDNAzyme) using CRISPR/Cas III-A Csm6 and GR-5 DNAzyme, enabling one-pot and sensitive detection of lead contamination. We found that Pb2+-activated GR-5 DNAzyme produced cleaved substrates that can serve as the activator of Csm6, and the Csm6-DNAzyme tandem improved the sensitivity for detecting Pb2+ by 6.1 times compared to the original GR-5 DNAzyme. Due to the high specificity of DNAzyme, the cDNAzyme assay can discriminate Pb2+ from other bivalent and trivalent interfering ions and allowed precise detection of Pb2+ in water and food samples. Particularly, the assay can achieve one-step, mix-and-read detection of Pb2+ at room temperature. We used the cDNAzyme assay to investigate the accumulation of lead in mice, and found that lead accumulated at higher levels in the colon and kidney compared to the liver, and most of the lead was excreted. The cDNAzyme assay is promising to serve as analytical tools for lead-associated environmental and biosafety issues.

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Section: Resultsmentioning

confidence: 99%

Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice

Yang

Xue

et al. 2022

Anal. Chem.

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“…Reprinted with permission ( Zhu et al, 2019 ), Copyright 2019 Elsevier B.V. (B) Ratiometric fluorescent biosensor for uranyl detection. Reprinted with permission ( Yang et al, 2021 ). Copyright 2021 Elsevier B.V. (C) Electrochemical biosensor for uranyl detection based on DNAzyme and CHA.…”

Section: Applications Of Different Types Of Dnazyme-based Sensorsmentioning

confidence: 99%

“…Increased fluorescent signals enabled the quantitative detection of uranyl ions. Yang et al reported a ratiometric fluorescent DNAzyme sensor for UO 2 2+ monitoring ( Yang et al, 2021 ). The fluorescent biosensor contained DNAzyme probes (39E-DNA and 39S-DNA) and the split G-quadruplex probes (39F-R and 39F-L) as shown in Figure 1B .…”

Section: Applications Of Different Types Of Dnazyme-based Sensorsmentioning

confidence: 99%

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Bai

Chai

et al. 2022

Front. Chem.

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Nuclear facilities are widely used in fields such as national defense, industry, scientific research, and medicine, which play a huge role in military and civilian use. However, in the process of widespread application of nuclear technology, uranium and its compounds with high carcinogenic and biologically toxic cause a lot of environmental problems, such as pollutions of water, atmosphere, soil, or ecosystem. Bioensors with sensitivity and specificity for the detection of uranium are highly demand. Nucleic acid enzymes (DNAzyme) with merits of high sensitivity and selectivity for targets as excellent molecular recognition elements are commonly used for uranium sensor development. In this perspective review, we summarize DNAzyme-based biosensors for the quantitative detection of uranyl ions by integrating with diverse signal outputting strategies, such as fluorescent, colorimetry, surface-enhanced Raman scattering, and electrochemistry. Different design methods, limit of detection, and practical applications are fully discussed. Finally, the challenges, potential solutions, and future prospects of such DNAzyme-based sensors are also presented.

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“…2 Uranium(VI) is present in the form of uranyl cations (UO 2 2+ ) in water solutions, 3 having strong mobility and easy access to the food chain, which will have a serious impact on human health. [4][5][6] The demand for nuclear resources is increasing, but the reserve of uranium on land is limited. 7 Therefore, how to extract U(VI) from natural seawater or contaminated seawater becomes an urgent environmental and energy issue.…”

Section: Introductionmentioning

confidence: 99%

Highly selective and ultrafast uptake of uranium from seawater by layered double hydroxide co-intercalated with acetamidoxime and carboxylic anions

Wang

Yang

et al. 2022

J. Mater. Chem. A

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Temperature-robust and ratiometric G-quadruplex proximate DNAzyme assay for robustly monitoring of uranium pollution and its microbial biosorbents screening

Cited by 21 publications

References 43 publications

Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice

Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Highly selective and ultrafast uptake of uranium from seawater by layered double hydroxide co-intercalated with acetamidoxime and carboxylic anions

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