This study analysed the effect of pastes formulated with calcium hydroxide P.A. and different vehicles (saline solution -paste A and Copaifera langsdorffii Desfon oil -paste B) on oral microorganisms and dentin bridge formation in dogs. The antimicrobial action of the pastes and their components was analysed by the minimum inhibitory concentration in agar gel RESUMO Foi avaliada a ação de pastas formuladas com hidróxido de cálcio P.A. e diferentes veículos (solução fisiológica -pasta A e óleo de Copaifera langsdorffii Desfon -pasta B) sobre microrganismos bucais e formação de ponte dentinária em cães. A ação antimicrobiana das pastas e de seus componentes individuais foi avaliada pela técnica da concentração inibitória mínima pela diluição em ágar. Os materiais foram diluídos e avaliados contra quinze cepas padrão de microrganismos relacionados a doenças endodônticas. Os microrganismos foram cultivados e, após a incubação, os dados foram analisados (Anova OneWay, Tukey, P≤0,05). Para a avaliação da formação de ponte dentinária, quatro incisivos superiores de dez animais foram tratados, sendo dois capeados com pasta A (GA) e dois com a pasta B (GB). Após 90 dias, os dentes foram extraídos para

Amblyomma incisum Neumann is a major tick species in the Atlantic Forest of Brazil. Tapir is the main host for adult ticks and a high aggressiveness of nymphs to humans has been reported. In this work data on the biology and life cycle of this tick species is presented for the first time. It was shown that horse is a suitable host for A. incisum adults and rabbit for larvae and nymphs. It was also shown that A. incisum is a big tick species (mean engorged female weight of 1.96 g) with a long life cycle which lasts 262.3 days when maintained at 27 degrees C and 85% RH. These laboratory conditions were, however, inappropriate and egg hatching rate (1.2%) was very low. Nevertheless egg hatching of ticks in a forest patch increased considerably (72.2%) indicating that this A. incisum population is highly dependent on a forest-like environment.

PURPOSE:To describe video-assisted ovariohysterectomy (OHE) with two portals access in adult intact queens. METHODS:Fifty-two females cats were used. A 4 mm cannula was positioned in the abdomen through an incision close to the umbilicus (first portal), and a pneumoperitoneum was established. A second portal was positioned in the midline of the pre-pubic region. Females were positioned in right lateral recumbency to locate the left ovarian pedicle, and the uterine horn was held by a transcutaneous suture.The pedicle was cauterized and incised. The procedure was then performed on the contralateral ovary. The ovaries were exteriorized from the abdomen, along with the uterus, through the second access point. The uterine body was exposed, fixed and sectioned, and the abdominal incisions were sutured. RESULTS:Surgeries were performed in an average of 41.4±14.2 minutes. The main complications included hypotension (7.7%) and subcutaneous emphysema (7.7%), and 13.5% of the surgeries were converted to laparotomy. CONCLUSION:Ovariohysterectomy using a video-assisted technique and two access portals is safe, has minimal risks and is effective for the spaying of queens.

No abstract

Due to the high use of animals as experimental models for pharmaceutical trials, especially rats, it is necessary to standardize reference values for the evaluation of liver perfil by the analytes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and renal function, through the analytes urea and creatinine. For this purpose, samples were collected from 169 samples of Rattus norvegicus of Wistar strain, from the Vivarium of the University of Franca (SP), provided of semi-automated analytical methodology. For the calculation of the value, mean and standard deviation were performed for standardization and for the creation of reference values, therefore -2DP for minimum value determination and + 2DP for maximum value were used. The liver enzyme ALT presented a reference range from 21 to 96 U / L, while the AST from 44 to 186 U / L. In renal markers, urea was 20 to 73 mg / dL and creatinine 0.23 to 0.77 mg / dL. Given the applied methodology and the results obtained, it is admitted that it is very important to standardize the reference values of analytes in animal houses due to geographical changes, protocols, diets, among others; In addition, reducing the use of animals in experimental procedures in addition to guiding researchers on animal health and assisting analysis in various experimental research projects.

Diversas afecções orais podem acometer a espécie canina e felina, causando comprometimentos locais e sistêmicos. Assim, o objetivo do presente trabalho foi realizar estudo retrospectivo de 385 casos atendidos no setor cirúrgico de Odontologia de Pequenos Animais da Universidade de Franca, enfatizando os de fístulas infraorbitárias, fraturas dentárias e ósseas e neoplasias e, ademais, relacioná-los com a resenha dos pacientes (sexo, espécie, idade e raça) e tipo de alimentação. Os resultados foram expressos em percentuais. Das 385 fichas odontológicas, 30 pacientes foram diagnosticados com fístulas infraorbitárias (7,8%), 25 com fraturas dentárias (6,5%), 26 com fraturas ósseas (6,8%) e 28 com neoplasias (7,3%). Conforme descrito na literatura científica, não houve diferença significativa quanto ao gênero dos acometidos por todas as afecções orais. A espécie canina foi a mais afetada e a maioria dos pacientes era adultos a idosos. Os sem raça definida e os Poodles foram os mais acometidos. Quanto ao tipo de alimentação fornecida, a maioria era ração comercial; em contrapartida, grande parte oferecia ração comercial acrescida de comida caseira, o que pode predispor e agravar algumas enfermidades orais. Perante a metodologia preconizada e os dados obtidos, pode-se inferir que as fístulas infraorbitárias, fraturas dentárias, fraturas ósseas e neoplasias são comumente diagnosticadas em animais de companhia, principalmente em cães idosos e pequenos, podendo gerar danos locais e sistêmicos. A alimentação errônea pode predispor enfermidades orais; assim a divulgação aos tutores quanto aos cuidados odontológicos pode melhorar a qualidade de vida e sobrevida dos cães e gatos.

No abstract

Visceral leishmaniasis is an infectious disease of chronic, emerging and zoonotic nature that presents various degrees of severity. In Brazil, this illness is caused by Leishmania infantum (Leishmania chagasi), which is transmitted by the bite of the sand fly Lutzomyia longipalpis, and dogs are its main reservoir. Given the increasing spread of this disease across Brazil, the aim of this study was to report on six cases of canine visceral leishmaniasis, diagnosed in June 2013, in the city of Pedregulho, State of São Paulo, considered to be a non-endemic area and free of phlebotomine sand flies. The diagnosis was based on clinical signs of the patients and additional tests (serological and parasitological). It was concluded that the diagnosis of leishmaniasis is complex because the clinical signs are similar to other systemic diseases, thus justifying the importance of parasitological test of bone marrow, considered "gold standard", in the confirmation of the disease. In addition, the area was not, until now, considered risk place, despite notification.