Cholesterol Effect on Membrane Properties of Cationic Ion Pair Amphiphile Vesicles at Different Temperatures

Abstract

This work is focused on the study of the effect of cholesterol on the properties of vesicular membranes of ionic amphiphilic pairs at different temperatures. The hexadecyltrimethylammonium-dodecyl sulfate ionic amphiphilic pair system with the addition of 10 mol % dioctadecyldimethylammonium chloride was chosen for a detailed study of vesicle properties. A large range of cholesterol concentrations (0–73 mol %) in the temperature range 10–80 °C was studied. Under these conditions, the size distribution, the membrane fluidity, and the surface layer were monitored together with the change in the mobility of water in the surface layer. Obtained quantities were correlated with each other and combined into appropriate graphs. It was found that in stable systems that meet the condition of unimodal size distribution with a PDI value lower than 0.3, temperature has virtually no effect on the size of vesicular systems. On the contrary, when studying the hydration and fluidity of the membrane, significant changes in these parameters were found, which, however, do not affect the short-term stability of these vesicular systems. The presented results thus indicate the possibility of adjusting the composition of the vesicular system in terms of fluidity and membrane hydration while maintaining short-term stability and size distribution.

Hypo-Osmotic Stress and Pore-Forming Toxins Adjust the Lipid Order in Sheep Red Blood Cell Membranes

“…The sigmoidal plots have been fitted in Origin 8.5.2 with the four-parameter Boltzmann function [26,28]:…”

“…where max and min are the y coordinates of the plateaus, x 0 is the x-coordinate of the inflex points, and dx is the slope of the corresponding dependence [28].…”

“…of cholesterol, we considered the changes in lipid ordering induced by temperature variations in membranes undergoing phase transitions [26,28,31,32,38,47]. Artificial membrane systems comprising few lipid components with no cholesterol exist in a highly ordered gel phase at low temperature (i.e., under the melting temperature).…”

Medical gloves modified by a one-minute spraying process with blood-repellent, antibacterial and wound-healing abilities

“…33 In addition, DDAC could also interfere with the synthesis of nucleic acids and proteins. 34 In short, DDAC had the advantages of good stability, low toxicity, insipidity, non-irritation and reliable sterilization. The antibacterial activity of MS was also verified by the live/ dead viability assay using PI and N01 staining.…”

Cholesterols Induced Distinctive Entry of the Graphene Nanosheet into the Cell Membrane

“…Cholesterols, which are multifunctional lipids unique to the eukaryotic membrane, are distributed in various tissues with amounts varying from approximately 20 to 50% The rigid steroid ring structure of cholesterol restricts the motion of other membrane lipid tails, which thereby modulates biophysical properties of mammalian plasma membranes, such as membrane thickness, fluidity, , and flexibility. , Despite the role of cholesterols in biosystems having been reported by a large amount of literature studies, , much remains unknown about the exact roles of cholesterols and their molecular interactions on the membrane function. , Based on their effect on the membrane permeability and molecular transport ability, Canepa et al reported that the passive uptake of amphiphilic nanoparticles into fluid lipid membranes can be significantly hindered by cholesterols . Lorents et al.…”