Development and characterization of GR2E Golden rice introgression lines

Swamy, B. P. Mallikarjuna; Marundan, Severino; Samia, Mercy; Ordonio, Reynante Lacsamana; Rebong, Democrito B.; Miranda, Ronalyn T.; Alibuyog, Anielyn Y.; Rebong, Anna Theresa; Tabil, Ma. Angela; Suralta, Roel Rodriguez; Alfonso, Antonio A.; Biswas, P.; Kader, Md. Abdul; Reinke, Russell; Boncodin, Raul; MacKenzie, Donald J.

doi:10.1038/s41598-021-82001-0

Cited by 27 publications

(16 citation statements)

References 40 publications

(53 reference statements)

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“…Transgenic “Golden Rice” was bred by introducing Zmpsy1 and crtl into Kaybonnet, an US temperate japonica rice ( Ye et al., 2000 ; Paine et al., 2005 ). Thereafter, the “Golden Rice” (GR2E) characteristic was bred into Asian cultivars via marker-assisted backcross breeding ( Mallikarjuna Swamy et al., 2021 ). The milled rice from GR2E contributed up to 89%–113% and 57%–99% of the estimated average requirement for vitamin A for preschool children in Bangladesh and the Philippines, respectively, according to efficacy trials ( Mallikarjuna Swamy et al., 2019 ).…”

Section: Genetically Modified “Provitamin A” Biofortified Cropsmentioning

confidence: 99%

Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots

Dwivedi¹,

Garcia‐Oliveira

Govindaraj

et al. 2023

Front. Plant Sci.

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Malnutrition results in enormous socio-economic costs to the individual, their community, and the nation’s economy. The evidence suggests an overall negative impact of climate change on the agricultural productivity and nutritional quality of food crops. Producing more food with better nutritional quality, which is feasible, should be prioritized in crop improvement programs. Biofortification refers to developing micronutrient -dense cultivars through crossbreeding or genetic engineering. This review provides updates on nutrient acquisition, transport, and storage in plant organs; the cross-talk between macro- and micronutrients transport and signaling; nutrient profiling and spatial and temporal distribution; the putative and functionally characterized genes/single-nucleotide polymorphisms associated with Fe, Zn, and β-carotene; and global efforts to breed nutrient-dense crops and map adoption of such crops globally. This article also includes an overview on the bioavailability, bioaccessibility, and bioactivity of nutrients as well as the molecular basis of nutrient transport and absorption in human. Over 400 minerals (Fe, Zn) and provitamin A-rich cultivars have been released in the Global South. Approximately 4.6 million households currently cultivate Zn-rich rice and wheat, while ~3 million households in sub-Saharan Africa and Latin America benefit from Fe-rich beans, and 2.6 million people in sub-Saharan Africa and Brazil eat provitamin A-rich cassava. Furthermore, nutrient profiles can be improved through genetic engineering in an agronomically acceptable genetic background. The development of “Golden Rice” and provitamin A-rich dessert bananas and subsequent transfer of this trait into locally adapted cultivars are evident, with no significant change in nutritional profile, except for the trait incorporated. A greater understanding of nutrient transport and absorption may lead to the development of diet therapy for the betterment of human health.

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Section: Genetically Modified “Provitamin A” Biofortified Cropsmentioning

confidence: 99%

Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots

Dwivedi¹,

Garcia‐Oliveira

Govindaraj

et al. 2023

Front. Plant Sci.

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“…On the other hand, the presence of foreign genes in transgenic plants triggers biosafety regulations. However, a comparison of transgenic Golden rice GR2E and conventional rice showed no statistically significant differences in the concentrations of phytic acid or the levels of trypsin inhibitor and no differences in pest and disease reactions between them (Mallikarjuna Swamy et al, 2019;Mallikarjuna Swamy et al, 2021). Comparative assessment of transgenic wheat containing the sunflower gene, HaHB4 conferring drought tolerance and improved yield in driest environments, is nutritionally equivalent to non-transgenic wheat lines (González et al, 2019;Miranda et al, 2022).…”

Section: Transgenes and Crispr/cas9 Genome Editingmentioning

confidence: 99%

Sustaining yield and nutritional quality of peanuts in harsh environments: Physiological and molecular basis of drought and heat stress tolerance

et al. 2023

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Climate change is significantly impacting agricultural production worldwide. Peanuts provide food and nutritional security to millions of people across the globe because of its high nutritive values. Drought and heat stress alone or in combination cause substantial yield losses to peanut production. The stress, in addition, adversely impact nutritional quality. Peanuts exposed to drought stress at reproductive stage are prone to aflatoxin contamination, which imposes a restriction on use of peanuts as health food and also adversely impact peanut trade. A comprehensive understanding of the impact of drought and heat stress at physiological and molecular levels may accelerate the development of stress tolerant productive peanut cultivars adapted to a given production system. Significant progress has been achieved towards the characterization of germplasm for drought and heat stress tolerance, unlocking the physiological and molecular basis of stress tolerance, identifying significant marker-trait associations as well major QTLs and candidate genes associated with drought tolerance, which after validation may be deployed to initiate marker-assisted breeding for abiotic stress adaptation in peanut. The proof of concept about the use of transgenic technology to add value to peanuts has been demonstrated. Advances in phenomics and artificial intelligence to accelerate the timely and cost-effective collection of phenotyping data in large germplasm/breeding populations have also been discussed. Greater focus is needed to accelerate research on heat stress tolerance in peanut. A suits of technological innovations are now available in the breeders toolbox to enhance productivity and nutritional quality of peanuts in harsh environments. A holistic breeding approach that considers drought and heat-tolerant traits to simultaneously address both stresses could be a successful strategy to produce climate-resilient peanut genotypes with improved nutritional quality.

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“…There has been considerable progress in developing biofortified staple crops, predominantly via synthetic metabolic engineering [ 249 ]. The best-known example, and the first biofortified staple crop utilizing this method, is the β-carotene-enriched ‘Golden Rice’ [ 250 ]. The bioavailability of the β-carotene in rice, a precursor of provitamin A, could reduce vitamin A deficiency, which affects an estimated 190 million preschool-age children worldwide, of whom 91.5 million reside in Southeast Asia [ 251 ].…”

Section: Benefits Of Agricultural Biotechnologymentioning

confidence: 99%

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

Hamdan

Noor

Abd-Aziz

et al. 2022

Plants

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Technological applications in agriculture have evolved substantially to increase crop yields and quality to meet global food demand. Conventional techniques, such as seed saving, selective breeding, and mutation breeding (variation breeding), have dramatically increased crop production, especially during the ‘Green Revolution’ in the 1990s. However, newer issues, such as limited arable lands, climate change, and ever-increasing food demand, pose challenges to agricultural production and threaten food security. In the following ‘Gene Revolution’ era, rapid innovations in the biotechnology field provide alternative strategies to further improve crop yield, quality, and resilience towards biotic and abiotic stresses. These innovations include the introduction of DNA recombinant technology and applications of genome editing techniques, such as transcription activator-like effector (TALEN), zinc-finger nucleases (ZFN), and clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas) systems. However, the acceptance and future of these modern tools rely on the regulatory frameworks governing their development and production in various countries. Herein, we examine the evolution of technological applications in agriculture, focusing on the motivations for their introduction, technical challenges, possible benefits and concerns, and regulatory frameworks governing genetically engineered product development and production.

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Development and characterization of GR2E Golden rice introgression lines

Cited by 27 publications

References 40 publications

Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots

Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots

Sustaining yield and nutritional quality of peanuts in harsh environments: Physiological and molecular basis of drought and heat stress tolerance

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

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