Evaluation of the antiseptic and wound healing potential of polyhexamethylene guanidine hydrochloride as well as its toxic effects

Dias, Fernanda Gosuen Gonçalves; Pereira, Lucas de Freitas; Parreira, Renato L. T.; Veneziani, Rodrigo Cássio Sola; Bianchi, Thamires Chiquini; Fontes, Vitória Ferreira Nogueira de Paula; Galvani, Marina de Carlos; Cerce, Danielle Dal Picolo; Martins, Carlos Henrique Gomes; Rinaldi-Neto, Francisco; Ferreira, Natália Helen; Silva, Lucas Henrique Domingos da; Oliveira, Lucas Teixeira Souza de; Esperandim, Tábata Rodrigues; Sousa, Francielle Aparecida de; Ambrósio, Sérgio Ricardo; Tavares, Denise Crispim

doi:10.1016/j.ejps.2021.105739

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…The PHMBH has not worse stimulating effect on the proliferation activity and monolayer growth than PHMGH in similar concentrations. Both PHMGH and PHMBH have been suggested as possible candidates to be included in growth stimulators or wound-healing medicines (Kamenieva TM et al, 2020;Dias FGG et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…PHMG is also known for its anti-infl ammatory and wound-healing properties and to act as an anti-oxidant, so it was found to be useful in the treatment of chronic wounds and thermal burns (Lebedeva et al, 2017;Han et al, 2019;Kamenieva TM et al, 2020;Dias FGG et al, 2021), PHMBH is also known (Gray et al, 2010;Paydar et al, 2017;Oluwole et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Lysytsya¹,

Kryvoshyya²,

Kvartenko³

et al. 2022

Agric. sci. pract.

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Aim. To investigate both toxic (hemolytic), and stimulating effects of two polymeric derivatives of guani- dine, in particular, polyhexamethylene guanidine (PHMG) and polyhexamethylene biguanidine (PHMB) both in the hydrochloride form, on eukaryotic cells depending on the concentration of the preparation; to study the possibility of using wound-healing and stimulating properties of these preparations in veterinary medicine. Methods. The hemolytic activity (toxicity) of PHMGH and PHMBH preparations in the concentration of 0.1% towards cattle and pig erythrocytes was determined by titration. Primary cell cultures of fetal kidney cells of calves and piglets were used to determine the influence of PHMGH and PHMBH both alone and in combination with the following biologically active substances: essential oils of Pinus sylvestris, Eucalyptus globulus, Citrus sinensis, Monarda didyma, ZnO nanoparticles (size c. 25 nm), and electrochemically acti- vated water – anolyte (Eh = –800 mV, pH 6.5–7.0). The concentration of the cells in the nutrient medium was determined via photocolorimetry. Results. It was found that depending on the concentration, PHMGH, and PHMBH preparations can cause the lysis of erythrocytes, and stimulate cell proliferative activity, including the formation of a monolayer of kidney cells of calves and piglets They cause hemolysis of cattle erythro- cytes in the concentrations commonly used for disinfection, i.e., about 0.1 %, in the average titers of 1 : 7 for PHMGH and 1 : 2.5 for PHMBH. Therefore, PHMBH shows greater hemolytic (biocidal) activity for cattle erythrocytes than PHMGH (in ≈2.8x). The high molecular weight fraction of PHMBH (M2 ≈ 2,000–7,000 Da) demonstrated a lower (in ≈2.4x) hemolytic activity than the low molecular weight basic fraction (M1 ≈ 500–2,000 Da). The experiments on the kidney cell cultures of pigs and cattle have shown that at non-toxic concentrations (10–5 %) PHMBH can effectively stimulate (from 27 to 65 % increase) the proliferative activ- ity of eukaryotic cells and accelerate the formation of a monolayer of cells. The combinations of PHMGH with some essential oils of medicinal plants also show a good effect (from 52 to 95 % increase), and PHMBH shows a good effect with oil of pine for pig kidney cells (20 % increase) and oil of horsemint for cattle kidney cells (67 % increase). Conclusions. PHMGH and PHMBH can possibly be used in agricultural production not only as disinfectants or antiseptics, but also in wound healing. Although their toxicity is also significant to eukaryotic cells, yet they can possibly be used in veterinary medicine in low concentrations (0.005–0.5 %) for the treatment of wounds of various origin, including burns, in the composition of ointments, gels, bandages, or plasters, which we have presently in investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Lysytsya¹,

Kryvoshyya²,

Kvartenko³

et al. 2022

Agric. sci. pract.

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“…The acute toxicity studies on rats showed that the median lethal dose (LD 50 ) for PHMG-Cl is significantly higher (600 mg/kg) [45] than for PHMB-Cl (25.6 mg/kg) [46]. In other study, results of acute oral toxicity showed that PHMG-Cl at 5% presents a lethal dose 50% greater than the 2000 mg/kg [47]. From a practical point of view, PHMG-Cl seems very promising antimicrobial additive for thermoplastic PUs.…”

Section: Introductionmentioning

confidence: 88%

New promising antimicrobial material based on thermoplastic polyurethane modified with polymeric biocide polyhexamethylene guanidine hydrochloride

Rogalsky

Bardeau

Lyoshina

et al. 2021

Materials Chemistry and Physics

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“…Polyhexamethylene guanidine is a bactericidal agent that has been used in many applications such as antiseptics, 150 water treatment, 151 the food industry 152 and personal hygiene applications. 153 Zhuo et al 154 synthesized three oligoguanidine hydrochloride polymers bearing various alkyl chains e.g.…”

Section: Polymer Solutions As Antimicrobial Agentsmentioning

confidence: 99%

Polymers showing intrinsic antimicrobial activity

Haktanıyan

Bradley

2022

Chem. Soc. Rev.

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Evaluation of the antiseptic and wound healing potential of polyhexamethylene guanidine hydrochloride as well as its toxic effects

Cited by 11 publications

References 33 publications

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

New promising antimicrobial material based on thermoplastic polyurethane modified with polymeric biocide polyhexamethylene guanidine hydrochloride

Polymers showing intrinsic antimicrobial activity

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