Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale

Burdge, Justin; Fried, Nathan T.; Abdus-Saboor, Ishmail

doi:10.1016/j.xpro.2021.100322

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…These approaches include increasing camera resolution of videographic techniques to reduce experimenter interference and improve ease of manual scoring. 66–68 These new technologies allow more experimenters to identify more precise observations in an automated, objective manner. Moreover, these technologies have the potential to simplify data comparison across studies and laboratories.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, these technologies have the potential to simplify data comparison across studies and laboratories. 66 For instance, results from freely moving animals captured by machine-detected, automatic systems can be reported as ethograms, depositing records of animal behaviors into databases. 67 Recently, Bohnslav and colleagues developed DeepEthogram, 67 a Python program capable of learning to automatically detect and label distinct behaviors, allowing for minimal user input.…”

Section: Discussionmentioning

confidence: 99%

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Advanced Dynamic Weight Bearing as an Observer-independent Measure of Hyperacute Hypersensitivity in Mice

Dent,

Segal,

Brécier

et al. 2023

Canadian Journal of Pain

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Background Standard methods assessing pain in rodents are often observer dependent, potentially resulting in biased outcomes. Advanced dynamic weight bearing (ADWB) offers an observer-independent approach that can provide objective, reliable data in preclinical pain research. Aims The aim of this study was to characterize the use of ADWB in assessing murine responses to allyl isothiocyanate (AITC)-induced hyperacute hypersensitivity and identify best practices for use of the device. Methods Male C57BL/6J mice received intraplantar injections of saline or 0.1% AITC solution and were assessed using the ADWB system; simultaneous observer-dependent durations of paw licking and biting were measured. ADWB data were analyzed using the proprietary software from Bioseb and correlated to observer-dependent results, with parameters assessed to optimize data collected. Results ADWB detected pain-directed changes in weight and surface area distribution in AITC-treated mice, with paw weight and surface area placement correlating to paw licking and biting. Optimization of adjustable threshold parameters allowed for reduced coefficients of variability and increased duration of validated data. Conclusions The ADWB assay provides an efficient and unbiased measure of chemical-induced hyperacute hypersensitivity in mice. ADWB detection parameters influence amount of validated data and variability, a consideration for data analysis in future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Advanced Dynamic Weight Bearing as an Observer-independent Measure of Hyperacute Hypersensitivity in Mice

Dent,

Segal,

Brécier

et al. 2023

Canadian Journal of Pain

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“…Additionally, injured mice displayed guard postures after withdrawal, characterized by a delayed return of the paw to the mesh surface (Fig. 1E) 27 . These findings suggest that mice developed increased mechanical hypersensitivity in their hindpaws following complete CST injury and had lower tolerance for full weight support on the grid texture.…”

Section: Chronic Cst Lesion Induces An Allodynia-like Phenotypementioning

confidence: 99%

Neuropathic pain in a chronic CNS injury model is mediated by CST-targeted spinal interneurons

Guan,

Zhu,

Zhong

et al. 2024

Preprint

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Chronic neuropathic pain is a persistent and debilitating outcome of traumatic central nervous system injury, affecting up to 80% of individuals with chronic injury. Post-injury pain is refractory to clinical treatments due to the limited understanding of the brain-spinal cord circuits that underlie pain signal processing. The corticospinal tract (CST) plays critical roles in sensory modulation during skilled movements and tactile sensation; however, a direct role for the CST in injury-associated neuropathic pain is unclear. Here we show that complete, selective CST transection at the medullary pyramids leads to hyperexcitability within lumbar deep dorsal horn and hindlimb allodynia in chronically injured adult mice. Chemogenetic regulation of CST-targeted lumbar spinal interneurons demonstrates that dysregulation of activity in this circuit underlies the development of tactile allodynia in chronic injury. Our findings shed light on an unrecognized circuit mechanism implicated in CNS injury-induced neuropathic pain and provide a novel target for therapeutic intervention.

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“…To increase the dynamic range of sensory-evoked behavior assays we used high-speed videography to break down paw withdrawal to sensory stimuli into sub-second movements within groups of recently established short-latency reflexive vs. long-latency affective behavioral families 15,16 (Fig.2a-c). Mechanical hypersensitivity is a common complaint of inflammatory pain, presenting itself as either allodynia, when innocuous sensations become painful 17–19 , or as hyperalgesia, when there is an increased sensitivity to noxious sensations 20,21 .…”

Section: Mainmentioning

confidence: 99%

Mapping the signatures of inflammatory pain and its relief

Bohic

et al. 2021

Preprint

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Inflammatory pain represents a complex state involving sensitization of peripheral and central neuronal signaling. Resolving this high-dimensional interplay at the cellular and behavioral level is key to effective therapeutic development. Here, using the carrageenan model of local inflammation of the hind paw, we determine how carrageenan alters both the physiological state of sensory neurons and behaviors at rapid and continuous timescales. We identify higher excitability of sensory neurons innervating the site of inflammation by profiling their physiological state at different time points. To identify millisecond-resolved sensory-reflexive signatures evoked by inflammatory pain, we used a combination of supervised and unsupervised algorithms, and uncovered abnormal paw placement as a defining behavioral feature. For long-term detection and characterization of spontaneous behavioral signatures representative of affective-motivational pain states, we use computer vision coupled to unsupervised machine learning in an open arena. Using the non-steroidal anti-inflammatory drug meloxicam to characterize analgesic states during rapid and ongoing timescales, we identify a return to pre-injury states of some sensory-reflexive behaviors, but by and large, many spontaneous, affective-motivational pain behaviors remain unaffected. Taken together, this comprehensive exploration across cellular and behavioral dimensions reveals peripheral versus centrally mediated pain signatures that define the inflamed state, providing a framework for scaling the pain experience at unprecedented resolution.

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Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale

Cited by 5 publications

References 3 publications

Advanced Dynamic Weight Bearing as an Observer-independent Measure of Hyperacute Hypersensitivity in Mice

Advanced Dynamic Weight Bearing as an Observer-independent Measure of Hyperacute Hypersensitivity in Mice

Neuropathic pain in a chronic CNS injury model is mediated by CST-targeted spinal interneurons

Mapping the signatures of inflammatory pain and its relief

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